Dominance of a clonal green sulfur bacterial population in a stratified lake

Lea H. Gregersen, Kirsten Silvia Habicht, Sandro Peduzzi, Mauro Tonolla, Donald Eugene Canfield, Mette Miller, Raymond Pickett Cox, Niels-Ulrik Frigaard

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

For many years, the chemocline of the meromictic Lake Cadagno, Switzerland, was dominated by purple sulfur bacteria. However, following a major community shift in recent years, green sulfur bacteria (GSB) have come to dominate. We investigated this community by performing microbial diversity surveys using FISH cell counting and population multilocus sequence typing [clone library sequence analysis of the small subunit (SSU) rRNA locus and two loci involved in photosynthesis in GSB: fmoA and csmCA]. All bacterial populations clearly stratified according to water column chemistry. The GSB population peaked in the chemocline (c. 8 × 106 GSB cells mL−1) and constituted about 50% of all cells in the anoxic zones of the water column. At least 99.5% of these GSB cells had SSU rRNA, fmoA, and csmCA sequences essentially identical to that of the previously isolated and genome-sequenced GSB Chlorobium clathratiforme strain BU-1 (DSM 5477). This ribotype was not detected in Lake Cadagno before the bloom of GSB. These observations suggest that the C. clathratiforme population that has stabilized in Lake Cadagno is clonal. We speculate that such a clonal bloom could be caused by environmental disturbance, mutational adaptation, or invasion.
OriginalsprogEngelsk
TidsskriftFEMS Microbiology Ecology
Vol/bind70
Udgave nummer1
Sider (fra-til)30-41
ISSN0168-6496
DOI
StatusUdgivet - 2009

Fingeraftryk

Lakes
sulfur
bacterium
lake
Population
Chlorobium
algal bloom
water column
Ribotyping
meromictic lake
environmental disturbance
Sequence Analysis
Clone Cells
clone
photosynthesis
genome

Citer dette

Gregersen, L. H., Habicht, K. S., Peduzzi, S., Tonolla, M., Canfield, D. E., Miller, M., ... Frigaard, N-U. (2009). Dominance of a clonal green sulfur bacterial population in a stratified lake. FEMS Microbiology Ecology, 70(1), 30-41. https://doi.org/10.1111/j.1574-6941.2009.00737.x
Gregersen, Lea H. ; Habicht, Kirsten Silvia ; Peduzzi, Sandro ; Tonolla, Mauro ; Canfield, Donald Eugene ; Miller, Mette ; Cox, Raymond Pickett ; Frigaard, Niels-Ulrik. / Dominance of a clonal green sulfur bacterial population in a stratified lake. I: FEMS Microbiology Ecology. 2009 ; Bind 70, Nr. 1. s. 30-41.
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title = "Dominance of a clonal green sulfur bacterial population in a stratified lake",
abstract = "For many years, the chemocline of the meromictic Lake Cadagno, Switzerland, was dominated by purple sulfur bacteria. However, following a major community shift in recent years, green sulfur bacteria (GSB) have come to dominate. We investigated this community by performing microbial diversity surveys using FISH cell counting and population multilocus sequence typing [clone library sequence analysis of the small subunit (SSU) rRNA locus and two loci involved in photosynthesis in GSB: fmoA and csmCA]. All bacterial populations clearly stratified according to water column chemistry. The GSB population peaked in the chemocline (c. 8 × 106 GSB cells mL−1) and constituted about 50{\%} of all cells in the anoxic zones of the water column. At least 99.5{\%} of these GSB cells had SSU rRNA, fmoA, and csmCA sequences essentially identical to that of the previously isolated and genome-sequenced GSB Chlorobium clathratiforme strain BU-1 (DSM 5477). This ribotype was not detected in Lake Cadagno before the bloom of GSB. These observations suggest that the C. clathratiforme population that has stabilized in Lake Cadagno is clonal. We speculate that such a clonal bloom could be caused by environmental disturbance, mutational adaptation, or invasion.",
author = "Gregersen, {Lea H.} and Habicht, {Kirsten Silvia} and Sandro Peduzzi and Mauro Tonolla and Canfield, {Donald Eugene} and Mette Miller and Cox, {Raymond Pickett} and Niels-Ulrik Frigaard",
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Gregersen, LH, Habicht, KS, Peduzzi, S, Tonolla, M, Canfield, DE, Miller, M, Cox, RP & Frigaard, N-U 2009, 'Dominance of a clonal green sulfur bacterial population in a stratified lake', FEMS Microbiology Ecology, bind 70, nr. 1, s. 30-41. https://doi.org/10.1111/j.1574-6941.2009.00737.x

Dominance of a clonal green sulfur bacterial population in a stratified lake. / Gregersen, Lea H.; Habicht, Kirsten Silvia; Peduzzi, Sandro; Tonolla, Mauro; Canfield, Donald Eugene; Miller, Mette; Cox, Raymond Pickett; Frigaard, Niels-Ulrik.

I: FEMS Microbiology Ecology, Bind 70, Nr. 1, 2009, s. 30-41.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Dominance of a clonal green sulfur bacterial population in a stratified lake

AU - Gregersen, Lea H.

AU - Habicht, Kirsten Silvia

AU - Peduzzi, Sandro

AU - Tonolla, Mauro

AU - Canfield, Donald Eugene

AU - Miller, Mette

AU - Cox, Raymond Pickett

AU - Frigaard, Niels-Ulrik

N1 - Paper id:: DOI: 10.1111/j.1574-6941.2009.00737.x

PY - 2009

Y1 - 2009

N2 - For many years, the chemocline of the meromictic Lake Cadagno, Switzerland, was dominated by purple sulfur bacteria. However, following a major community shift in recent years, green sulfur bacteria (GSB) have come to dominate. We investigated this community by performing microbial diversity surveys using FISH cell counting and population multilocus sequence typing [clone library sequence analysis of the small subunit (SSU) rRNA locus and two loci involved in photosynthesis in GSB: fmoA and csmCA]. All bacterial populations clearly stratified according to water column chemistry. The GSB population peaked in the chemocline (c. 8 × 106 GSB cells mL−1) and constituted about 50% of all cells in the anoxic zones of the water column. At least 99.5% of these GSB cells had SSU rRNA, fmoA, and csmCA sequences essentially identical to that of the previously isolated and genome-sequenced GSB Chlorobium clathratiforme strain BU-1 (DSM 5477). This ribotype was not detected in Lake Cadagno before the bloom of GSB. These observations suggest that the C. clathratiforme population that has stabilized in Lake Cadagno is clonal. We speculate that such a clonal bloom could be caused by environmental disturbance, mutational adaptation, or invasion.

AB - For many years, the chemocline of the meromictic Lake Cadagno, Switzerland, was dominated by purple sulfur bacteria. However, following a major community shift in recent years, green sulfur bacteria (GSB) have come to dominate. We investigated this community by performing microbial diversity surveys using FISH cell counting and population multilocus sequence typing [clone library sequence analysis of the small subunit (SSU) rRNA locus and two loci involved in photosynthesis in GSB: fmoA and csmCA]. All bacterial populations clearly stratified according to water column chemistry. The GSB population peaked in the chemocline (c. 8 × 106 GSB cells mL−1) and constituted about 50% of all cells in the anoxic zones of the water column. At least 99.5% of these GSB cells had SSU rRNA, fmoA, and csmCA sequences essentially identical to that of the previously isolated and genome-sequenced GSB Chlorobium clathratiforme strain BU-1 (DSM 5477). This ribotype was not detected in Lake Cadagno before the bloom of GSB. These observations suggest that the C. clathratiforme population that has stabilized in Lake Cadagno is clonal. We speculate that such a clonal bloom could be caused by environmental disturbance, mutational adaptation, or invasion.

U2 - 10.1111/j.1574-6941.2009.00737.x

DO - 10.1111/j.1574-6941.2009.00737.x

M3 - Journal article

VL - 70

SP - 30

EP - 41

JO - F E M S Microbiology Ecology

JF - F E M S Microbiology Ecology

SN - 0168-6496

IS - 1

ER -