Hot Electron Capture Dissociation (HECD) was used for the distinguishing of isomeric residues lle and Leu for polypeptide sequencing. HECD was implemented on a 4.7 Tesla Ultima IonSpec Fourier transform (FT) instrument and a indirectly heated dispenser cathode was used as a source of electrons. HECD utilized electrons with substantially higher energy and the excess energy in HECD lead to secondary cleavages including w ions. The results show that the characteristic loss of CH(CH3)2 from Leu and CH2CH3 from lle that lead to w ions and distinguish the two amino acids are observed in 20 cases of the total 25 Xle residues.
|Publikationsdato||1. dec. 2002|
|Status||Udgivet - 1. dec. 2002|
|Begivenhed||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, USA|
Varighed: 2. jun. 2002 → 6. jun. 2002
|Konference||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics|
|Periode||02/06/2002 → 06/06/2002|
|Sponsor||American Society for Mass Spectrometry (ASMS)|