Differential effect of extracellular calcium on the Na(+)-K+ pump activity in intact polymorphonuclear leucocytes and erythrocytes

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Resumé

The effect of extracellular calcium on the Na(+)-K+ pump activity in human polymorphonuclear leucocytes and erythrocytes was studied and compared with the activity in mixed peritoneal leucocytes from rats. While there was maximal decrease in the pump activity (25-30%) of leucocytes from both rat and human by calcium 0.6 mM, a concentration of 0.1 mM caused a substantial decrease indicating a high sensitivity for extracellular calcium. In contrast, calcium had no effect on the pump activity in erythrocytes. The effect of calcium on the pump activity in leucocytes may be due to regulation of the influx of sodium across the plasma membrane, since in human leucocytes calcium had no effect on the pump activity if the cells were loaded with sodium.
OriginalsprogEngelsk
TidsskriftF E B S Letters
Vol/bind279
Udgave nummer1
Sider (fra-til)153-6
Antal sider4
ISSN0014-5793
StatusUdgivet - 1991

Fingeraftryk

Neutrophils
Pumps
Calcium
Rats
Sodium
Cell membranes
Human Activities
Cell Membrane

Citer dette

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title = "Differential effect of extracellular calcium on the Na(+)-K+ pump activity in intact polymorphonuclear leucocytes and erythrocytes",
abstract = "The effect of extracellular calcium on the Na(+)-K+ pump activity in human polymorphonuclear leucocytes and erythrocytes was studied and compared with the activity in mixed peritoneal leucocytes from rats. While there was maximal decrease in the pump activity (25-30{\%}) of leucocytes from both rat and human by calcium 0.6 mM, a concentration of 0.1 mM caused a substantial decrease indicating a high sensitivity for extracellular calcium. In contrast, calcium had no effect on the pump activity in erythrocytes. The effect of calcium on the pump activity in leucocytes may be due to regulation of the influx of sodium across the plasma membrane, since in human leucocytes calcium had no effect on the pump activity if the cells were loaded with sodium.",
keywords = "Animals, Calcium, Carrier Proteins, Erythrocytes, Humans, Male, Membrane Proteins, Neutrophils, Potassium, Rats, Rats, Inbred Strains, Sodium, Sodium-Potassium-Chloride Symporters",
author = "Petersen, {R H} and T Knudsen and Torben Johansen",
year = "1991",
language = "English",
volume = "279",
pages = "153--6",
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Differential effect of extracellular calcium on the Na(+)-K+ pump activity in intact polymorphonuclear leucocytes and erythrocytes. / Petersen, R H; Knudsen, T; Johansen, Torben.

I: F E B S Letters, Bind 279, Nr. 1, 1991, s. 153-6.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Differential effect of extracellular calcium on the Na(+)-K+ pump activity in intact polymorphonuclear leucocytes and erythrocytes

AU - Petersen, R H

AU - Knudsen, T

AU - Johansen, Torben

PY - 1991

Y1 - 1991

N2 - The effect of extracellular calcium on the Na(+)-K+ pump activity in human polymorphonuclear leucocytes and erythrocytes was studied and compared with the activity in mixed peritoneal leucocytes from rats. While there was maximal decrease in the pump activity (25-30%) of leucocytes from both rat and human by calcium 0.6 mM, a concentration of 0.1 mM caused a substantial decrease indicating a high sensitivity for extracellular calcium. In contrast, calcium had no effect on the pump activity in erythrocytes. The effect of calcium on the pump activity in leucocytes may be due to regulation of the influx of sodium across the plasma membrane, since in human leucocytes calcium had no effect on the pump activity if the cells were loaded with sodium.

AB - The effect of extracellular calcium on the Na(+)-K+ pump activity in human polymorphonuclear leucocytes and erythrocytes was studied and compared with the activity in mixed peritoneal leucocytes from rats. While there was maximal decrease in the pump activity (25-30%) of leucocytes from both rat and human by calcium 0.6 mM, a concentration of 0.1 mM caused a substantial decrease indicating a high sensitivity for extracellular calcium. In contrast, calcium had no effect on the pump activity in erythrocytes. The effect of calcium on the pump activity in leucocytes may be due to regulation of the influx of sodium across the plasma membrane, since in human leucocytes calcium had no effect on the pump activity if the cells were loaded with sodium.

KW - Animals

KW - Calcium

KW - Carrier Proteins

KW - Erythrocytes

KW - Humans

KW - Male

KW - Membrane Proteins

KW - Neutrophils

KW - Potassium

KW - Rats

KW - Rats, Inbred Strains

KW - Sodium

KW - Sodium-Potassium-Chloride Symporters

M3 - Journal article

C2 - 1995336

VL - 279

SP - 153

EP - 156

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 1

ER -