Derivation from an alloreactive T-cell line of a clone which cross-reacts with a self H2-E-restricted minor alloantigen

T Owens, M E Liddell, I N Crispe

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 1984-May
OriginalsprogEngelsk
TidsskriftCellular Immunology
Vol/bind85
Udgave nummer2
Sider (fra-til)436-46
Antal sider10
ISSN0008-8749
StatusUdgivet - 1984

Fingeraftryk

Blocking Antibodies
Clone Cells
Cross Reactions
Cell Line
Organism Cloning

Citer dette

@article{19303770cf0511deb0fe000ea68e967b,
title = "Derivation from an alloreactive T-cell line of a clone which cross-reacts with a self H2-E-restricted minor alloantigen",
abstract = "An alloreactive T-helper-cell line [(A.TH X Balb/c) anti-A.TL] was shown to recognize both H2-Ek and H2-Ed. Both proliferation and polyclonal B-cell activation (protein A plaques) were used in the analyses of specificity. On cloning, the H2-Ek/Ed cross-reaction was shown by one clonotype. This cross-reaction is interpreted in light of the P. Matzinger and M.J. Bevan model (Cell. Immunol. 29, 1, 1977) for antigen recognition by T cells. The antigen recognized was a cell surface non-major histocompatibility (MHC) (minor) alloantigen, shared by the DBA and B10 backgrounds, whose recognition was H2-Ed restricted. This clone is therefore both alloreactive and self-MHC (Ed) restricted minor reactive. Other clones from the cell line were either allospecific or autoreactive. It was shown by antibody blocking that the H2-E alpha (Ia7) determinants involved in the cross-reactive and allospecific recognition were not the same. By antibody blocking it was also shown that the antigenic determinant recognized by the autoreactive clone included H2-Ed. The alloreactive cell line therefore contained three H2-E-reactive clonotypes: allo-Ek specific, H2-Ek and DBA/B10 minor plus H2-Ed cross-reactive, and Ed-associated autoreactive.",
keywords = "Animals, Antibodies, Monoclonal, Antigens, Surface, Autoantigens, Binding, Competitive, Cell Line, Clone Cells, Cross Reactions, H-2 Antigens, Haploidy, Histocompatibility Antigens Class II, Lymphocyte Activation, Mice, Mice, Inbred A, Mice, Inbred AKR, Mice, Inbred BALB C, Mice, Inbred C3H, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Inbred DBA, Minor Histocompatibility Loci, Phenotype, T-Lymphocytes",
author = "T Owens and Liddell, {M E} and Crispe, {I N}",
year = "1984",
language = "English",
volume = "85",
pages = "436--46",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Heinemann",
number = "2",

}

Derivation from an alloreactive T-cell line of a clone which cross-reacts with a self H2-E-restricted minor alloantigen. / Owens, T; Liddell, M E; Crispe, I N.

I: Cellular Immunology, Bind 85, Nr. 2, 1984, s. 436-46.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Derivation from an alloreactive T-cell line of a clone which cross-reacts with a self H2-E-restricted minor alloantigen

AU - Owens, T

AU - Liddell, M E

AU - Crispe, I N

PY - 1984

Y1 - 1984

N2 - An alloreactive T-helper-cell line [(A.TH X Balb/c) anti-A.TL] was shown to recognize both H2-Ek and H2-Ed. Both proliferation and polyclonal B-cell activation (protein A plaques) were used in the analyses of specificity. On cloning, the H2-Ek/Ed cross-reaction was shown by one clonotype. This cross-reaction is interpreted in light of the P. Matzinger and M.J. Bevan model (Cell. Immunol. 29, 1, 1977) for antigen recognition by T cells. The antigen recognized was a cell surface non-major histocompatibility (MHC) (minor) alloantigen, shared by the DBA and B10 backgrounds, whose recognition was H2-Ed restricted. This clone is therefore both alloreactive and self-MHC (Ed) restricted minor reactive. Other clones from the cell line were either allospecific or autoreactive. It was shown by antibody blocking that the H2-E alpha (Ia7) determinants involved in the cross-reactive and allospecific recognition were not the same. By antibody blocking it was also shown that the antigenic determinant recognized by the autoreactive clone included H2-Ed. The alloreactive cell line therefore contained three H2-E-reactive clonotypes: allo-Ek specific, H2-Ek and DBA/B10 minor plus H2-Ed cross-reactive, and Ed-associated autoreactive.

AB - An alloreactive T-helper-cell line [(A.TH X Balb/c) anti-A.TL] was shown to recognize both H2-Ek and H2-Ed. Both proliferation and polyclonal B-cell activation (protein A plaques) were used in the analyses of specificity. On cloning, the H2-Ek/Ed cross-reaction was shown by one clonotype. This cross-reaction is interpreted in light of the P. Matzinger and M.J. Bevan model (Cell. Immunol. 29, 1, 1977) for antigen recognition by T cells. The antigen recognized was a cell surface non-major histocompatibility (MHC) (minor) alloantigen, shared by the DBA and B10 backgrounds, whose recognition was H2-Ed restricted. This clone is therefore both alloreactive and self-MHC (Ed) restricted minor reactive. Other clones from the cell line were either allospecific or autoreactive. It was shown by antibody blocking that the H2-E alpha (Ia7) determinants involved in the cross-reactive and allospecific recognition were not the same. By antibody blocking it was also shown that the antigenic determinant recognized by the autoreactive clone included H2-Ed. The alloreactive cell line therefore contained three H2-E-reactive clonotypes: allo-Ek specific, H2-Ek and DBA/B10 minor plus H2-Ed cross-reactive, and Ed-associated autoreactive.

KW - Animals

KW - Antibodies, Monoclonal

KW - Antigens, Surface

KW - Autoantigens

KW - Binding, Competitive

KW - Cell Line

KW - Clone Cells

KW - Cross Reactions

KW - H-2 Antigens

KW - Haploidy

KW - Histocompatibility Antigens Class II

KW - Lymphocyte Activation

KW - Mice

KW - Mice, Inbred A

KW - Mice, Inbred AKR

KW - Mice, Inbred BALB C

KW - Mice, Inbred C3H

KW - Mice, Inbred C57BL

KW - Mice, Inbred CBA

KW - Mice, Inbred DBA

KW - Minor Histocompatibility Loci

KW - Phenotype

KW - T-Lymphocytes

M3 - Journal article

C2 - 6609002

VL - 85

SP - 436

EP - 446

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 2

ER -