Determining factors for the nano-biocompatibility of cobalt oxide nanoparticles: proximal discrepancy in intrinsic atomic interactions at differential vicinage

Suresh K. Verma*, Pritam Kumar Panda, Puja Kumari, Paritosh Patel, Aryashree Arunima, Eealisha Jha, Shaheen Husain, Ravi Prakash, Roland Hergenröder, Yogendra Kumar Mishra, Rajeev Ahuja, Rajender S. Varma*, Mrutyunjay Suar*

*Kontaktforfatter for dette arbejde

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Abstrakt

The abounding use of cobalt oxide nanoparticles (Co 3O 4) requires a detailed understanding of their environmental and biomedical nanotoxicity and an eminent solution to the associated hazards; molecular and atomic aspects of the subject are poorly understood. This study reconnoiters thein vitroandin vivonanotoxicity of Co 3O 4nanoparticles using human colon cell lines and the embryonic zebrafish model. The synthesis of Co 3O 4nanoparticles (G-CoONP) is delineatedviathe deployment of a medicinal herb,Calotropis gigantea, as an alternative greener solution; stable G-CoONP with a size of 41 ± 15 nm are attainable. Gas chromatography-mass spectroscopy (GCMS) analysis revealed the role of floral extract biomolecules in G-CoO NP synthesis. Thein vitroandin vivoeffects are accompanied by dose-dependent exposure at the molecular level by eliciting Sod1 and P53 genes up to 8.2 and 5.2 fold leading to a significant change in the reactive oxygen species and apoptosis level. It unraveled the toxicity of the cobalt oxide NP as increased apoptosis elicited by higher oxidative stress due to the accumulation and internalization of nanoparticles in cells and embryos. Green synthesized G-CoONP exhibited higher biocompatibility than commercial C-CoONP with reduced apoptosis and ROS in both human colon cell lines and zebrafish embryos.In silicoanalysis portrayed the intrinsic atomic interaction of Co 3O 4NP with cysteine, arginine, and histidine of oxidative stress (SOD1/sod1) and apoptosis (TP53/tp53) proteins leading to dysregulation of their structural and functional integrity in human and zebrafish, respectively. A proximal discrepancy in intrinsic atomic interaction due to the H-bonding and hydrophobic interaction at the differentialin vitroandin vivovicinage served as a key determinant factor for the cellular biocompatibility of Co 3O 4nanoparticles.

OriginalsprogEngelsk
TidsskriftGreen Chemistry
Vol/bind23
Udgave nummer9
Sider (fra-til)3439-3458
ISSN1463-9262
DOI
StatusUdgivet - 7. maj 2021

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