Abstract
Background
The forkhead box P3 gene (FOXP3) is the master gene regulatory of T regulatory cells. The two most abundantly expressed isoforms (FOXP3fl and FOXP3d2) are essential for T regulatory cell function. Studies have shown that kidney transplant recipients with higher levels of FOXP3 show stable allograft function despite negligible immunosuppressive therapy, but the association between pre-transplant FOXP3 splice variants and baseline allograft function remains unclear.
Methods
We collected blood samples from kidney transplant candidates who were subsequently transplanted at Odense University Hospital. Complementary DNA was synthesized from peripheral blood mononuclear cell ribonucleic acid. Using quantitative polymerase chain reaction, we measured pre-mRNA-FOXP3, FOXP3fl, FOXP3d2 and total FOXP3 (FOXP3fl and FOXP3d2). Baseline estimated glomerular filtration rate (eGFR) was determined as the mean of measurements performed within 62 to 76 days post-transplant, and 104 to 114 days post-transplant. We assessed the association between FOXP3 levels and mean baseline eGFR with linear regression adjusted for recipient age, sex, prior kidney transplantation and underlying cause of kidney disease.
Results
In 230 kidney transplant recipients, the median eGFR was 51 [IQR 39, 61]. Median logarithmic values of FOXP3 variants were: Total FOXP3 -3.31 [IQR -3.52, -3.10], pre-mRNA-FOXP3 -4.28 [4.48, -4.09], FOXP3fl -3.68 [-3.93, -3.42], FOXP3d2 -3.70 [-4.03, -3.44]. We observed a significant association between lower baseline eGFR and increasing recipient age (estimate -0.43±0.07 standard error, p
Conclusion
Pre-transplant FOXP3 splice variants are not associated with baseline eGFR.
The forkhead box P3 gene (FOXP3) is the master gene regulatory of T regulatory cells. The two most abundantly expressed isoforms (FOXP3fl and FOXP3d2) are essential for T regulatory cell function. Studies have shown that kidney transplant recipients with higher levels of FOXP3 show stable allograft function despite negligible immunosuppressive therapy, but the association between pre-transplant FOXP3 splice variants and baseline allograft function remains unclear.
Methods
We collected blood samples from kidney transplant candidates who were subsequently transplanted at Odense University Hospital. Complementary DNA was synthesized from peripheral blood mononuclear cell ribonucleic acid. Using quantitative polymerase chain reaction, we measured pre-mRNA-FOXP3, FOXP3fl, FOXP3d2 and total FOXP3 (FOXP3fl and FOXP3d2). Baseline estimated glomerular filtration rate (eGFR) was determined as the mean of measurements performed within 62 to 76 days post-transplant, and 104 to 114 days post-transplant. We assessed the association between FOXP3 levels and mean baseline eGFR with linear regression adjusted for recipient age, sex, prior kidney transplantation and underlying cause of kidney disease.
Results
In 230 kidney transplant recipients, the median eGFR was 51 [IQR 39, 61]. Median logarithmic values of FOXP3 variants were: Total FOXP3 -3.31 [IQR -3.52, -3.10], pre-mRNA-FOXP3 -4.28 [4.48, -4.09], FOXP3fl -3.68 [-3.93, -3.42], FOXP3d2 -3.70 [-4.03, -3.44]. We observed a significant association between lower baseline eGFR and increasing recipient age (estimate -0.43±0.07 standard error, p
Conclusion
Pre-transplant FOXP3 splice variants are not associated with baseline eGFR.
| Originalsprog | Engelsk |
|---|---|
| Publikationsdato | 15. sep. 2023 |
| Status | Udgivet - 15. sep. 2023 |
| Begivenhed | 37th Biennial Congress of the Nordic Society of Nephrology - Iceland, Reykjavik, Island Varighed: 13. sep. 2023 → 16. sep. 2023 https://nsn2023.is/ |
Konference
| Konference | 37th Biennial Congress of the Nordic Society of Nephrology |
|---|---|
| Lokation | Iceland |
| Land/Område | Island |
| By | Reykjavik |
| Periode | 13/09/2023 → 16/09/2023 |
| Internetadresse |