DNA repair in plant mitochondria - A complete base excision repair pathway in potato tuber mitochondria

Beatriz Ferrando, Ana Luiza Dorigan de Matos Furlanetto, Ricardo Gredilla, Jesper F Havelund, Kim H Hebelstrup, Ian Max Møller*, Tinna Stevnsner

*Kontaktforfatter for dette arbejde

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Resumé

Mitochondria are one of the major sites of reactive oxygen species (ROS) production in the plant cell. ROS can damage DNA, and this damage is in many organisms mainly repaired by the base excision repair (BER) pathway. We know very little about DNA repair in plants especially in the mitochondria. Combining proteomics, bioinformatics, Western blot and enzyme assays we here demonstrate that the complete BER pathway is found in mitochondria isolated from potato (Solanum tubersosum) tubers. The enzyme activities of three DNA glycosylases and an apurinic/apyrimidinic (AP) endonuclease were characterized with respect to Mg2+ dependence and, in the case of the AP endonuclease, temperature sensitivity. Evidence for the presence of the DNA polymerase and the DNA ligase, which complete the repair pathway by replacing the excised base and closing the gap, was also obtained. We tested the effect of oxidative stress on the mitochondrial BER pathway by incubating potato tubers under hypoxia. Protein carbonylation increased significantly in hypoxic tuber mitochondria indicative of increased oxidative stress. The activity of two BER enzymes increased significantly in response to this oxidative stress consistent with the role of the BER pathway in the repair of oxidative damage to mtDNA. This article is protected by copyright. All rights reserved.

OriginalsprogEngelsk
TidsskriftPhysiologia Plantarum
Vol/bind166
Udgave nummer2
Sider (fra-til)494-512
ISSN0031-9317
DOI
StatusUdgivet - jun. 2019

Fingeraftryk

DNA repair
tubers
mitochondria
potatoes
oxidative stress
reactive oxygen species
Reactive Oxygen Species
Protein Carbonylation
glycosylases
DNA Ligases
Endonucleases
DNA-directed DNA polymerase
Plant Cells
DNA
Solanum
Enzymes
enzymes
Computational Biology
ligases
bioinformatics

Citer dette

Ferrando, B., de Matos Furlanetto, A. L. D., Gredilla, R., Havelund, J. F., Hebelstrup, K. H., Møller, I. M., & Stevnsner, T. (2019). DNA repair in plant mitochondria - A complete base excision repair pathway in potato tuber mitochondria. Physiologia Plantarum, 166(2), 494-512. https://doi.org/10.1111/ppl.12801
Ferrando, Beatriz ; de Matos Furlanetto, Ana Luiza Dorigan ; Gredilla, Ricardo ; Havelund, Jesper F ; Hebelstrup, Kim H ; Møller, Ian Max ; Stevnsner, Tinna. / DNA repair in plant mitochondria - A complete base excision repair pathway in potato tuber mitochondria. I: Physiologia Plantarum. 2019 ; Bind 166, Nr. 2. s. 494-512.
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title = "DNA repair in plant mitochondria - A complete base excision repair pathway in potato tuber mitochondria",
abstract = "Mitochondria are one of the major sites of reactive oxygen species (ROS) production in the plant cell. ROS can damage DNA, and this damage is in many organisms mainly repaired by the base excision repair (BER) pathway. We know very little about DNA repair in plants especially in the mitochondria. Combining proteomics, bioinformatics, Western blot and enzyme assays we here demonstrate that the complete BER pathway is found in mitochondria isolated from potato (Solanum tubersosum) tubers. The enzyme activities of three DNA glycosylases and an apurinic/apyrimidinic (AP) endonuclease were characterized with respect to Mg2+ dependence and, in the case of the AP endonuclease, temperature sensitivity. Evidence for the presence of the DNA polymerase and the DNA ligase, which complete the repair pathway by replacing the excised base and closing the gap, was also obtained. We tested the effect of oxidative stress on the mitochondrial BER pathway by incubating potato tubers under hypoxia. Protein carbonylation increased significantly in hypoxic tuber mitochondria indicative of increased oxidative stress. The activity of two BER enzymes increased significantly in response to this oxidative stress consistent with the role of the BER pathway in the repair of oxidative damage to mtDNA. This article is protected by copyright. All rights reserved.",
keywords = "DNA Glycosylases/genetics, DNA Repair/genetics, DNA, Mitochondrial/genetics, DNA, Plant/genetics, DNA-(Apurinic or Apyrimidinic Site) Lyase/genetics, DNA-Directed DNA Polymerase/genetics, Mitochondria/genetics, Reactive Oxygen Species/metabolism, Solanum tuberosum/genetics",
author = "Beatriz Ferrando and {de Matos Furlanetto}, {Ana Luiza Dorigan} and Ricardo Gredilla and Havelund, {Jesper F} and Hebelstrup, {Kim H} and M{\o}ller, {Ian Max} and Tinna Stevnsner",
note = "This article is protected by copyright. All rights reserved.",
year = "2019",
month = "6",
doi = "10.1111/ppl.12801",
language = "English",
volume = "166",
pages = "494--512",
journal = "Physiologia Plantarum",
issn = "0031-9317",
publisher = "Wiley-Blackwell",
number = "2",

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Ferrando, B, de Matos Furlanetto, ALD, Gredilla, R, Havelund, JF, Hebelstrup, KH, Møller, IM & Stevnsner, T 2019, 'DNA repair in plant mitochondria - A complete base excision repair pathway in potato tuber mitochondria', Physiologia Plantarum, bind 166, nr. 2, s. 494-512. https://doi.org/10.1111/ppl.12801

DNA repair in plant mitochondria - A complete base excision repair pathway in potato tuber mitochondria. / Ferrando, Beatriz; de Matos Furlanetto, Ana Luiza Dorigan; Gredilla, Ricardo; Havelund, Jesper F; Hebelstrup, Kim H; Møller, Ian Max; Stevnsner, Tinna.

I: Physiologia Plantarum, Bind 166, Nr. 2, 06.2019, s. 494-512.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - DNA repair in plant mitochondria - A complete base excision repair pathway in potato tuber mitochondria

AU - Ferrando, Beatriz

AU - de Matos Furlanetto, Ana Luiza Dorigan

AU - Gredilla, Ricardo

AU - Havelund, Jesper F

AU - Hebelstrup, Kim H

AU - Møller, Ian Max

AU - Stevnsner, Tinna

N1 - This article is protected by copyright. All rights reserved.

PY - 2019/6

Y1 - 2019/6

N2 - Mitochondria are one of the major sites of reactive oxygen species (ROS) production in the plant cell. ROS can damage DNA, and this damage is in many organisms mainly repaired by the base excision repair (BER) pathway. We know very little about DNA repair in plants especially in the mitochondria. Combining proteomics, bioinformatics, Western blot and enzyme assays we here demonstrate that the complete BER pathway is found in mitochondria isolated from potato (Solanum tubersosum) tubers. The enzyme activities of three DNA glycosylases and an apurinic/apyrimidinic (AP) endonuclease were characterized with respect to Mg2+ dependence and, in the case of the AP endonuclease, temperature sensitivity. Evidence for the presence of the DNA polymerase and the DNA ligase, which complete the repair pathway by replacing the excised base and closing the gap, was also obtained. We tested the effect of oxidative stress on the mitochondrial BER pathway by incubating potato tubers under hypoxia. Protein carbonylation increased significantly in hypoxic tuber mitochondria indicative of increased oxidative stress. The activity of two BER enzymes increased significantly in response to this oxidative stress consistent with the role of the BER pathway in the repair of oxidative damage to mtDNA. This article is protected by copyright. All rights reserved.

AB - Mitochondria are one of the major sites of reactive oxygen species (ROS) production in the plant cell. ROS can damage DNA, and this damage is in many organisms mainly repaired by the base excision repair (BER) pathway. We know very little about DNA repair in plants especially in the mitochondria. Combining proteomics, bioinformatics, Western blot and enzyme assays we here demonstrate that the complete BER pathway is found in mitochondria isolated from potato (Solanum tubersosum) tubers. The enzyme activities of three DNA glycosylases and an apurinic/apyrimidinic (AP) endonuclease were characterized with respect to Mg2+ dependence and, in the case of the AP endonuclease, temperature sensitivity. Evidence for the presence of the DNA polymerase and the DNA ligase, which complete the repair pathway by replacing the excised base and closing the gap, was also obtained. We tested the effect of oxidative stress on the mitochondrial BER pathway by incubating potato tubers under hypoxia. Protein carbonylation increased significantly in hypoxic tuber mitochondria indicative of increased oxidative stress. The activity of two BER enzymes increased significantly in response to this oxidative stress consistent with the role of the BER pathway in the repair of oxidative damage to mtDNA. This article is protected by copyright. All rights reserved.

KW - DNA Glycosylases/genetics

KW - DNA Repair/genetics

KW - DNA, Mitochondrial/genetics

KW - DNA, Plant/genetics

KW - DNA-(Apurinic or Apyrimidinic Site) Lyase/genetics

KW - DNA-Directed DNA Polymerase/genetics

KW - Mitochondria/genetics

KW - Reactive Oxygen Species/metabolism

KW - Solanum tuberosum/genetics

U2 - 10.1111/ppl.12801

DO - 10.1111/ppl.12801

M3 - Journal article

C2 - 30035320

VL - 166

SP - 494

EP - 512

JO - Physiologia Plantarum

JF - Physiologia Plantarum

SN - 0031-9317

IS - 2

ER -