Resumé
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Cellular Immunology |
| Vol/bind | 85 |
| Udgave nummer | 2 |
| Sider (fra-til) | 436-46 |
| Antal sider | 10 |
| ISSN | 0008-8749 |
| Status | Udgivet - 1984 |
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Derivation from an alloreactive T-cell line of a clone which cross-reacts with a self H2-E-restricted minor alloantigen. / Owens, T; Liddell, M E; Crispe, I N.
I: Cellular Immunology, Bind 85, Nr. 2, 1984, s. 436-46.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
TY - JOUR
T1 - Derivation from an alloreactive T-cell line of a clone which cross-reacts with a self H2-E-restricted minor alloantigen
AU - Owens, T
AU - Liddell, M E
AU - Crispe, I N
PY - 1984
Y1 - 1984
N2 - An alloreactive T-helper-cell line [(A.TH X Balb/c) anti-A.TL] was shown to recognize both H2-Ek and H2-Ed. Both proliferation and polyclonal B-cell activation (protein A plaques) were used in the analyses of specificity. On cloning, the H2-Ek/Ed cross-reaction was shown by one clonotype. This cross-reaction is interpreted in light of the P. Matzinger and M.J. Bevan model (Cell. Immunol. 29, 1, 1977) for antigen recognition by T cells. The antigen recognized was a cell surface non-major histocompatibility (MHC) (minor) alloantigen, shared by the DBA and B10 backgrounds, whose recognition was H2-Ed restricted. This clone is therefore both alloreactive and self-MHC (Ed) restricted minor reactive. Other clones from the cell line were either allospecific or autoreactive. It was shown by antibody blocking that the H2-E alpha (Ia7) determinants involved in the cross-reactive and allospecific recognition were not the same. By antibody blocking it was also shown that the antigenic determinant recognized by the autoreactive clone included H2-Ed. The alloreactive cell line therefore contained three H2-E-reactive clonotypes: allo-Ek specific, H2-Ek and DBA/B10 minor plus H2-Ed cross-reactive, and Ed-associated autoreactive.
AB - An alloreactive T-helper-cell line [(A.TH X Balb/c) anti-A.TL] was shown to recognize both H2-Ek and H2-Ed. Both proliferation and polyclonal B-cell activation (protein A plaques) were used in the analyses of specificity. On cloning, the H2-Ek/Ed cross-reaction was shown by one clonotype. This cross-reaction is interpreted in light of the P. Matzinger and M.J. Bevan model (Cell. Immunol. 29, 1, 1977) for antigen recognition by T cells. The antigen recognized was a cell surface non-major histocompatibility (MHC) (minor) alloantigen, shared by the DBA and B10 backgrounds, whose recognition was H2-Ed restricted. This clone is therefore both alloreactive and self-MHC (Ed) restricted minor reactive. Other clones from the cell line were either allospecific or autoreactive. It was shown by antibody blocking that the H2-E alpha (Ia7) determinants involved in the cross-reactive and allospecific recognition were not the same. By antibody blocking it was also shown that the antigenic determinant recognized by the autoreactive clone included H2-Ed. The alloreactive cell line therefore contained three H2-E-reactive clonotypes: allo-Ek specific, H2-Ek and DBA/B10 minor plus H2-Ed cross-reactive, and Ed-associated autoreactive.
KW - Animals
KW - Antibodies, Monoclonal
KW - Antigens, Surface
KW - Autoantigens
KW - Binding, Competitive
KW - Cell Line
KW - Clone Cells
KW - Cross Reactions
KW - H-2 Antigens
KW - Haploidy
KW - Histocompatibility Antigens Class II
KW - Lymphocyte Activation
KW - Mice
KW - Mice, Inbred A
KW - Mice, Inbred AKR
KW - Mice, Inbred BALB C
KW - Mice, Inbred C3H
KW - Mice, Inbred C57BL
KW - Mice, Inbred CBA
KW - Mice, Inbred DBA
KW - Minor Histocompatibility Loci
KW - Phenotype
KW - T-Lymphocytes
M3 - Journal article
C2 - 6609002
VL - 85
SP - 436
EP - 446
JO - Cellular Immunology
JF - Cellular Immunology
SN - 0008-8749
IS - 2
ER -