Diamond like carbon Ag nanocomposites as a control measure against Campylobacter jejuni and Listeria monocytogenes on food preparation surfaces

Gintarė Zakarienė*, Aleksandr Novoslavskij, Šarūnas Meškinis, Andrius Vasiliauskas, Asta Tamulevičienė, Sigitas Tamulevičius, Thomas Alter, Mindaugas Malakauskas

*Kontaktforfatter for dette arbejde

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Abstrakt

The effect of thin (5 nm) and thick (40 nm) silver layers and diamond like carbon nanocomposites with embedded Ag nanoparticles (DLC:Ag) against two reference strains of C. jejuni NCTC 11168 and L. monocytogenes ATCC 7644 were evaluated in this study. DLC:Ag film contained 22 at.% Ag. Silver nanoparticle size measured by transmission electron microscope was in the 5–10 nm range. Ag layers and DLC:Ag nanocomposites were deposited employing unbalanced reactive magnetron sputtering on crystalline silicon wafers. C. jejuni and L. monocytogenes numbers were counted by culture-based enumeration on selective agars and quantitative real-time PCR (qPCR) including staining with propidium monoazide (PMA). It was determined, that DLC:Ag film was the most efficient coating in the reduction of C. jejuni and L. monocytogenes numbers. Culture-based enumeration revealed that C. jejuni numbers were reduced by an average of 4.06 log10CFU/ml after 15 min and 3.61 log10CFU/ml after 30 min on DLC:Ag coated silicon wafers in comparison to control samples (P ≤ 0.05). L. monocytogenes was not detected on DLC:Ag samples after 24 h of exposure (P ≤ 0.05). PMA-qPCR showed that C. jejuni and L. monocytogenes affected by DLC:Ag antimicrobial surface showed a reduced ability to grow on culture media, but maintained viability during the whole experiment. Nonetheless, DLC:Ag antimicrobial surface could be further considered for the reduction of cross-contamination risk from food preparation surfaces due to their contamination with C. jejuni and L. monocytogenes in domestic and commercial kitchens or food establishments.

OriginalsprogEngelsk
TidsskriftDiamond and Related Materials
Vol/bind81
Sider (fra-til)118-126
ISSN0925-9635
DOI
StatusUdgivet - 2018
Udgivet eksterntJa

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