DNA-Mediated Liposome Fusion Observed by Fluorescence Spectrometry

Philipp M.G. Löffler, Oliver Ries, Stefan Vogel*

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Publikation: Kapitel i bog/rapport/konference-proceedingKapitel i bogForskningpeer review

Abstrakt

DNA-programmed and controlled fusion of lipid membranes have recently been optimized to reliably mix the contents between two populations of liposomes, each functionalized with complementary lipidated DNA (LiNA) oligomer. In this chapter we describe a procedure for DNA-controlled fusion of liposomes mediated by LiNAs that are designed to force bilayers into close proximity. Using a self-quenching fluorescent dye (Sulforhodamine B) to monitor both the mixing of the internal volumes and leakage of the dye into the outer volume we measure the efficiency of content mixing in the bulk population, allowing for direct comparison between different LiNA designs. By generating samples for calibration corresponding to different amounts of content mixing, the average number of fusion events per labeled liposome can be estimated.

OriginalsprogEngelsk
TitelNucleic Acid Detection and Structural Investigations : Methods and Protocols
RedaktørerKira Astakhova, Syeda Atia Bukhari
Antal sider18
Vol/bind2063
ForlagHumana Press
Publikationsdato2020
Sider101-118
Kapitel9
ISBN (Trykt)978-1-0716-0137-2
ISBN (Elektronisk)978-1-0716-0138-9
DOI
StatusUdgivet - 2020
NavnMethods in Molecular Biology
Vol/bind2063
ISSN1064-3745

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