Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome

Morad Kamand; Mirolyuba Ilieva; Sheena Louise Forsberg; Mads Thomassen; Morten Meyer; Åsa Fex Svenningsen; Tanja Maria Michel

doi:10.1016/j.scr.2020.101974

Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome

Morad Kamand^*, Mirolyuba Ilieva, Sheena Louise Forsberg, Mads Thomassen, Morten Meyer, Åsa Fex Svenningsen, Tanja Maria Michel^*

^*Kontaktforfatter

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

104 Downloads (Pure)

Abstract

Autism spectrum disorder is a heterogenous neurodevelopmental disorder. The patients experience challenges in social interaction and communication skills as well as restricted and/or repetitive behaviors. To understand the molecular mechanisms underlying developmental brain disorders, patient-derived cellular models represent a useful tool. We have generated a human induced pluripotent stem cell line (SDUKIi003-A) from skin fibroblasts derived from a 20-year old male patient diagnosed with Asperger syndrome (“FYNEN-cohort” of Southern Denmark). The reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

Originalsprog	Engelsk
Artikelnummer	101974
Tidsskrift	Stem Cell Research
Vol/bind	48
Antal sider	5
ISSN	1873-5061
DOI	https://doi.org/10.1016/j.scr.2020.101974
Status	Udgivet - okt. 2020

Dokumenter og links

10.1016/j.scr.2020.101974Licens: CC BY-NC-ND

Open Access VersionForlagets udgivne version, 2,04 MBLicens: CC BY-NC-ND

1 Ph.d.-afhandling

Studying autism spectrum disorders (ASD) using a three-dimensional brain organoid model derived from patient-specific induced pluripotent stem cells (iPSCs): Autism from skin to brain
Kamand, M., 24. jun. 2022, Syddansk Universitet. Det Sundhedsvidenskabelige Fakultet. 225 s.
Publikation: Afhandling › Ph.d.-afhandling

Citationsformater

@article{4b543326b6af4e189df0cc507126508f,

title = "Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome",

abstract = "Autism spectrum disorder is a heterogenous neurodevelopmental disorder. The patients experience challenges in social interaction and communication skills as well as restricted and/or repetitive behaviors. To understand the molecular mechanisms underlying developmental brain disorders, patient-derived cellular models represent a useful tool. We have generated a human induced pluripotent stem cell line (SDUKIi003-A) from skin fibroblasts derived from a 20-year old male patient diagnosed with Asperger syndrome (“FYNEN-cohort” of Southern Denmark). The reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.",

author = "Morad Kamand and Mirolyuba Ilieva and {Louise Forsberg}, Sheena and Mads Thomassen and Morten Meyer and {Fex Svenningsen}, {\AA}sa and {Maria Michel}, Tanja",

year = "2020",

month = oct,

doi = "10.1016/j.scr.2020.101974",

language = "English",

volume = "48",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

}

TY - JOUR

T1 - Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome

AU - Kamand, Morad

AU - Ilieva, Mirolyuba

AU - Louise Forsberg, Sheena

AU - Thomassen, Mads

AU - Meyer, Morten

AU - Fex Svenningsen, Åsa

AU - Maria Michel, Tanja

PY - 2020/10

Y1 - 2020/10

N2 - Autism spectrum disorder is a heterogenous neurodevelopmental disorder. The patients experience challenges in social interaction and communication skills as well as restricted and/or repetitive behaviors. To understand the molecular mechanisms underlying developmental brain disorders, patient-derived cellular models represent a useful tool. We have generated a human induced pluripotent stem cell line (SDUKIi003-A) from skin fibroblasts derived from a 20-year old male patient diagnosed with Asperger syndrome (“FYNEN-cohort” of Southern Denmark). The reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

AB - Autism spectrum disorder is a heterogenous neurodevelopmental disorder. The patients experience challenges in social interaction and communication skills as well as restricted and/or repetitive behaviors. To understand the molecular mechanisms underlying developmental brain disorders, patient-derived cellular models represent a useful tool. We have generated a human induced pluripotent stem cell line (SDUKIi003-A) from skin fibroblasts derived from a 20-year old male patient diagnosed with Asperger syndrome (“FYNEN-cohort” of Southern Denmark). The reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

U2 - 10.1016/j.scr.2020.101974

DO - 10.1016/j.scr.2020.101974

M3 - Journal article

C2 - 32916638

AN - SCOPUS:85090357129

SN - 1873-5061

VL - 48

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 101974

ER -

Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome

Abstract

Dokumenter og links

Fingeraftryk

Relaterede publikationer

Studying autism spectrum disorders (ASD) using a three-dimensional brain organoid model derived from patient-specific induced pluripotent stem cells (iPSCs): Autism from skin to brain

Citationsformater