Detection of proteolytic signatures for Parkinson's disease

Peter Lüttge Jordal, Thomas Franck Dyrlund, Kristian Winge, Martin R. Larsen, Erik H Danielsen, James A. Wells, Daniel E. Otzen, Jan J. Enghild*

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Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Aim: To investigate if idiopathic Parkinson's disease (IPD) is associated with distinct proteolytic signatures relative to non-neurodegenerative controls (NND) and patients with multiple system atrophy (MSA). Materials & methods: A subtiligase-based N-terminomics screening method was exploited for semiquantitative comparison of protein N-termini in cerebrospinal fluid for pooled samples of IPD (n = 6) and NND (n = 8) individuals. Subsequently, targeted selected reaction monitoring mass spectrometry measured the relative concentration of the proteolytic signature peptides in individual IPD (n = 22), NND (n = 11) and MSA (n = 18) samples. Results: The discovery screen detected 300 N-termini for 156 proteins. Selected reaction monitoring analysis revealed that two of these peptides differentiate IPD from NND while three peptides differentiate IPD from MSA. Conclusion: IPD is associated with distinct proteolytic signatures.

OriginalsprogEngelsk
TidsskriftFuture Neurology
Vol/bind11
Udgave nummer1
Sider (fra-til)15-32
ISSN1479-6708
DOI
StatusUdgivet - 1. feb. 2016

Fingeraftryk

Parkinson Disease
Peptides
Cerebrospinal Fluid
Proteins

Citer dette

Jordal, P. L., Dyrlund, T. F., Winge, K., Larsen, M. R., Danielsen, E. H., Wells, J. A., ... Enghild, J. J. (2016). Detection of proteolytic signatures for Parkinson's disease. Future Neurology, 11(1), 15-32. https://doi.org/10.2217/fnl.16.3
Jordal, Peter Lüttge ; Dyrlund, Thomas Franck ; Winge, Kristian ; Larsen, Martin R. ; Danielsen, Erik H ; Wells, James A. ; Otzen, Daniel E. ; Enghild, Jan J. / Detection of proteolytic signatures for Parkinson's disease. I: Future Neurology. 2016 ; Bind 11, Nr. 1. s. 15-32.
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abstract = "Aim: To investigate if idiopathic Parkinson's disease (IPD) is associated with distinct proteolytic signatures relative to non-neurodegenerative controls (NND) and patients with multiple system atrophy (MSA). Materials & methods: A subtiligase-based N-terminomics screening method was exploited for semiquantitative comparison of protein N-termini in cerebrospinal fluid for pooled samples of IPD (n = 6) and NND (n = 8) individuals. Subsequently, targeted selected reaction monitoring mass spectrometry measured the relative concentration of the proteolytic signature peptides in individual IPD (n = 22), NND (n = 11) and MSA (n = 18) samples. Results: The discovery screen detected 300 N-termini for 156 proteins. Selected reaction monitoring analysis revealed that two of these peptides differentiate IPD from NND while three peptides differentiate IPD from MSA. Conclusion: IPD is associated with distinct proteolytic signatures.",
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Jordal, PL, Dyrlund, TF, Winge, K, Larsen, MR, Danielsen, EH, Wells, JA, Otzen, DE & Enghild, JJ 2016, 'Detection of proteolytic signatures for Parkinson's disease', Future Neurology, bind 11, nr. 1, s. 15-32. https://doi.org/10.2217/fnl.16.3

Detection of proteolytic signatures for Parkinson's disease. / Jordal, Peter Lüttge; Dyrlund, Thomas Franck; Winge, Kristian; Larsen, Martin R.; Danielsen, Erik H; Wells, James A.; Otzen, Daniel E.; Enghild, Jan J.

I: Future Neurology, Bind 11, Nr. 1, 01.02.2016, s. 15-32.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Detection of proteolytic signatures for Parkinson's disease

AU - Jordal, Peter Lüttge

AU - Dyrlund, Thomas Franck

AU - Winge, Kristian

AU - Larsen, Martin R.

AU - Danielsen, Erik H

AU - Wells, James A.

AU - Otzen, Daniel E.

AU - Enghild, Jan J.

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Aim: To investigate if idiopathic Parkinson's disease (IPD) is associated with distinct proteolytic signatures relative to non-neurodegenerative controls (NND) and patients with multiple system atrophy (MSA). Materials & methods: A subtiligase-based N-terminomics screening method was exploited for semiquantitative comparison of protein N-termini in cerebrospinal fluid for pooled samples of IPD (n = 6) and NND (n = 8) individuals. Subsequently, targeted selected reaction monitoring mass spectrometry measured the relative concentration of the proteolytic signature peptides in individual IPD (n = 22), NND (n = 11) and MSA (n = 18) samples. Results: The discovery screen detected 300 N-termini for 156 proteins. Selected reaction monitoring analysis revealed that two of these peptides differentiate IPD from NND while three peptides differentiate IPD from MSA. Conclusion: IPD is associated with distinct proteolytic signatures.

AB - Aim: To investigate if idiopathic Parkinson's disease (IPD) is associated with distinct proteolytic signatures relative to non-neurodegenerative controls (NND) and patients with multiple system atrophy (MSA). Materials & methods: A subtiligase-based N-terminomics screening method was exploited for semiquantitative comparison of protein N-termini in cerebrospinal fluid for pooled samples of IPD (n = 6) and NND (n = 8) individuals. Subsequently, targeted selected reaction monitoring mass spectrometry measured the relative concentration of the proteolytic signature peptides in individual IPD (n = 22), NND (n = 11) and MSA (n = 18) samples. Results: The discovery screen detected 300 N-termini for 156 proteins. Selected reaction monitoring analysis revealed that two of these peptides differentiate IPD from NND while three peptides differentiate IPD from MSA. Conclusion: IPD is associated with distinct proteolytic signatures.

KW - cerebrospinal fluid

KW - N-terminomics

KW - Parkinson's disease

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DO - 10.2217/fnl.16.3

M3 - Journal article

AN - SCOPUS:84975757822

VL - 11

SP - 15

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JO - Future Neurology

JF - Future Neurology

SN - 1479-6708

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Jordal PL, Dyrlund TF, Winge K, Larsen MR, Danielsen EH, Wells JA et al. Detection of proteolytic signatures for Parkinson's disease. Future Neurology. 2016 feb 1;11(1):15-32. https://doi.org/10.2217/fnl.16.3