TY - JOUR
T1 - Dynamic Rewiring of Promoter-Anchored Chromatin Loops during Adipocyte Differentiation
AU - Siersbæk, Rasmus
AU - Madsen, Jesper Grud Skat
AU - Javierre, Biola Maria
AU - Nielsen, Ronni
AU - Bagge, Emilie Kristine
AU - Cairns, Jonathan
AU - Wingett, Steven William
AU - Traynor, Sofie
AU - Spivakov, Mikhail
AU - Fraser, Peter
AU - Mandrup, Susanne
N1 - Copyright © 2017 Elsevier Inc. All rights reserved.
PY - 2017
Y1 - 2017
N2 - Interactions between transcriptional promoters and their distal regulatory elements play an important role in transcriptional regulation; however, the extent to which these interactions are subject to rapid modulations in response to signals is unknown. Here, we use promoter capture Hi-C to demonstrate a rapid reorganization of promoter-anchored chromatin loops within 4 hr after inducing differentiation of 3T3-L1 preadipocytes. The establishment of new promoter-enhancer loops is tightly coupled to activation of poised (histone H3 lysine 4 mono- and dimethylated) enhancers, as evidenced by the acquisition of histone H3 lysine 27 acetylation and the binding of MED1, SMC1, and P300 proteins to these regions, as well as to activation of target genes. Intriguingly, formation of loops connecting activated enhancers and promoters is also associated with extensive recruitment of corepressors such as NCoR and HDACs, indicating that this class of coregulators may play a previously unrecognized role during enhancer activation.
AB - Interactions between transcriptional promoters and their distal regulatory elements play an important role in transcriptional regulation; however, the extent to which these interactions are subject to rapid modulations in response to signals is unknown. Here, we use promoter capture Hi-C to demonstrate a rapid reorganization of promoter-anchored chromatin loops within 4 hr after inducing differentiation of 3T3-L1 preadipocytes. The establishment of new promoter-enhancer loops is tightly coupled to activation of poised (histone H3 lysine 4 mono- and dimethylated) enhancers, as evidenced by the acquisition of histone H3 lysine 27 acetylation and the binding of MED1, SMC1, and P300 proteins to these regions, as well as to activation of target genes. Intriguingly, formation of loops connecting activated enhancers and promoters is also associated with extensive recruitment of corepressors such as NCoR and HDACs, indicating that this class of coregulators may play a previously unrecognized role during enhancer activation.
KW - 3T3-L1 Cells
KW - Adipocytes
KW - Adipogenesis
KW - Animals
KW - Cell Cycle Proteins
KW - Chromatin
KW - Chromatin Assembly and Disassembly
KW - Chromatin Immunoprecipitation
KW - Chromosomal Proteins, Non-Histone
KW - E1A-Associated p300 Protein
KW - Enhancer Elements, Genetic
KW - Mediator Complex Subunit 1
KW - Mice
KW - Nucleic Acid Conformation
KW - Promoter Regions, Genetic
KW - RNA, Messenger
KW - Sequence Analysis, RNA
KW - Time Factors
KW - Transcription, Genetic
KW - Transcriptional Activation
KW - Journal Article
U2 - 10.1016/j.molcel.2017.04.010
DO - 10.1016/j.molcel.2017.04.010
M3 - Journal article
C2 - 28475875
SN - 1097-2765
VL - 66
SP - 420-435.e5
JO - Molecular Cell
JF - Molecular Cell
IS - 3
ER -