Direct evidence of fiber type-dependent GLUT-4 expression in human skeletal muscle.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2000-May
OriginalsprogEngelsk
TidsskriftAmerican Journal of Physiology: Endocrinology and Metabolism
Vol/bind278
Udgave nummer5
Sider (fra-til)E910-6
ISSN0193-1849
StatusUdgivet - 1. maj 2000

Fingeraftryk

Skeletal Muscle
Muscles
Young Adult
Insulin

Citer dette

@article{09f6691062d611ddb1a1000ea68e967b,
title = "Direct evidence of fiber type-dependent GLUT-4 expression in human skeletal muscle.",
abstract = "GLUT-4 expression in individual fibers of human skeletal muscles in younger and older adults was studied. Furthermore, the dependency of insulin-stimulated glucose uptake on fiber type distribution was investigated. Fiber type distribution was determined in cryosections of muscle biopsies from 8 younger (29 yr) and 8 older (64 yr) healthy subjects, and estimates of GLUT-4 expression in individual fibers were obtained by combining immunohistochemistry and stereology. GLUT-4 was more abundantly expressed in slow compared with fast muscle fibers in both younger (P < 0.007) and older (P < 0. 001) subjects. A 25{\%} reduction of GLUT-4 density in fast fibers (P < 0.001) and an unchanged GLUT-4 density in slow fibers were demonstrated in older compared with younger subjects. Insulin-stimulated glucose uptake rates measured by hyperinsulinemic, euglycemic clamp were not correlated with the fraction of slow fibers in the young (r = -0.45, P > 0.25) or in the elderly (r = 0. 11, P > 0.75) subjects. In conclusion, in human skeletal muscle, GLUT-4 expression is fiber type dependent and decreases with age, particularly in fast muscle fibers.",
keywords = "Adult, Aging, Biopsy, Blotting, Western, Female, Glucose, Glucose Clamp Technique, Glucose Tolerance Test, Glucose Transporter Type 4, Humans, Immunohistochemistry, Insulin, Male, Middle Aged, Monosaccharide Transport Proteins, Muscle Fibers, Fast-Twitch, Muscle Fibers, Slow-Twitch, Muscle Proteins, Muscle, Skeletal",
author = "M Gaster and P Poulsen and A Handberg and H Beck-Nielsen and Schr{\o}der, {Henrik Daa}",
year = "2000",
month = "5",
day = "1",
language = "English",
volume = "278",
pages = "E910--6",
journal = "American Journal of Physiology: Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "5",

}

Direct evidence of fiber type-dependent GLUT-4 expression in human skeletal muscle. / Gaster, M; Poulsen, P; Handberg, A; Beck-Nielsen, H; Schrøder, Henrik Daa.

I: American Journal of Physiology: Endocrinology and Metabolism, Bind 278, Nr. 5, 01.05.2000, s. E910-6.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Direct evidence of fiber type-dependent GLUT-4 expression in human skeletal muscle.

AU - Gaster, M

AU - Poulsen, P

AU - Handberg, A

AU - Beck-Nielsen, H

AU - Schrøder, Henrik Daa

PY - 2000/5/1

Y1 - 2000/5/1

N2 - GLUT-4 expression in individual fibers of human skeletal muscles in younger and older adults was studied. Furthermore, the dependency of insulin-stimulated glucose uptake on fiber type distribution was investigated. Fiber type distribution was determined in cryosections of muscle biopsies from 8 younger (29 yr) and 8 older (64 yr) healthy subjects, and estimates of GLUT-4 expression in individual fibers were obtained by combining immunohistochemistry and stereology. GLUT-4 was more abundantly expressed in slow compared with fast muscle fibers in both younger (P < 0.007) and older (P < 0. 001) subjects. A 25% reduction of GLUT-4 density in fast fibers (P < 0.001) and an unchanged GLUT-4 density in slow fibers were demonstrated in older compared with younger subjects. Insulin-stimulated glucose uptake rates measured by hyperinsulinemic, euglycemic clamp were not correlated with the fraction of slow fibers in the young (r = -0.45, P > 0.25) or in the elderly (r = 0. 11, P > 0.75) subjects. In conclusion, in human skeletal muscle, GLUT-4 expression is fiber type dependent and decreases with age, particularly in fast muscle fibers.

AB - GLUT-4 expression in individual fibers of human skeletal muscles in younger and older adults was studied. Furthermore, the dependency of insulin-stimulated glucose uptake on fiber type distribution was investigated. Fiber type distribution was determined in cryosections of muscle biopsies from 8 younger (29 yr) and 8 older (64 yr) healthy subjects, and estimates of GLUT-4 expression in individual fibers were obtained by combining immunohistochemistry and stereology. GLUT-4 was more abundantly expressed in slow compared with fast muscle fibers in both younger (P < 0.007) and older (P < 0. 001) subjects. A 25% reduction of GLUT-4 density in fast fibers (P < 0.001) and an unchanged GLUT-4 density in slow fibers were demonstrated in older compared with younger subjects. Insulin-stimulated glucose uptake rates measured by hyperinsulinemic, euglycemic clamp were not correlated with the fraction of slow fibers in the young (r = -0.45, P > 0.25) or in the elderly (r = 0. 11, P > 0.75) subjects. In conclusion, in human skeletal muscle, GLUT-4 expression is fiber type dependent and decreases with age, particularly in fast muscle fibers.

KW - Adult

KW - Aging

KW - Biopsy

KW - Blotting, Western

KW - Female

KW - Glucose

KW - Glucose Clamp Technique

KW - Glucose Tolerance Test

KW - Glucose Transporter Type 4

KW - Humans

KW - Immunohistochemistry

KW - Insulin

KW - Male

KW - Middle Aged

KW - Monosaccharide Transport Proteins

KW - Muscle Fibers, Fast-Twitch

KW - Muscle Fibers, Slow-Twitch

KW - Muscle Proteins

KW - Muscle, Skeletal

M3 - Journal article

VL - 278

SP - E910-6

JO - American Journal of Physiology: Endocrinology and Metabolism

JF - American Journal of Physiology: Endocrinology and Metabolism

SN - 0193-1849

IS - 5

ER -