Detection of autoimmune antibodies in localized scleroderma by synthetic oligonucleotide antigens

Simone Samuelsen, Christian Damsgaard Jørgensen, Elizabeth D Mellins, Kathryn S Torok, Kira Astakhova

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Resumé

In this study, we developed a series of synthetic oligonucleotides that allowed us to investigate the details on the antigen recognition by autoimmune antibodies in localized scleroderma subjects. Besides dramatically improved analytical specificity of the assay, our data suggests a potential linking for antibodies to DNA to the biological status of disease state in localized scleroderma. Moreover, introducing chemical modifications into short synthetic deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) molecules completely changed the binding titers of corresponding antibodies and their clinical relevance. The strongest observed effect was registered for the localized scleroderma skin damage index (LoSDI) on the IgG antibodies to TC dinucleotide-rich double-stranded antigen (p < 0.001). In addition to providing valuable tools for diagnosis of clinically relevant biomarkers, we believe that this work opens up new opportunities for research on antibodies to nucleic acids in localized scleroderma and other autoimmune diseases.

OriginalsprogEngelsk
Artikelnummere0195381
TidsskriftPLOS ONE
Vol/bind13
Udgave nummer4
Antal sider12
ISSN1932-6203
DOI
StatusUdgivet - 2018

Fingeraftryk

Localized Scleroderma
oligonucleotides
Oligonucleotides
antigens
Antigens
antibodies
Antibodies
analytical specificity
autoimmune diseases
DNA
Chemical modification
Biomarkers
Nucleic Acids
nucleic acids
Assays
biomarkers
Skin
Immunoglobulin G
RNA
Molecules

Citer dette

Samuelsen, Simone ; Jørgensen, Christian Damsgaard ; Mellins, Elizabeth D ; Torok, Kathryn S ; Astakhova, Kira. / Detection of autoimmune antibodies in localized scleroderma by synthetic oligonucleotide antigens. I: PLOS ONE. 2018 ; Bind 13, Nr. 4.
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title = "Detection of autoimmune antibodies in localized scleroderma by synthetic oligonucleotide antigens",
abstract = "In this study, we developed a series of synthetic oligonucleotides that allowed us to investigate the details on the antigen recognition by autoimmune antibodies in localized scleroderma subjects. Besides dramatically improved analytical specificity of the assay, our data suggests a potential linking for antibodies to DNA to the biological status of disease state in localized scleroderma. Moreover, introducing chemical modifications into short synthetic deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) molecules completely changed the binding titers of corresponding antibodies and their clinical relevance. The strongest observed effect was registered for the localized scleroderma skin damage index (LoSDI) on the IgG antibodies to TC dinucleotide-rich double-stranded antigen (p < 0.001). In addition to providing valuable tools for diagnosis of clinically relevant biomarkers, we believe that this work opens up new opportunities for research on antibodies to nucleic acids in localized scleroderma and other autoimmune diseases.",
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Detection of autoimmune antibodies in localized scleroderma by synthetic oligonucleotide antigens. / Samuelsen, Simone; Jørgensen, Christian Damsgaard; Mellins, Elizabeth D; Torok, Kathryn S; Astakhova, Kira.

I: PLOS ONE, Bind 13, Nr. 4, e0195381, 2018.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Detection of autoimmune antibodies in localized scleroderma by synthetic oligonucleotide antigens

AU - Samuelsen, Simone

AU - Jørgensen, Christian Damsgaard

AU - Mellins, Elizabeth D

AU - Torok, Kathryn S

AU - Astakhova, Kira

PY - 2018

Y1 - 2018

N2 - In this study, we developed a series of synthetic oligonucleotides that allowed us to investigate the details on the antigen recognition by autoimmune antibodies in localized scleroderma subjects. Besides dramatically improved analytical specificity of the assay, our data suggests a potential linking for antibodies to DNA to the biological status of disease state in localized scleroderma. Moreover, introducing chemical modifications into short synthetic deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) molecules completely changed the binding titers of corresponding antibodies and their clinical relevance. The strongest observed effect was registered for the localized scleroderma skin damage index (LoSDI) on the IgG antibodies to TC dinucleotide-rich double-stranded antigen (p < 0.001). In addition to providing valuable tools for diagnosis of clinically relevant biomarkers, we believe that this work opens up new opportunities for research on antibodies to nucleic acids in localized scleroderma and other autoimmune diseases.

AB - In this study, we developed a series of synthetic oligonucleotides that allowed us to investigate the details on the antigen recognition by autoimmune antibodies in localized scleroderma subjects. Besides dramatically improved analytical specificity of the assay, our data suggests a potential linking for antibodies to DNA to the biological status of disease state in localized scleroderma. Moreover, introducing chemical modifications into short synthetic deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) molecules completely changed the binding titers of corresponding antibodies and their clinical relevance. The strongest observed effect was registered for the localized scleroderma skin damage index (LoSDI) on the IgG antibodies to TC dinucleotide-rich double-stranded antigen (p < 0.001). In addition to providing valuable tools for diagnosis of clinically relevant biomarkers, we believe that this work opens up new opportunities for research on antibodies to nucleic acids in localized scleroderma and other autoimmune diseases.

KW - Antigens/chemistry

KW - Autoantibodies/analysis

KW - Humans

KW - Oligonucleotides/chemistry

KW - Scleroderma, Localized/immunology

U2 - 10.1371/journal.pone.0195381

DO - 10.1371/journal.pone.0195381

M3 - Journal article

C2 - 29641558

VL - 13

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 4

M1 - e0195381

ER -