Development of a compact Bio-Optofluidic Cell Sorter

Andrew Rafael Bañas, Darwin Palima, Finn Pedersen, Jesper Glückstad

Publikation: Kapitel i bog/rapport/konference-proceedingKonferencebidrag i proceedingsForskningpeer review

Abstrakt

We develop an active cell sorter that utilizes machine vision for cell identification. Particles are identified based on visual features such as shape, size and color using image processing. The sorter shares features from our previously developed BioPhotonics Workstation. Hence, it benefits from the extended axial manipulation range provided by the low numerical aperture geometry. Detected particles are catapulted axially by several hundred microns, allowing them to be moved from one laminar flow region to another. As the sorting motion is transverse to the viewing plane, multiple particles can be catapulted at the same time, therefore enabling parallel sorting. The sorter is developed with a minimal footprint such that it can operate as a table top device, an advantage over flow cytometry or FACS systems.
OriginalsprogEngelsk
TitelProceedings of SPIE : Complex Light and Optical Forces VI
ForlagSPIE - International Society for Optical Engineering
Publikationsdato10. feb. 2012
Artikelnummer82740N
DOI
StatusUdgivet - 10. feb. 2012
Udgivet eksterntJa
BegivenhedSPIE OPTO: Complex Light and Optical Forces VI - San Francisco, USA
Varighed: 21. jan. 201226. jan. 2012

Konference

KonferenceSPIE OPTO
LandUSA
BySan Francisco
Periode21/01/201226/01/2012
NavnProceedings of SPIE, the International Society for Optical Engineering
Vol/bind8274
ISSN0277-786X

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