DMBT1 expression distinguishes anorectal from cutaneous melanoma

Burkhard Maria Helmke, Marcus Renner, Annemarie Poustka, Peter Schirmacher, Jan Mollenhauer, Michael André Kern

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    AIMS: Anorectal melanoma (AM) forms a rare but highly malignant subset of mucosal melanoma with an extremely poor prognosis. Although AMs display histological and immunohistochemical features very similar to cutaneous melanoma (CM), no association exists either with exposure to ultraviolet light or with melanocytic naevi. While AMs are clearly distinguished from CM by displaying few BRAF mutations, they are commonly indistinguishable from CM at the level of gene expression. The aim was to carry out expression analyses of classical immunohistochemical markers and of the protein deleted in malignant brain tumours 1 (DMBT1) in cases of primary anorectal malignant melanoma and CM. METHODS AND RESULTS: Expression analyses of classical immunohistochemical markers (S100, HMB45, Melan A and MiTF) and of the protein DMBT1 were carried out in 27 cases of primary anorectal malignant melanoma and 26 cases of CM. All AM cases analysed showed expression of at least three of the classical markers for melanoma. However, immunohistochemistry showed 19 out of 27 AM to be positive for DMBT1, which represented a statistically significant difference (P = 0.0009) compared with CM (six out of 26), which more commonly are negative for DMBT1 expression. CONCLUSION: These results identify DMBT1 as a molecular feature that may allow distinction between AM and CM and support the notion that AM represents an entity molecularly distinct from CM.
    OriginalsprogEngelsk
    TidsskriftHistopathology
    Vol/bind54
    Udgave nummer2
    Sider (fra-til)233-40
    Antal sider7
    ISSN0309-0167
    DOI
    StatusUdgivet - 2009

    Fingeraftryk

    Melanoma
    Skin
    Pigmented Nevus
    Ultraviolet Rays
    Proteins

    Citer dette

    Helmke, B. M., Renner, M., Poustka, A., Schirmacher, P., Mollenhauer, J., & Kern, M. A. (2009). DMBT1 expression distinguishes anorectal from cutaneous melanoma. Histopathology, 54(2), 233-40. https://doi.org/10.1111/j.1365-2559.2008.03200.x
    Helmke, Burkhard Maria ; Renner, Marcus ; Poustka, Annemarie ; Schirmacher, Peter ; Mollenhauer, Jan ; Kern, Michael André. / DMBT1 expression distinguishes anorectal from cutaneous melanoma. I: Histopathology. 2009 ; Bind 54, Nr. 2. s. 233-40.
    @article{4daf5200af3011de9743000ea68e967b,
    title = "DMBT1 expression distinguishes anorectal from cutaneous melanoma",
    abstract = "AIMS: Anorectal melanoma (AM) forms a rare but highly malignant subset of mucosal melanoma with an extremely poor prognosis. Although AMs display histological and immunohistochemical features very similar to cutaneous melanoma (CM), no association exists either with exposure to ultraviolet light or with melanocytic naevi. While AMs are clearly distinguished from CM by displaying few BRAF mutations, they are commonly indistinguishable from CM at the level of gene expression. The aim was to carry out expression analyses of classical immunohistochemical markers and of the protein deleted in malignant brain tumours 1 (DMBT1) in cases of primary anorectal malignant melanoma and CM. METHODS AND RESULTS: Expression analyses of classical immunohistochemical markers (S100, HMB45, Melan A and MiTF) and of the protein DMBT1 were carried out in 27 cases of primary anorectal malignant melanoma and 26 cases of CM. All AM cases analysed showed expression of at least three of the classical markers for melanoma. However, immunohistochemistry showed 19 out of 27 AM to be positive for DMBT1, which represented a statistically significant difference (P = 0.0009) compared with CM (six out of 26), which more commonly are negative for DMBT1 expression. CONCLUSION: These results identify DMBT1 as a molecular feature that may allow distinction between AM and CM and support the notion that AM represents an entity molecularly distinct from CM.",
    author = "Helmke, {Burkhard Maria} and Marcus Renner and Annemarie Poustka and Peter Schirmacher and Jan Mollenhauer and Kern, {Michael Andr{\'e}}",
    year = "2009",
    doi = "10.1111/j.1365-2559.2008.03200.x",
    language = "English",
    volume = "54",
    pages = "233--40",
    journal = "Histopathology",
    issn = "0309-0167",
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    }

    Helmke, BM, Renner, M, Poustka, A, Schirmacher, P, Mollenhauer, J & Kern, MA 2009, 'DMBT1 expression distinguishes anorectal from cutaneous melanoma', Histopathology, bind 54, nr. 2, s. 233-40. https://doi.org/10.1111/j.1365-2559.2008.03200.x

    DMBT1 expression distinguishes anorectal from cutaneous melanoma. / Helmke, Burkhard Maria; Renner, Marcus; Poustka, Annemarie; Schirmacher, Peter; Mollenhauer, Jan; Kern, Michael André.

    I: Histopathology, Bind 54, Nr. 2, 2009, s. 233-40.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - DMBT1 expression distinguishes anorectal from cutaneous melanoma

    AU - Helmke, Burkhard Maria

    AU - Renner, Marcus

    AU - Poustka, Annemarie

    AU - Schirmacher, Peter

    AU - Mollenhauer, Jan

    AU - Kern, Michael André

    PY - 2009

    Y1 - 2009

    N2 - AIMS: Anorectal melanoma (AM) forms a rare but highly malignant subset of mucosal melanoma with an extremely poor prognosis. Although AMs display histological and immunohistochemical features very similar to cutaneous melanoma (CM), no association exists either with exposure to ultraviolet light or with melanocytic naevi. While AMs are clearly distinguished from CM by displaying few BRAF mutations, they are commonly indistinguishable from CM at the level of gene expression. The aim was to carry out expression analyses of classical immunohistochemical markers and of the protein deleted in malignant brain tumours 1 (DMBT1) in cases of primary anorectal malignant melanoma and CM. METHODS AND RESULTS: Expression analyses of classical immunohistochemical markers (S100, HMB45, Melan A and MiTF) and of the protein DMBT1 were carried out in 27 cases of primary anorectal malignant melanoma and 26 cases of CM. All AM cases analysed showed expression of at least three of the classical markers for melanoma. However, immunohistochemistry showed 19 out of 27 AM to be positive for DMBT1, which represented a statistically significant difference (P = 0.0009) compared with CM (six out of 26), which more commonly are negative for DMBT1 expression. CONCLUSION: These results identify DMBT1 as a molecular feature that may allow distinction between AM and CM and support the notion that AM represents an entity molecularly distinct from CM.

    AB - AIMS: Anorectal melanoma (AM) forms a rare but highly malignant subset of mucosal melanoma with an extremely poor prognosis. Although AMs display histological and immunohistochemical features very similar to cutaneous melanoma (CM), no association exists either with exposure to ultraviolet light or with melanocytic naevi. While AMs are clearly distinguished from CM by displaying few BRAF mutations, they are commonly indistinguishable from CM at the level of gene expression. The aim was to carry out expression analyses of classical immunohistochemical markers and of the protein deleted in malignant brain tumours 1 (DMBT1) in cases of primary anorectal malignant melanoma and CM. METHODS AND RESULTS: Expression analyses of classical immunohistochemical markers (S100, HMB45, Melan A and MiTF) and of the protein DMBT1 were carried out in 27 cases of primary anorectal malignant melanoma and 26 cases of CM. All AM cases analysed showed expression of at least three of the classical markers for melanoma. However, immunohistochemistry showed 19 out of 27 AM to be positive for DMBT1, which represented a statistically significant difference (P = 0.0009) compared with CM (six out of 26), which more commonly are negative for DMBT1 expression. CONCLUSION: These results identify DMBT1 as a molecular feature that may allow distinction between AM and CM and support the notion that AM represents an entity molecularly distinct from CM.

    U2 - 10.1111/j.1365-2559.2008.03200.x

    DO - 10.1111/j.1365-2559.2008.03200.x

    M3 - Journal article

    C2 - 19207948

    VL - 54

    SP - 233

    EP - 240

    JO - Histopathology

    JF - Histopathology

    SN - 0309-0167

    IS - 2

    ER -

    Helmke BM, Renner M, Poustka A, Schirmacher P, Mollenhauer J, Kern MA. DMBT1 expression distinguishes anorectal from cutaneous melanoma. Histopathology. 2009;54(2):233-40. https://doi.org/10.1111/j.1365-2559.2008.03200.x