Diversity of sulfur isotope fractionations by sulfate-reducing prokaryotes

Jan Detmers, Volker Brüchert, K S Habicht, Jan Kuever

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2001-Feb
OriginalsprogEngelsk
TidsskriftApplied and Environmental Microbiology
Vol/bind67
Udgave nummer2
Sider (fra-til)888-894
Antal sider6
ISSN0099-2240
DOI
StatusUdgivet - 1. feb. 2001

Fingeraftryk

isotope fractionation
prokaryote
sulfur isotope
prokaryotic cells
sulfates
sulfur
fractionation
sulfate
Isotopes
isotope
salinity
bisulfites
Batch Cell Culture Techniques
carbon
phylogeny
Phylogeny
Metabolic Networks and Pathways
Fresh Water
physiology
Ecosystem

Citer dette

Detmers, Jan ; Brüchert, Volker ; Habicht, K S ; Kuever, Jan. / Diversity of sulfur isotope fractionations by sulfate-reducing prokaryotes. I: Applied and Environmental Microbiology. 2001 ; Bind 67, Nr. 2. s. 888-894.
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title = "Diversity of sulfur isotope fractionations by sulfate-reducing prokaryotes",
abstract = "Batch culture experiments were performed with 32 different sulfate-reducing prokaryotes to explore the diversity in sulfur isotope fractionation during dissimilatory sulfate reduction by pure cultures. The selected strains reflect the phylogenetic and physiologic diversity of presently known sulfate reducers and cover a broad range of natural marine and freshwater habitats. Experimental conditions were designed to achieve optimum growth conditions with respect to electron donors, salinity, temperature, and pH. Under these optimized conditions, experimental fractionation factors ranged from 2.0 to 42.0 per thousand. Salinity, incubation temperature, pH, and phylogeny had no systematic effect on the sulfur isotope fractionation. There was no correlation between isotope fractionation and sulfate reduction rate. The type of dissimilatory bisulfite reductase also had no effect on fractionation. Sulfate reducers that oxidized the carbon source completely to CO2 showed greater fractionations than sulfate reducers that released acetate as the final product of carbon oxidation. Different metabolic pathways and variable regulation of sulfate transport across the cell membrane all potentially affect isotope fractionation. Previous models that explained fractionation only in terms of sulfate reduction rates appear to be oversimplified. The species-specific physiology of each sulfate reducer thus needs to be taken into account to understand the regulation of sulfur isotope fractionation during dissimilatory sulfate reduction.",
keywords = "Culture Media, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Sulfates, Sulfides, Sulfur Isotopes, Sulfur-Reducing Bacteria, Water Microbiology",
author = "Jan Detmers and Volker Br{\"u}chert and Habicht, {K S} and Jan Kuever",
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Diversity of sulfur isotope fractionations by sulfate-reducing prokaryotes. / Detmers, Jan; Brüchert, Volker; Habicht, K S; Kuever, Jan.

I: Applied and Environmental Microbiology, Bind 67, Nr. 2, 01.02.2001, s. 888-894.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Diversity of sulfur isotope fractionations by sulfate-reducing prokaryotes

AU - Detmers, Jan

AU - Brüchert, Volker

AU - Habicht, K S

AU - Kuever, Jan

PY - 2001/2/1

Y1 - 2001/2/1

N2 - Batch culture experiments were performed with 32 different sulfate-reducing prokaryotes to explore the diversity in sulfur isotope fractionation during dissimilatory sulfate reduction by pure cultures. The selected strains reflect the phylogenetic and physiologic diversity of presently known sulfate reducers and cover a broad range of natural marine and freshwater habitats. Experimental conditions were designed to achieve optimum growth conditions with respect to electron donors, salinity, temperature, and pH. Under these optimized conditions, experimental fractionation factors ranged from 2.0 to 42.0 per thousand. Salinity, incubation temperature, pH, and phylogeny had no systematic effect on the sulfur isotope fractionation. There was no correlation between isotope fractionation and sulfate reduction rate. The type of dissimilatory bisulfite reductase also had no effect on fractionation. Sulfate reducers that oxidized the carbon source completely to CO2 showed greater fractionations than sulfate reducers that released acetate as the final product of carbon oxidation. Different metabolic pathways and variable regulation of sulfate transport across the cell membrane all potentially affect isotope fractionation. Previous models that explained fractionation only in terms of sulfate reduction rates appear to be oversimplified. The species-specific physiology of each sulfate reducer thus needs to be taken into account to understand the regulation of sulfur isotope fractionation during dissimilatory sulfate reduction.

AB - Batch culture experiments were performed with 32 different sulfate-reducing prokaryotes to explore the diversity in sulfur isotope fractionation during dissimilatory sulfate reduction by pure cultures. The selected strains reflect the phylogenetic and physiologic diversity of presently known sulfate reducers and cover a broad range of natural marine and freshwater habitats. Experimental conditions were designed to achieve optimum growth conditions with respect to electron donors, salinity, temperature, and pH. Under these optimized conditions, experimental fractionation factors ranged from 2.0 to 42.0 per thousand. Salinity, incubation temperature, pH, and phylogeny had no systematic effect on the sulfur isotope fractionation. There was no correlation between isotope fractionation and sulfate reduction rate. The type of dissimilatory bisulfite reductase also had no effect on fractionation. Sulfate reducers that oxidized the carbon source completely to CO2 showed greater fractionations than sulfate reducers that released acetate as the final product of carbon oxidation. Different metabolic pathways and variable regulation of sulfate transport across the cell membrane all potentially affect isotope fractionation. Previous models that explained fractionation only in terms of sulfate reduction rates appear to be oversimplified. The species-specific physiology of each sulfate reducer thus needs to be taken into account to understand the regulation of sulfur isotope fractionation during dissimilatory sulfate reduction.

KW - Culture Media

KW - Genes, rRNA

KW - Molecular Sequence Data

KW - Phylogeny

KW - RNA, Ribosomal, 16S

KW - Sequence Analysis, DNA

KW - Sulfates

KW - Sulfides

KW - Sulfur Isotopes

KW - Sulfur-Reducing Bacteria

KW - Water Microbiology

U2 - 10.1128/AEM.67.2.888-894.2001

DO - 10.1128/AEM.67.2.888-894.2001

M3 - Journal article

VL - 67

SP - 888

EP - 894

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 2

ER -