Detection of major histocompatibility complex molecules in processed allogeneic bone blocks for use in alveolar ridge reconstruction

Tobias Fretwurst, Lames M Gad, Thorsten Steinberg, Hagen Schmal, Robert Zeiser, Anna-K Amler, Katja Nelson, Brigitte Altmann

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OBJECTIVES: Because processed allogenic bone blocks contain remnants of cells and other organic material, the present study examined the putative presence of major histocompatibility complex (MHC) molecules in protein extracts derived from processed allogeneic bone blocks.

STUDY DESIGN: Protein content and the immunogenic potential of 3 different processed allografts (Osteograft, DIZG, Berlin, Germany; Caput femoris, DIZG, Berlin, Germany; Human Spongiosa, Charité Tissue Bank, Berlin, Germany) were assessed by protein extraction and analysis of the presence of MHC class 1 and 2 molecules prior to grafting. MHC concentration was measured by using enzyme-linked immunosorbent assay.

RESULTS: Protein content in the allograft materials varied between 0.87 and 1.61 µg protein/mg. In the allograft Human Spongiosa, no MHC was detected, whereas in the allogeneic bone blocks Osteograft and Caput femoris MHC 1 (0.04-0.037 ng/mg graft material) and in Osteograft MHC class 2 molecules were detectable.

CONCLUSIONS: The results of the present study suggest that despite thorough processing, a potential antigenicity of allografts is not eliminated. MHC molecules in allografts may sensitize the immune system.

TidsskriftOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
Udgave nummer1
Sider (fra-til)16-21
StatusUdgivet - 20. mar. 2018