Distinguishing of Ile and Leu residues in the PP3 protein by (hot) electron capture dissociation

Frank Kjeldsen; Roman A. Zubarev

Distinguishing of Ile and Leu residues in the PP3 protein by (hot) electron capture dissociation

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Publikation: Konferencebidrag uden forlag/tidsskrift › Paper › Forskning › peer review

Abstrakt

Hot Electron Capture Dissociation (HECD) was used for the distinguishing of isomeric residues lle and Leu for polypeptide sequencing. HECD was implemented on a 4.7 Tesla Ultima IonSpec Fourier transform (FT) instrument and a indirectly heated dispenser cathode was used as a source of electrons. HECD utilized electrons with substantially higher energy and the excess energy in HECD lead to secondary cleavages including w ions. The results show that the characteristic loss of CH(CH₃)₂ from Leu and CH₂CH₃ from lle that lead to w ions and distinguish the two amino acids are observed in 20 cases of the total 25 Xle residues.

Originalsprog	Engelsk
Publikationsdato	1. dec. 2002
Antal sider	2
Status	Udgivet - 1. dec. 2002
Begivenhed	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, USA Varighed: 2. jun. 2002 → 6. jun. 2002

Konference

Konference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Land	USA
By	Orlando, FL
Periode	02/06/2002 → 06/06/2002
Sponsor	American Society for Mass Spectrometry (ASMS)

Dokumenter og links

Link to publication in Scopus

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Citationsformater

@conference{5ca1e1e1960d4a8f9988a74b93df0367,

title = "Distinguishing of Ile and Leu residues in the PP3 protein by (hot) electron capture dissociation",

abstract = "Hot Electron Capture Dissociation (HECD) was used for the distinguishing of isomeric residues lle and Leu for polypeptide sequencing. HECD was implemented on a 4.7 Tesla Ultima IonSpec Fourier transform (FT) instrument and a indirectly heated dispenser cathode was used as a source of electrons. HECD utilized electrons with substantially higher energy and the excess energy in HECD lead to secondary cleavages including w ions. The results show that the characteristic loss of CH(CH3)2 from Leu and CH2CH3 from lle that lead to w ions and distinguish the two amino acids are observed in 20 cases of the total 25 Xle residues.",

author = "Frank Kjeldsen and Zubarev, {Roman A.}",

year = "2002",

month = dec,

day = "1",

language = "English",

pages = "103--104",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Distinguishing of Ile and Leu residues in the PP3 protein by (hot) electron capture dissociation

AU - Kjeldsen, Frank

AU - Zubarev, Roman A.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Hot Electron Capture Dissociation (HECD) was used for the distinguishing of isomeric residues lle and Leu for polypeptide sequencing. HECD was implemented on a 4.7 Tesla Ultima IonSpec Fourier transform (FT) instrument and a indirectly heated dispenser cathode was used as a source of electrons. HECD utilized electrons with substantially higher energy and the excess energy in HECD lead to secondary cleavages including w ions. The results show that the characteristic loss of CH(CH3)2 from Leu and CH2CH3 from lle that lead to w ions and distinguish the two amino acids are observed in 20 cases of the total 25 Xle residues.

AB - Hot Electron Capture Dissociation (HECD) was used for the distinguishing of isomeric residues lle and Leu for polypeptide sequencing. HECD was implemented on a 4.7 Tesla Ultima IonSpec Fourier transform (FT) instrument and a indirectly heated dispenser cathode was used as a source of electrons. HECD utilized electrons with substantially higher energy and the excess energy in HECD lead to secondary cleavages including w ions. The results show that the characteristic loss of CH(CH3)2 from Leu and CH2CH3 from lle that lead to w ions and distinguish the two amino acids are observed in 20 cases of the total 25 Xle residues.

UR - http://www.scopus.com/inward/record.url?scp=2442639052&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442639052

SP - 103

EP - 104

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -