DMBT1 encodes a protein involved in the immune defense and in epithelial differentiation and is highly unstable in cancer.

J Mollenhauer, S Herbertz, U Holmskov, M Tolnay, I Krebs, A Merlo, H D Schrøder, D Maier, F Breitling, S Wiemann, H J Gröne, A Poustka

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2000-Mar-15
OriginalsprogEngelsk
TidsskriftCancer Research
Vol/bind60
Udgave nummer6
Sider (fra-til)1704-10
ISSN0008-5472
StatusUdgivet - 15. mar. 2000

Fingeraftryk

Neoplasms
Proteins
Scavenger Receptors
Gastrointestinal Neoplasms
Glioblastoma
Tumor Cell Line
Immune System
Protein Isoforms
Epithelium
Macrophages
Rabbits
Messenger RNA

Citer dette

Mollenhauer, J., Herbertz, S., Holmskov, U., Tolnay, M., Krebs, I., Merlo, A., ... Poustka, A. (2000). DMBT1 encodes a protein involved in the immune defense and in epithelial differentiation and is highly unstable in cancer. Cancer Research, 60(6), 1704-10.
Mollenhauer, J ; Herbertz, S ; Holmskov, U ; Tolnay, M ; Krebs, I ; Merlo, A ; Schrøder, H D ; Maier, D ; Breitling, F ; Wiemann, S ; Gröne, H J ; Poustka, A. / DMBT1 encodes a protein involved in the immune defense and in epithelial differentiation and is highly unstable in cancer. I: Cancer Research. 2000 ; Bind 60, Nr. 6. s. 1704-10.
@article{1a54795062d611ddb1a1000ea68e967b,
title = "DMBT1 encodes a protein involved in the immune defense and in epithelial differentiation and is highly unstable in cancer.",
abstract = "The gene deleted in malignant brain tumors 1 (DMBT1) has been proposed as a candidate tumor suppressor for brain, gastrointestinal, and lung cancer. It codes for a protein of unknown function belonging to the superfamily of scavenger receptor cysteine-rich proteins. We aimed at getting insights into the functions of DMBT1 by expression analyses and studies with a monoclonal antibody against the protein. The DMBT1 mRNA is expressed throughout the immune system, and Western blot studies demonstrated that isoforms of DMBT1 are identical to the collectin-binding protein gp-340, a glycoprotein that is involved in the respiratory immune defense. Immunohistochemical analyses revealed that DMBT1 is produced by both tumor-associated macrophages and tumor cells and that it is deregulated in glioblastoma multiforme in comparison to normal brain tissue. Our data further suggest that the proteins CRP-ductin and hensin, both of which have been implicated in epithelial differentiation, are the DMBT1 orthologs in mice and rabbits, respectively. These findings and the spatial and temporal distribution of DMBT1 in fetal and adult epithelia suggest that DMBT1 further plays a role in epithelial development. Rearrangements of DMBT1 were found in 16 of 18 tumor cell lines, and hemizygous deletions were observed in a subset of normal individuals, indicating that the alterations in tumors may be a result of both pre-existing deletions uncovered by a loss of heterozygosity and secondary changes acquired during tumorigenesis. Thus, DMBT1 is a gene that is highly unstable in cancer and encodes for a protein with at least two different functions, one in the immune defense and a second one in epithelial differentiation.",
keywords = "Agglutinins, Brain, Brain Chemistry, Brain Neoplasms, Bronchoalveolar Lavage Fluid, Cell Differentiation, Epithelial Cells, Gene Expression Regulation, HL-60 Cells, Humans, Immune System, Immunohistochemistry, Jurkat Cells, Loss of Heterozygosity, Neoplasms, Polymorphism, Genetic, Protein Isoforms, RNA, Neoplasm, Receptors, Cell Surface, Reverse Transcriptase Polymerase Chain Reaction, Tissue Distribution, Tumor Cells, Cultured, U937 Cells",
author = "J Mollenhauer and S Herbertz and U Holmskov and M Tolnay and I Krebs and A Merlo and Schr{\o}der, {H D} and D Maier and F Breitling and S Wiemann and Gr{\"o}ne, {H J} and A Poustka",
year = "2000",
month = "3",
day = "15",
language = "English",
volume = "60",
pages = "1704--10",
journal = "Cancer Research",
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Mollenhauer, J, Herbertz, S, Holmskov, U, Tolnay, M, Krebs, I, Merlo, A, Schrøder, HD, Maier, D, Breitling, F, Wiemann, S, Gröne, HJ & Poustka, A 2000, 'DMBT1 encodes a protein involved in the immune defense and in epithelial differentiation and is highly unstable in cancer.', Cancer Research, bind 60, nr. 6, s. 1704-10.

DMBT1 encodes a protein involved in the immune defense and in epithelial differentiation and is highly unstable in cancer. / Mollenhauer, J; Herbertz, S; Holmskov, U; Tolnay, M; Krebs, I; Merlo, A; Schrøder, H D; Maier, D; Breitling, F; Wiemann, S; Gröne, H J; Poustka, A.

I: Cancer Research, Bind 60, Nr. 6, 15.03.2000, s. 1704-10.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - DMBT1 encodes a protein involved in the immune defense and in epithelial differentiation and is highly unstable in cancer.

AU - Mollenhauer, J

AU - Herbertz, S

AU - Holmskov, U

AU - Tolnay, M

AU - Krebs, I

AU - Merlo, A

AU - Schrøder, H D

AU - Maier, D

AU - Breitling, F

AU - Wiemann, S

AU - Gröne, H J

AU - Poustka, A

PY - 2000/3/15

Y1 - 2000/3/15

N2 - The gene deleted in malignant brain tumors 1 (DMBT1) has been proposed as a candidate tumor suppressor for brain, gastrointestinal, and lung cancer. It codes for a protein of unknown function belonging to the superfamily of scavenger receptor cysteine-rich proteins. We aimed at getting insights into the functions of DMBT1 by expression analyses and studies with a monoclonal antibody against the protein. The DMBT1 mRNA is expressed throughout the immune system, and Western blot studies demonstrated that isoforms of DMBT1 are identical to the collectin-binding protein gp-340, a glycoprotein that is involved in the respiratory immune defense. Immunohistochemical analyses revealed that DMBT1 is produced by both tumor-associated macrophages and tumor cells and that it is deregulated in glioblastoma multiforme in comparison to normal brain tissue. Our data further suggest that the proteins CRP-ductin and hensin, both of which have been implicated in epithelial differentiation, are the DMBT1 orthologs in mice and rabbits, respectively. These findings and the spatial and temporal distribution of DMBT1 in fetal and adult epithelia suggest that DMBT1 further plays a role in epithelial development. Rearrangements of DMBT1 were found in 16 of 18 tumor cell lines, and hemizygous deletions were observed in a subset of normal individuals, indicating that the alterations in tumors may be a result of both pre-existing deletions uncovered by a loss of heterozygosity and secondary changes acquired during tumorigenesis. Thus, DMBT1 is a gene that is highly unstable in cancer and encodes for a protein with at least two different functions, one in the immune defense and a second one in epithelial differentiation.

AB - The gene deleted in malignant brain tumors 1 (DMBT1) has been proposed as a candidate tumor suppressor for brain, gastrointestinal, and lung cancer. It codes for a protein of unknown function belonging to the superfamily of scavenger receptor cysteine-rich proteins. We aimed at getting insights into the functions of DMBT1 by expression analyses and studies with a monoclonal antibody against the protein. The DMBT1 mRNA is expressed throughout the immune system, and Western blot studies demonstrated that isoforms of DMBT1 are identical to the collectin-binding protein gp-340, a glycoprotein that is involved in the respiratory immune defense. Immunohistochemical analyses revealed that DMBT1 is produced by both tumor-associated macrophages and tumor cells and that it is deregulated in glioblastoma multiforme in comparison to normal brain tissue. Our data further suggest that the proteins CRP-ductin and hensin, both of which have been implicated in epithelial differentiation, are the DMBT1 orthologs in mice and rabbits, respectively. These findings and the spatial and temporal distribution of DMBT1 in fetal and adult epithelia suggest that DMBT1 further plays a role in epithelial development. Rearrangements of DMBT1 were found in 16 of 18 tumor cell lines, and hemizygous deletions were observed in a subset of normal individuals, indicating that the alterations in tumors may be a result of both pre-existing deletions uncovered by a loss of heterozygosity and secondary changes acquired during tumorigenesis. Thus, DMBT1 is a gene that is highly unstable in cancer and encodes for a protein with at least two different functions, one in the immune defense and a second one in epithelial differentiation.

KW - Agglutinins

KW - Brain

KW - Brain Chemistry

KW - Brain Neoplasms

KW - Bronchoalveolar Lavage Fluid

KW - Cell Differentiation

KW - Epithelial Cells

KW - Gene Expression Regulation

KW - HL-60 Cells

KW - Humans

KW - Immune System

KW - Immunohistochemistry

KW - Jurkat Cells

KW - Loss of Heterozygosity

KW - Neoplasms

KW - Polymorphism, Genetic

KW - Protein Isoforms

KW - RNA, Neoplasm

KW - Receptors, Cell Surface

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Tissue Distribution

KW - Tumor Cells, Cultured

KW - U937 Cells

M3 - Journal article

VL - 60

SP - 1704

EP - 1710

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 6

ER -