Distinguishing of Ile and Leu residues in the PP3 protein by (hot) electron capture dissociation

Frank Kjeldsen*, Roman A. Zubarev

*Kontaktforfatter for dette arbejde

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Abstrakt

Hot Electron Capture Dissociation (HECD) was used for the distinguishing of isomeric residues lle and Leu for polypeptide sequencing. HECD was implemented on a 4.7 Tesla Ultima IonSpec Fourier transform (FT) instrument and a indirectly heated dispenser cathode was used as a source of electrons. HECD utilized electrons with substantially higher energy and the excess energy in HECD lead to secondary cleavages including w ions. The results show that the characteristic loss of CH(CH3)2 from Leu and CH2CH3 from lle that lead to w ions and distinguish the two amino acids are observed in 20 cases of the total 25 Xle residues.

OriginalsprogEngelsk
Publikationsdato1. dec. 2002
Antal sider2
StatusUdgivet - 1. dec. 2002
BegivenhedProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, USA
Varighed: 2. jun. 20026. jun. 2002

Konference

KonferenceProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
LandUSA
ByOrlando, FL
Periode02/06/200206/06/2002
SponsorAmerican Society for Mass Spectrometry (ASMS)

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