Dynamic switching of transcriptional regulators between two distinct low-mobility chromatin states

Kaustubh Wagh, Diana A. Stavreva, Rikke A.M. Jensen, Ville Paakinaho, Gregory Fettweis, R. Louis Schiltz, Daniel Wüstner, Susanne Mandrup, Diego M. Presman, Arpita Upadhyaya, Gordon L. Hager

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

27 Downloads (Pure)

Abstract

How chromatin dynamics relate to transcriptional activity remains poorly understood. Using single-molecule tracking, coupled with machine learning, we show that histone H2B and multiple chromatin-bound transcriptional regulators display two distinct low-mobility states. Ligand activation results in a marked increase in the propensity of steroid receptors to bind in the lowest-mobility state. Mutational analysis revealed that interactions with chromatin in the lowest-mobility state require an intact DNA binding domain and oligomerization domains. These states are not spatially separated as previously believed, but individual H2B and bound-TF molecules can dynamically switch between them on time scales of seconds. Single bound-TF molecules with different mobilities exhibit different dwell time distributions, suggesting that the mobility of TFs is intimately coupled with their binding dynamics. Together, our results identify two unique and distinct low-mobility states that appear to represent common pathways for transcription activation in mammalian cells.

OriginalsprogEngelsk
Artikelnummereade1122
TidsskriftScience Advances
Vol/bind9
Udgave nummer24
Antal sider19
ISSN2375-2548
DOI
StatusUdgivet - 14. jun. 2023

Fingeraftryk

Dyk ned i forskningsemnerne om 'Dynamic switching of transcriptional regulators between two distinct low-mobility chromatin states'. Sammen danner de et unikt fingeraftryk.

Citationsformater