DMBT1 expression is down-regulated in breast cancer.

P Braidotti, P G Nuciforo, J Mollenhauer, A Poustka, C Pellegrini, A Moro, G Bulfamante, G Coggi, S Bosari, G G Pietra

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2004-Aug-9
OriginalsprogEngelsk
TidsskriftBMC Cancer
Vol/bind4
Sider (fra-til)46
ISSN1471-2407
DOI
StatusUdgivet - 9. aug. 2004

Fingeraftryk

Epithelium
Cell Cycle
Polymerase Chain Reaction
Licensure
Proteins
Up-Regulation
Down-Regulation
Cell Proliferation
Messenger RNA
Neoplasms

Citer dette

Braidotti, P., Nuciforo, P. G., Mollenhauer, J., Poustka, A., Pellegrini, C., Moro, A., ... Pietra, G. G. (2004). DMBT1 expression is down-regulated in breast cancer. BMC Cancer, 4, 46. https://doi.org/10.1186/1471-2407-4-46
Braidotti, P ; Nuciforo, P G ; Mollenhauer, J ; Poustka, A ; Pellegrini, C ; Moro, A ; Bulfamante, G ; Coggi, G ; Bosari, S ; Pietra, G G. / DMBT1 expression is down-regulated in breast cancer. I: BMC Cancer. 2004 ; Bind 4. s. 46.
@article{ac9c11a057fb11ddb1a1000ea68e967b,
title = "DMBT1 expression is down-regulated in breast cancer.",
abstract = "BACKGROUND: We studied the expression of DMBT1 (deleted in malignant brain tumor 1), a putative tumor suppressor gene, in normal, proliferative, and malignant breast epithelium and its possible relation to cell cycle. METHODS: Sections from 17 benign lesions and 55 carcinomas were immunostained with anti DMBT1 antibody (DMBTh12) and sections from 36 samples, were double-stained also with anti MCM5, one of the 6 pre-replicative complex proteins with cell proliferation-licensing functions. DMBT1 gene expression at mRNA level was assessed by RT-PCR in frozen tissues samples from 39 patients. RESULTS: Normal glands and hyperplastic epithelium in benign lesions displayed a luminal polarized DMBTh12 immunoreactivity. Normal and hyperplastic epithelium adjacent to carcinomas showed a loss of polarization, with immunostaining present in basal and perinuclear cytoplasmic compartments. DMBT1 protein expression was down-regulated in the cancerous lesions compared to the normal and/or hyperplastic epithelium adjacent to carcinomas (3/55 positive carcinomas versus 33/42 positive normal/hyperplastic epithelia; p = 0.0001). In 72{\%} of cases RT-PCR confirmed immunohistochemical results. Most of normal and hyperplastic mammary cells positive with DMBTh12 were also MCM5-positive. CONCLUSIONS: The redistribution and up-regulation of DMBT1 in normal and hyperplastic tissues flanking malignant tumours and its down-regulation in carcinomas suggests a potential role in breast cancer. Moreover, the concomitant expression of DMTB1 and MCM5 suggests its possible association with the cell-cycle regulation.",
keywords = "Agglutinins, Breast, Breast Neoplasms, Carcinoma, Cell Cycle, Cell Cycle Proteins, Cell Line, Tumor, Cytoplasm, Down-Regulation, Epithelium, Humans, Hyperplasia, Immunohistochemistry, RNA, Messenger, Receptors, Cell Surface, Reverse Transcriptase Polymerase Chain Reaction",
author = "P Braidotti and Nuciforo, {P G} and J Mollenhauer and A Poustka and C Pellegrini and A Moro and G Bulfamante and G Coggi and S Bosari and Pietra, {G G}",
year = "2004",
month = "8",
day = "9",
doi = "10.1186/1471-2407-4-46",
language = "English",
volume = "4",
pages = "46",
journal = "B M C Cancer",
issn = "1471-2407",
publisher = "BioMed Central",

}

Braidotti, P, Nuciforo, PG, Mollenhauer, J, Poustka, A, Pellegrini, C, Moro, A, Bulfamante, G, Coggi, G, Bosari, S & Pietra, GG 2004, 'DMBT1 expression is down-regulated in breast cancer.', BMC Cancer, bind 4, s. 46. https://doi.org/10.1186/1471-2407-4-46

DMBT1 expression is down-regulated in breast cancer. / Braidotti, P; Nuciforo, P G; Mollenhauer, J; Poustka, A; Pellegrini, C; Moro, A; Bulfamante, G; Coggi, G; Bosari, S; Pietra, G G.

I: BMC Cancer, Bind 4, 09.08.2004, s. 46.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - DMBT1 expression is down-regulated in breast cancer.

AU - Braidotti, P

AU - Nuciforo, P G

AU - Mollenhauer, J

AU - Poustka, A

AU - Pellegrini, C

AU - Moro, A

AU - Bulfamante, G

AU - Coggi, G

AU - Bosari, S

AU - Pietra, G G

PY - 2004/8/9

Y1 - 2004/8/9

N2 - BACKGROUND: We studied the expression of DMBT1 (deleted in malignant brain tumor 1), a putative tumor suppressor gene, in normal, proliferative, and malignant breast epithelium and its possible relation to cell cycle. METHODS: Sections from 17 benign lesions and 55 carcinomas were immunostained with anti DMBT1 antibody (DMBTh12) and sections from 36 samples, were double-stained also with anti MCM5, one of the 6 pre-replicative complex proteins with cell proliferation-licensing functions. DMBT1 gene expression at mRNA level was assessed by RT-PCR in frozen tissues samples from 39 patients. RESULTS: Normal glands and hyperplastic epithelium in benign lesions displayed a luminal polarized DMBTh12 immunoreactivity. Normal and hyperplastic epithelium adjacent to carcinomas showed a loss of polarization, with immunostaining present in basal and perinuclear cytoplasmic compartments. DMBT1 protein expression was down-regulated in the cancerous lesions compared to the normal and/or hyperplastic epithelium adjacent to carcinomas (3/55 positive carcinomas versus 33/42 positive normal/hyperplastic epithelia; p = 0.0001). In 72% of cases RT-PCR confirmed immunohistochemical results. Most of normal and hyperplastic mammary cells positive with DMBTh12 were also MCM5-positive. CONCLUSIONS: The redistribution and up-regulation of DMBT1 in normal and hyperplastic tissues flanking malignant tumours and its down-regulation in carcinomas suggests a potential role in breast cancer. Moreover, the concomitant expression of DMTB1 and MCM5 suggests its possible association with the cell-cycle regulation.

AB - BACKGROUND: We studied the expression of DMBT1 (deleted in malignant brain tumor 1), a putative tumor suppressor gene, in normal, proliferative, and malignant breast epithelium and its possible relation to cell cycle. METHODS: Sections from 17 benign lesions and 55 carcinomas were immunostained with anti DMBT1 antibody (DMBTh12) and sections from 36 samples, were double-stained also with anti MCM5, one of the 6 pre-replicative complex proteins with cell proliferation-licensing functions. DMBT1 gene expression at mRNA level was assessed by RT-PCR in frozen tissues samples from 39 patients. RESULTS: Normal glands and hyperplastic epithelium in benign lesions displayed a luminal polarized DMBTh12 immunoreactivity. Normal and hyperplastic epithelium adjacent to carcinomas showed a loss of polarization, with immunostaining present in basal and perinuclear cytoplasmic compartments. DMBT1 protein expression was down-regulated in the cancerous lesions compared to the normal and/or hyperplastic epithelium adjacent to carcinomas (3/55 positive carcinomas versus 33/42 positive normal/hyperplastic epithelia; p = 0.0001). In 72% of cases RT-PCR confirmed immunohistochemical results. Most of normal and hyperplastic mammary cells positive with DMBTh12 were also MCM5-positive. CONCLUSIONS: The redistribution and up-regulation of DMBT1 in normal and hyperplastic tissues flanking malignant tumours and its down-regulation in carcinomas suggests a potential role in breast cancer. Moreover, the concomitant expression of DMTB1 and MCM5 suggests its possible association with the cell-cycle regulation.

KW - Agglutinins

KW - Breast

KW - Breast Neoplasms

KW - Carcinoma

KW - Cell Cycle

KW - Cell Cycle Proteins

KW - Cell Line, Tumor

KW - Cytoplasm

KW - Down-Regulation

KW - Epithelium

KW - Humans

KW - Hyperplasia

KW - Immunohistochemistry

KW - RNA, Messenger

KW - Receptors, Cell Surface

KW - Reverse Transcriptase Polymerase Chain Reaction

U2 - 10.1186/1471-2407-4-46

DO - 10.1186/1471-2407-4-46

M3 - Journal article

C2 - 15301691

VL - 4

SP - 46

JO - B M C Cancer

JF - B M C Cancer

SN - 1471-2407

ER -

Braidotti P, Nuciforo PG, Mollenhauer J, Poustka A, Pellegrini C, Moro A et al. DMBT1 expression is down-regulated in breast cancer. BMC Cancer. 2004 aug 9;4:46. https://doi.org/10.1186/1471-2407-4-46