Detection of membrane-bound and soluble antigens by magnetic levitation

Mikkel Schou Andersen, Emily Howard, Shulin Lu, Matthew Richard, Mark Gregory, Gordon Ogembo, Ofer Mazor, Pavel Gorelik, Nathan I. Shapiro, Anish V. Sharda, Ionita Ghiran*

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstrakt

Magnetic levitation is a technique for measuring the density and the magnetic properties of objects suspended in a paramagnetic field. We describe a novel magnetic levitation-based method that can specifically detect cell membrane-bound and soluble antigens by measurable changes in levitation height that result from the formation of antibody-coated bead and antigen complex. We demonstrate our method's ability to sensitively detect an array of membrane-bound and soluble antigens found in blood, including T-cell antigen CD3, eosinophil antigen Siglec-8, red blood cell antigens CD35 and RhD, red blood cell-bound Epstein-Barr viral particles, and soluble IL-6, and validate the results by flow cytometry and immunofluorescence microscopy performed in parallel. Additionally, employing an inexpensive, single lens, manual focus, wifi-enabled camera, we extend the portability of our method for its potential use as a point-of-care diagnostic assay.

OriginalsprogEngelsk
TidsskriftLab on a Chip
Vol/bind17
Udgave nummer20
Sider (fra-til)3462-3473
Antal sider12
ISSN1473-0197
DOI
StatusUdgivet - 2017

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