Differential expression of monocyte CD163 in single- and dual-asthmatic responders during allergen-induced bronchoconstriction

K Kowal; H J Møller; L M Dubuske; S K Moestrup; A Bodzenta-Lukaszyk

doi:10.1111/j.1365-2222.2006.02573.x

Differential expression of monocyte CD163 in single- and dual-asthmatic responders during allergen-induced bronchoconstriction

K Kowal, H J Møller, L M Dubuske, S K Moestrup, A Bodzenta-Lukaszyk

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

BACKGROUND: Mononuclear phagocytes play an important role in modulating inflammatory reactions in response to antigen challenge.

OBJECTIVE: To investigate the regulation of CD163, a marker of anti-inflammatory macrophages, during Dermatophagoides pteronyssinus (Dp)-induced bronchoconstriction.

METHODS: Among 110 Dp-sensitive patients who underwent bronchial challenge with Dp, there were 51 dual responders (DR), 32 single responders (SR) and 27 non-responders (NR). Monocyte expression of CD14 and CD163 was evaluated by flow cytometry. Exhaled NO (eNO) concentration was determined on-line using a chemiluminescence analyser. In 21 DR, nine SR and 13 NR-soluble CD163 in plasma was measured by ELISA before, 1, 8 and 24 h after the challenge.

RESULTS: In DR, the baseline expression of monocyte CD163 and eNO were significantly greater than in SR and NR. A pattern of (1) decreased monocyte CD163 expression, (2) unchanged sCD163 and (3) increased eNO in DR was contrasted by a pattern of (1) increased CD163 expression, (2) increased sCD163 and (3) unchanged eNO in SR. During allergen challenge, the changes in monocyte CD163 expression and sCD163 inversely correlated with the changes in eNO.

CONCLUSION: Both pro-inflammatory and anti-inflammatory responses to allergen challenge are uniquely expressed among SR and DR.

Originalsprog	Engelsk
Tidsskrift	Clinical and Experimental Allergy
Vol/bind	36
Udgave nummer	12
Sider (fra-til)	1584-91
Antal sider	8
ISSN	0954-7894
DOI	https://doi.org/10.1111/j.1365-2222.2006.02573.x
Status	Udgivet - dec. 2006

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10.1111/j.1365-2222.2006.02573.x

Citationsformater

@article{e48c7af6a33548f1931b1f97829f9011,

title = "Differential expression of monocyte CD163 in single- and dual-asthmatic responders during allergen-induced bronchoconstriction",

abstract = "BACKGROUND: Mononuclear phagocytes play an important role in modulating inflammatory reactions in response to antigen challenge.OBJECTIVE: To investigate the regulation of CD163, a marker of anti-inflammatory macrophages, during Dermatophagoides pteronyssinus (Dp)-induced bronchoconstriction.METHODS: Among 110 Dp-sensitive patients who underwent bronchial challenge with Dp, there were 51 dual responders (DR), 32 single responders (SR) and 27 non-responders (NR). Monocyte expression of CD14 and CD163 was evaluated by flow cytometry. Exhaled NO (eNO) concentration was determined on-line using a chemiluminescence analyser. In 21 DR, nine SR and 13 NR-soluble CD163 in plasma was measured by ELISA before, 1, 8 and 24 h after the challenge.RESULTS: In DR, the baseline expression of monocyte CD163 and eNO were significantly greater than in SR and NR. A pattern of (1) decreased monocyte CD163 expression, (2) unchanged sCD163 and (3) increased eNO in DR was contrasted by a pattern of (1) increased CD163 expression, (2) increased sCD163 and (3) unchanged eNO in SR. During allergen challenge, the changes in monocyte CD163 expression and sCD163 inversely correlated with the changes in eNO.CONCLUSION: Both pro-inflammatory and anti-inflammatory responses to allergen challenge are uniquely expressed among SR and DR.",

keywords = "Adolescent, Adult, Analysis of Variance, Animals, Antigens, CD, Antigens, CD14, Antigens, Dermatophagoides, Antigens, Differentiation, Myelomonocytic, Biological Markers, Breath Tests, Bronchial Provocation Tests, Dermatophagoides pteronyssinus, Dust, Female, Flow Cytometry, Humans, Immunologic Tests, Lung, Macrophages, Male, Nitric Oxide, Receptors, Cell Surface, Rhinitis, Allergic, Perennial",

author = "K Kowal and M{\o}ller, {H J} and Dubuske, {L M} and Moestrup, {S K} and A Bodzenta-Lukaszyk",

year = "2006",

month = dec,

doi = "10.1111/j.1365-2222.2006.02573.x",

language = "English",

volume = "36",

pages = "1584--91",

journal = "Clinical and Experimental Allergy",

issn = "0954-7894",

publisher = "Wiley",

number = "12",

}

TY - JOUR

T1 - Differential expression of monocyte CD163 in single- and dual-asthmatic responders during allergen-induced bronchoconstriction

AU - Kowal, K

AU - Møller, H J

AU - Dubuske, L M

AU - Moestrup, S K

AU - Bodzenta-Lukaszyk, A

PY - 2006/12

Y1 - 2006/12

N2 - BACKGROUND: Mononuclear phagocytes play an important role in modulating inflammatory reactions in response to antigen challenge.OBJECTIVE: To investigate the regulation of CD163, a marker of anti-inflammatory macrophages, during Dermatophagoides pteronyssinus (Dp)-induced bronchoconstriction.METHODS: Among 110 Dp-sensitive patients who underwent bronchial challenge with Dp, there were 51 dual responders (DR), 32 single responders (SR) and 27 non-responders (NR). Monocyte expression of CD14 and CD163 was evaluated by flow cytometry. Exhaled NO (eNO) concentration was determined on-line using a chemiluminescence analyser. In 21 DR, nine SR and 13 NR-soluble CD163 in plasma was measured by ELISA before, 1, 8 and 24 h after the challenge.RESULTS: In DR, the baseline expression of monocyte CD163 and eNO were significantly greater than in SR and NR. A pattern of (1) decreased monocyte CD163 expression, (2) unchanged sCD163 and (3) increased eNO in DR was contrasted by a pattern of (1) increased CD163 expression, (2) increased sCD163 and (3) unchanged eNO in SR. During allergen challenge, the changes in monocyte CD163 expression and sCD163 inversely correlated with the changes in eNO.CONCLUSION: Both pro-inflammatory and anti-inflammatory responses to allergen challenge are uniquely expressed among SR and DR.

AB - BACKGROUND: Mononuclear phagocytes play an important role in modulating inflammatory reactions in response to antigen challenge.OBJECTIVE: To investigate the regulation of CD163, a marker of anti-inflammatory macrophages, during Dermatophagoides pteronyssinus (Dp)-induced bronchoconstriction.METHODS: Among 110 Dp-sensitive patients who underwent bronchial challenge with Dp, there were 51 dual responders (DR), 32 single responders (SR) and 27 non-responders (NR). Monocyte expression of CD14 and CD163 was evaluated by flow cytometry. Exhaled NO (eNO) concentration was determined on-line using a chemiluminescence analyser. In 21 DR, nine SR and 13 NR-soluble CD163 in plasma was measured by ELISA before, 1, 8 and 24 h after the challenge.RESULTS: In DR, the baseline expression of monocyte CD163 and eNO were significantly greater than in SR and NR. A pattern of (1) decreased monocyte CD163 expression, (2) unchanged sCD163 and (3) increased eNO in DR was contrasted by a pattern of (1) increased CD163 expression, (2) increased sCD163 and (3) unchanged eNO in SR. During allergen challenge, the changes in monocyte CD163 expression and sCD163 inversely correlated with the changes in eNO.CONCLUSION: Both pro-inflammatory and anti-inflammatory responses to allergen challenge are uniquely expressed among SR and DR.

KW - Adolescent

KW - Adult

KW - Analysis of Variance

KW - Animals

KW - Antigens, CD

KW - Antigens, CD14

KW - Antigens, Dermatophagoides

KW - Antigens, Differentiation, Myelomonocytic

KW - Biological Markers

KW - Breath Tests

KW - Bronchial Provocation Tests

KW - Dermatophagoides pteronyssinus

KW - Dust

KW - Female

KW - Flow Cytometry

KW - Humans

KW - Immunologic Tests

KW - Lung

KW - Macrophages

KW - Male

KW - Nitric Oxide

KW - Receptors, Cell Surface

KW - Rhinitis, Allergic, Perennial

U2 - 10.1111/j.1365-2222.2006.02573.x

DO - 10.1111/j.1365-2222.2006.02573.x

M3 - Journal article

C2 - 17177682

SN - 0954-7894

VL - 36

SP - 1584

EP - 1591

JO - Clinical and Experimental Allergy

JF - Clinical and Experimental Allergy

IS - 12

ER -

Differential expression of monocyte CD163 in single- and dual-asthmatic responders during allergen-induced bronchoconstriction

Abstract

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