Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones

Steffen Madsen, Lars Nørholm Jensen, Christian Kølbaek Tipsmark, Pia Kiilerich, Russell John Borski

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

 
Udgivelsesdato: Januar
OriginalsprogEngelsk
TidsskriftJournal of Endocrinology
Vol/bind192
Udgave nummer1
Sider (fra-til)249-60
Antal sider11
ISSN0022-0795
DOI
StatusUdgivet - 1. jan. 2007

Fingeraftryk

Cystic Fibrosis Transmembrane Conductance Regulator
Hormones
Epidermal Growth Factor
Messenger RNA
Seawater
Fresh Water
Insulin-Like Growth Factor I
sodium-translocating ATPase
Serum
Fishes
Proteins
Salts
Polymerase Chain Reaction

Citer dette

@article{f2dd21c0d64811dc860c000ea68e967b,
title = "Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones",
abstract = "Effects of salinity and hormones on cystic fibrosis transmembrane conductance regulator (CFTR) and alpha-subunit Na(+),K(+) -ATPase (alpha-NKA) mRNA (analysed by semi-quantitative PCR) and protein expression (analysed by western blotting and immunocytochemistry) were investigated in gills of striped bass. Freshwater (FW) to seawater (SW) transfer induced a disturbance in serum [Na(+)]. Gill CFTR protein, mRNA level and Na(+),K(+) -ATPase activity were unaffected by SW transfer, whereas alpha-NKA mRNA increased after transfer. CFTR immunoreactivity was observed in large cells in FW and SW gill filaments at equal intensity. Cortisol decreased serum [Na(+)] in FW fish, but had no effect on gill Na(+),K(+) -ATPase activity, alpha-NKA and CFTR mRNA levels. Incubation of gill tissue with cortisol (24 h, >0.01 micro g/ml) and epidermal growth factor (EGF 10 micro g/ml) decreased CFTR mRNA levels relative to pre-incubation and control levels. CFTR expression was unaffected by IGF-I (10 micro g/ml). alpha-NKA mRNA levels decreased by 50{\%} after 24 h control incubation; it was slightly stimulated by cortisol and unaffected by IGF-I and EGF. In isolated gill cells, phosphorylation of extracellular-regulated kinase (ERK) 1/2 was stimulated by EGF but not affected by IGF-I. This study is the first to report a branchial EGF response and to demonstrate a functional ERK 1/2 pathway in the teleost gill. In conclusion, CFTR and Na(+),K(+) -ATPase are differentially regulated by salinity and hormones in gills of striped bass, despite the putative involvement of both in salt excretion.",
keywords = "Animals, Base Sequence, Bass, Blotting, Western, Cystic Fibrosis Transmembrane Conductance Regulator, DNA Primers, Epidermal Growth Factor, Fresh Water, Gills, Hydrocortisone, Immunohistochemistry, Insulin-Like Growth Factor I, Mitogen-Activated Protein Kinase Kinases, Molecular Sequence Data, Osmolar Concentration, Protein Subunits, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Seawater, Sodium, Sodium-Potassium-Exchanging ATPase, Water-Electrolyte Balance",
author = "Steffen Madsen and Jensen, {Lars N{\o}rholm} and Tipsmark, {Christian K{\o}lbaek} and Pia Kiilerich and Borski, {Russell John}",
year = "2007",
month = "1",
day = "1",
doi = "10.1677/JOE-06-0016",
language = "English",
volume = "192",
pages = "249--60",
journal = "Journal of Endocrinology",
issn = "0022-0795",
publisher = "BioScientifica Ltd.",
number = "1",

}

Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones. / Madsen, Steffen; Jensen, Lars Nørholm; Tipsmark, Christian Kølbaek; Kiilerich, Pia; Borski, Russell John.

I: Journal of Endocrinology, Bind 192, Nr. 1, 01.01.2007, s. 249-60.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones

AU - Madsen, Steffen

AU - Jensen, Lars Nørholm

AU - Tipsmark, Christian Kølbaek

AU - Kiilerich, Pia

AU - Borski, Russell John

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Effects of salinity and hormones on cystic fibrosis transmembrane conductance regulator (CFTR) and alpha-subunit Na(+),K(+) -ATPase (alpha-NKA) mRNA (analysed by semi-quantitative PCR) and protein expression (analysed by western blotting and immunocytochemistry) were investigated in gills of striped bass. Freshwater (FW) to seawater (SW) transfer induced a disturbance in serum [Na(+)]. Gill CFTR protein, mRNA level and Na(+),K(+) -ATPase activity were unaffected by SW transfer, whereas alpha-NKA mRNA increased after transfer. CFTR immunoreactivity was observed in large cells in FW and SW gill filaments at equal intensity. Cortisol decreased serum [Na(+)] in FW fish, but had no effect on gill Na(+),K(+) -ATPase activity, alpha-NKA and CFTR mRNA levels. Incubation of gill tissue with cortisol (24 h, >0.01 micro g/ml) and epidermal growth factor (EGF 10 micro g/ml) decreased CFTR mRNA levels relative to pre-incubation and control levels. CFTR expression was unaffected by IGF-I (10 micro g/ml). alpha-NKA mRNA levels decreased by 50% after 24 h control incubation; it was slightly stimulated by cortisol and unaffected by IGF-I and EGF. In isolated gill cells, phosphorylation of extracellular-regulated kinase (ERK) 1/2 was stimulated by EGF but not affected by IGF-I. This study is the first to report a branchial EGF response and to demonstrate a functional ERK 1/2 pathway in the teleost gill. In conclusion, CFTR and Na(+),K(+) -ATPase are differentially regulated by salinity and hormones in gills of striped bass, despite the putative involvement of both in salt excretion.

AB - Effects of salinity and hormones on cystic fibrosis transmembrane conductance regulator (CFTR) and alpha-subunit Na(+),K(+) -ATPase (alpha-NKA) mRNA (analysed by semi-quantitative PCR) and protein expression (analysed by western blotting and immunocytochemistry) were investigated in gills of striped bass. Freshwater (FW) to seawater (SW) transfer induced a disturbance in serum [Na(+)]. Gill CFTR protein, mRNA level and Na(+),K(+) -ATPase activity were unaffected by SW transfer, whereas alpha-NKA mRNA increased after transfer. CFTR immunoreactivity was observed in large cells in FW and SW gill filaments at equal intensity. Cortisol decreased serum [Na(+)] in FW fish, but had no effect on gill Na(+),K(+) -ATPase activity, alpha-NKA and CFTR mRNA levels. Incubation of gill tissue with cortisol (24 h, >0.01 micro g/ml) and epidermal growth factor (EGF 10 micro g/ml) decreased CFTR mRNA levels relative to pre-incubation and control levels. CFTR expression was unaffected by IGF-I (10 micro g/ml). alpha-NKA mRNA levels decreased by 50% after 24 h control incubation; it was slightly stimulated by cortisol and unaffected by IGF-I and EGF. In isolated gill cells, phosphorylation of extracellular-regulated kinase (ERK) 1/2 was stimulated by EGF but not affected by IGF-I. This study is the first to report a branchial EGF response and to demonstrate a functional ERK 1/2 pathway in the teleost gill. In conclusion, CFTR and Na(+),K(+) -ATPase are differentially regulated by salinity and hormones in gills of striped bass, despite the putative involvement of both in salt excretion.

KW - Animals

KW - Base Sequence

KW - Bass

KW - Blotting, Western

KW - Cystic Fibrosis Transmembrane Conductance Regulator

KW - DNA Primers

KW - Epidermal Growth Factor

KW - Fresh Water

KW - Gills

KW - Hydrocortisone

KW - Immunohistochemistry

KW - Insulin-Like Growth Factor I

KW - Mitogen-Activated Protein Kinase Kinases

KW - Molecular Sequence Data

KW - Osmolar Concentration

KW - Protein Subunits

KW - RNA, Messenger

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Seawater

KW - Sodium

KW - Sodium-Potassium-Exchanging ATPase

KW - Water-Electrolyte Balance

U2 - 10.1677/JOE-06-0016

DO - 10.1677/JOE-06-0016

M3 - Journal article

C2 - 17210762

VL - 192

SP - 249

EP - 260

JO - Journal of Endocrinology

JF - Journal of Endocrinology

SN - 0022-0795

IS - 1

ER -