Dietary exposure to benzoxazinoids enhances bacteria-induced monokine responses by peripheral blood mononuclear cells

Dres Damgaard, Bettina Margrethe Jensen, Yaseelan Palarasah, Michael Friberg Bruun Nielsen, Khem Bahadur Adhikari, Heidi Julius Schnoor, Nanna Juel-Berg, Lars K Poulsen, Inge S Fomsgaard, Claus Henrik Nielsen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

SCOPE: To examine potentially immunomodulating effects of dietary benzoxazinoids (BXs), present in cereal grains.

METHODS AND RESULTS: Nineteen healthy volunteers were randomly distributed into two groups, who received diets with high or low content of BXs for three weeks. After a week's wash-out, the groups switched diets. Peripheral blood mononuclear cells (PBMCs) were stimulated with Porphyromonas gingivalis, Escherichia coli lipopolysaccharide (LPS), or tetanus toxoid (TT). PBMCs from a healthy donor received the same stimuli in presence of serum from each participant receiving BXs. The production of monokines, T-cell cytokines and T-helper cell proliferation were assessed. A three-week diet with high BX content enhanced IL-1β responses against LPS and P. gingivalis, as well as TNF-α response against P. gingivalis, after 24 hours of stimulation. Moreover, IL-6 was found to be increased after 7 days of stimulation with LPS. No effect was observed on T-cell cytokines or proliferation. BX levels in serum after a single meal did not modify cytokine responses.

CONCLUSIONS: High dietary intake of BXs enhances bacteria-induced production of pro-inflammatory monokines by PBMCs, but not T-cell responses; presumably due to intrinsic changes within PBMCs, built up over three weeks of BX-rich diet, rather than to immediate effects of BXs contained in serum. This article is protected by copyright. All rights reserved.

OriginalsprogEngelsk
TidsskriftMolecular Nutrition & Food Research
Vol/bind59
Udgave nummer11
Sider (fra-til)2190-2198
ISSN1613-4125
DOI
StatusUdgivet - 10. aug. 2015

Fingeraftryk

Benzoxazines
benzoxazinoids
dietary exposure
mononuclear leukocytes
bacteria
Porphyromonas gingivalis
T-lymphocytes
lipopolysaccharides
Diet
cytokines
diet
Serum
toxoids
tetanus
interleukin-1
interleukin-6
volunteers
Meals
food intake
small cereal grains

Citer dette

Damgaard, Dres ; Jensen, Bettina Margrethe ; Palarasah, Yaseelan ; Nielsen, Michael Friberg Bruun ; Adhikari, Khem Bahadur ; Schnoor, Heidi Julius ; Juel-Berg, Nanna ; Poulsen, Lars K ; Fomsgaard, Inge S ; Nielsen, Claus Henrik. / Dietary exposure to benzoxazinoids enhances bacteria-induced monokine responses by peripheral blood mononuclear cells. I: Molecular Nutrition & Food Research. 2015 ; Bind 59, Nr. 11. s. 2190-2198.
@article{2cc10bdc2cb249cdb47408d2f68ff2ed,
title = "Dietary exposure to benzoxazinoids enhances bacteria-induced monokine responses by peripheral blood mononuclear cells",
abstract = "SCOPE: To examine potentially immunomodulating effects of dietary benzoxazinoids (BXs), present in cereal grains.METHODS AND RESULTS: Nineteen healthy volunteers were randomly distributed into two groups, who received diets with high or low content of BXs for three weeks. After a week's wash-out, the groups switched diets. Peripheral blood mononuclear cells (PBMCs) were stimulated with Porphyromonas gingivalis, Escherichia coli lipopolysaccharide (LPS), or tetanus toxoid (TT). PBMCs from a healthy donor received the same stimuli in presence of serum from each participant receiving BXs. The production of monokines, T-cell cytokines and T-helper cell proliferation were assessed. A three-week diet with high BX content enhanced IL-1β responses against LPS and P. gingivalis, as well as TNF-α response against P. gingivalis, after 24 hours of stimulation. Moreover, IL-6 was found to be increased after 7 days of stimulation with LPS. No effect was observed on T-cell cytokines or proliferation. BX levels in serum after a single meal did not modify cytokine responses.CONCLUSIONS: High dietary intake of BXs enhances bacteria-induced production of pro-inflammatory monokines by PBMCs, but not T-cell responses; presumably due to intrinsic changes within PBMCs, built up over three weeks of BX-rich diet, rather than to immediate effects of BXs contained in serum. This article is protected by copyright. All rights reserved.",
author = "Dres Damgaard and Jensen, {Bettina Margrethe} and Yaseelan Palarasah and Nielsen, {Michael Friberg Bruun} and Adhikari, {Khem Bahadur} and Schnoor, {Heidi Julius} and Nanna Juel-Berg and Poulsen, {Lars K} and Fomsgaard, {Inge S} and Nielsen, {Claus Henrik}",
note = "This article is protected by copyright. All rights reserved.",
year = "2015",
month = "8",
day = "10",
doi = "10.1002/mnfr.201500151",
language = "English",
volume = "59",
pages = "2190--2198",
journal = "Molecular Nutrition & Food Research",
issn = "1613-4125",
publisher = "Wiley-VCH",
number = "11",

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Damgaard, D, Jensen, BM, Palarasah, Y, Nielsen, MFB, Adhikari, KB, Schnoor, HJ, Juel-Berg, N, Poulsen, LK, Fomsgaard, IS & Nielsen, CH 2015, 'Dietary exposure to benzoxazinoids enhances bacteria-induced monokine responses by peripheral blood mononuclear cells', Molecular Nutrition & Food Research, bind 59, nr. 11, s. 2190-2198. https://doi.org/10.1002/mnfr.201500151

Dietary exposure to benzoxazinoids enhances bacteria-induced monokine responses by peripheral blood mononuclear cells. / Damgaard, Dres; Jensen, Bettina Margrethe; Palarasah, Yaseelan; Nielsen, Michael Friberg Bruun; Adhikari, Khem Bahadur; Schnoor, Heidi Julius; Juel-Berg, Nanna; Poulsen, Lars K; Fomsgaard, Inge S; Nielsen, Claus Henrik.

I: Molecular Nutrition & Food Research, Bind 59, Nr. 11, 10.08.2015, s. 2190-2198.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Dietary exposure to benzoxazinoids enhances bacteria-induced monokine responses by peripheral blood mononuclear cells

AU - Damgaard, Dres

AU - Jensen, Bettina Margrethe

AU - Palarasah, Yaseelan

AU - Nielsen, Michael Friberg Bruun

AU - Adhikari, Khem Bahadur

AU - Schnoor, Heidi Julius

AU - Juel-Berg, Nanna

AU - Poulsen, Lars K

AU - Fomsgaard, Inge S

AU - Nielsen, Claus Henrik

N1 - This article is protected by copyright. All rights reserved.

PY - 2015/8/10

Y1 - 2015/8/10

N2 - SCOPE: To examine potentially immunomodulating effects of dietary benzoxazinoids (BXs), present in cereal grains.METHODS AND RESULTS: Nineteen healthy volunteers were randomly distributed into two groups, who received diets with high or low content of BXs for three weeks. After a week's wash-out, the groups switched diets. Peripheral blood mononuclear cells (PBMCs) were stimulated with Porphyromonas gingivalis, Escherichia coli lipopolysaccharide (LPS), or tetanus toxoid (TT). PBMCs from a healthy donor received the same stimuli in presence of serum from each participant receiving BXs. The production of monokines, T-cell cytokines and T-helper cell proliferation were assessed. A three-week diet with high BX content enhanced IL-1β responses against LPS and P. gingivalis, as well as TNF-α response against P. gingivalis, after 24 hours of stimulation. Moreover, IL-6 was found to be increased after 7 days of stimulation with LPS. No effect was observed on T-cell cytokines or proliferation. BX levels in serum after a single meal did not modify cytokine responses.CONCLUSIONS: High dietary intake of BXs enhances bacteria-induced production of pro-inflammatory monokines by PBMCs, but not T-cell responses; presumably due to intrinsic changes within PBMCs, built up over three weeks of BX-rich diet, rather than to immediate effects of BXs contained in serum. This article is protected by copyright. All rights reserved.

AB - SCOPE: To examine potentially immunomodulating effects of dietary benzoxazinoids (BXs), present in cereal grains.METHODS AND RESULTS: Nineteen healthy volunteers were randomly distributed into two groups, who received diets with high or low content of BXs for three weeks. After a week's wash-out, the groups switched diets. Peripheral blood mononuclear cells (PBMCs) were stimulated with Porphyromonas gingivalis, Escherichia coli lipopolysaccharide (LPS), or tetanus toxoid (TT). PBMCs from a healthy donor received the same stimuli in presence of serum from each participant receiving BXs. The production of monokines, T-cell cytokines and T-helper cell proliferation were assessed. A three-week diet with high BX content enhanced IL-1β responses against LPS and P. gingivalis, as well as TNF-α response against P. gingivalis, after 24 hours of stimulation. Moreover, IL-6 was found to be increased after 7 days of stimulation with LPS. No effect was observed on T-cell cytokines or proliferation. BX levels in serum after a single meal did not modify cytokine responses.CONCLUSIONS: High dietary intake of BXs enhances bacteria-induced production of pro-inflammatory monokines by PBMCs, but not T-cell responses; presumably due to intrinsic changes within PBMCs, built up over three weeks of BX-rich diet, rather than to immediate effects of BXs contained in serum. This article is protected by copyright. All rights reserved.

U2 - 10.1002/mnfr.201500151

DO - 10.1002/mnfr.201500151

M3 - Journal article

VL - 59

SP - 2190

EP - 2198

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

SN - 1613-4125

IS - 11

ER -