Determination of autoantibodies by line immunoblotting

Louise Sternbæk*, Tina Friis, Gunnar Houen

*Kontaktforfatter for dette arbejde

Publikation: Kapitel i bog/rapport/konference-proceedingKapitel i bogForskningpeer review

Abstrakt

Autoantibody detection is a useful and obligatory tool for clinicians and researchers in diagnosing autoimmune diseases. Line blotting is a simple, sensitive, and flexible technique for fast semiquantitative detection of multiple antibodies. Line blotting enables the detection of antibodies on membrane strips coated with thin parallel lines of several purified, biochemically characterized antigens, which are fixed onto a synthetic support. Each strip can contain more than ten antigens, thus allowing simultaneous screening for multiple antibodies. Here, we describe the principle of line blotting and how the buffer composition can interfere with the results of autoantibody detection.

OriginalsprogEngelsk
TitelAutoantibodies : Methods and Protocols
RedaktørerGunnar Houen
Vol/bind1901
ForlagHumana Press
Publikationsdato1. jan. 2019
Sider255-262
ISBN (Trykt)978-1-4939-8948-5
ISBN (Elektronisk)978-1-4939-8949-2
DOI
StatusUdgivet - 1. jan. 2019
NavnMethods in Molecular Biology
Vol/bind1901
ISSN1064-3745

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