@article{19303770cf0511deb0fe000ea68e967b,
title = "Derivation from an alloreactive T-cell line of a clone which cross-reacts with a self H2-E-restricted minor alloantigen",
abstract = "An alloreactive T-helper-cell line [(A.TH X Balb/c) anti-A.TL] was shown to recognize both H2-Ek and H2-Ed. Both proliferation and polyclonal B-cell activation (protein A plaques) were used in the analyses of specificity. On cloning, the H2-Ek/Ed cross-reaction was shown by one clonotype. This cross-reaction is interpreted in light of the P. Matzinger and M.J. Bevan model (Cell. Immunol. 29, 1, 1977) for antigen recognition by T cells. The antigen recognized was a cell surface non-major histocompatibility (MHC) (minor) alloantigen, shared by the DBA and B10 backgrounds, whose recognition was H2-Ed restricted. This clone is therefore both alloreactive and self-MHC (Ed) restricted minor reactive. Other clones from the cell line were either allospecific or autoreactive. It was shown by antibody blocking that the H2-E alpha (Ia7) determinants involved in the cross-reactive and allospecific recognition were not the same. By antibody blocking it was also shown that the antigenic determinant recognized by the autoreactive clone included H2-Ed. The alloreactive cell line therefore contained three H2-E-reactive clonotypes: allo-Ek specific, H2-Ek and DBA/B10 minor plus H2-Ed cross-reactive, and Ed-associated autoreactive.",
keywords = "Animals, Antibodies, Monoclonal, Antigens, Surface, Autoantigens, Binding, Competitive, Cell Line, Clone Cells, Cross Reactions, H-2 Antigens, Haploidy, Histocompatibility Antigens Class II, Lymphocyte Activation, Mice, Mice, Inbred A, Mice, Inbred AKR, Mice, Inbred BALB C, Mice, Inbred C3H, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Inbred DBA, Minor Histocompatibility Loci, Phenotype, T-Lymphocytes",
author = "T Owens and Liddell, {M E} and Crispe, {I N}",
year = "1984",
language = "English",
volume = "85",
pages = "436--46",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Heinemann",
number = "2",
}