Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark

Linda Harkness, Iben Anne Rasmussen, Karin Erb, Moustapha Kassem

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2010-Mar-3
OriginalsprogEngelsk
TidsskriftIn Vitro Cellular & Developmental Biology - Animal
Vol/bind46
Udgave nummer3-4
Sider (fra-til)259-268
ISSN1071-2690
DOI
StatusUdgivet - 2010

Fingeraftryk

Denmark
Embryonic Structures
Cell Line
Polymerase Chain Reaction
Human Embryonic Stem Cells

Citer dette

@article{4737785037ff11df937d000ea68e967b,
title = "Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark",
abstract = "The derivation and characterisation of human embryonic stem cells provides a source of pluripotent stem cells with potential for clinical applications. Utilising locally sourced embryos from two IVF clinics, we derived and characterised five new cell lines for use in a non-clinical setting. Analysis of clinical data showed that the majority of embryos (94.5{\%}) failed to reach the blastocyst stage of development and of all embryos, regardless of developmental status, 248 embryos were needed to create one stem cell line. From the number of embryos (69) which developed to the blastocyst stage 8.7{\%} developed into cell lines. Using outgrowth of the whole blastocyst, we derived five new, unreported cell lines in Odense, Denmark between 2005 and 2006. Characterisation was carried out using RT-PCR, staining, karyotyping, EB formation and teratoma formation. The KMEB hESC lines will, in the future, be made available through the UK Stem Cell Bank ( http://www.ukstemcellbank.org.uk/ ).",
author = "Linda Harkness and Rasmussen, {Iben Anne} and Karin Erb and Moustapha Kassem",
year = "2010",
doi = "10.1007/s11626-010-9281-7",
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volume = "46",
pages = "259--268",
journal = "In Vitro Cellular & Developmental Biology - Animal",
issn = "1071-2690",
publisher = "Heinemann",
number = "3-4",

}

Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark. / Harkness, Linda; Rasmussen, Iben Anne; Erb, Karin; Kassem, Moustapha.

I: In Vitro Cellular & Developmental Biology - Animal, Bind 46, Nr. 3-4, 2010, s. 259-268.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark

AU - Harkness, Linda

AU - Rasmussen, Iben Anne

AU - Erb, Karin

AU - Kassem, Moustapha

PY - 2010

Y1 - 2010

N2 - The derivation and characterisation of human embryonic stem cells provides a source of pluripotent stem cells with potential for clinical applications. Utilising locally sourced embryos from two IVF clinics, we derived and characterised five new cell lines for use in a non-clinical setting. Analysis of clinical data showed that the majority of embryos (94.5%) failed to reach the blastocyst stage of development and of all embryos, regardless of developmental status, 248 embryos were needed to create one stem cell line. From the number of embryos (69) which developed to the blastocyst stage 8.7% developed into cell lines. Using outgrowth of the whole blastocyst, we derived five new, unreported cell lines in Odense, Denmark between 2005 and 2006. Characterisation was carried out using RT-PCR, staining, karyotyping, EB formation and teratoma formation. The KMEB hESC lines will, in the future, be made available through the UK Stem Cell Bank ( http://www.ukstemcellbank.org.uk/ ).

AB - The derivation and characterisation of human embryonic stem cells provides a source of pluripotent stem cells with potential for clinical applications. Utilising locally sourced embryos from two IVF clinics, we derived and characterised five new cell lines for use in a non-clinical setting. Analysis of clinical data showed that the majority of embryos (94.5%) failed to reach the blastocyst stage of development and of all embryos, regardless of developmental status, 248 embryos were needed to create one stem cell line. From the number of embryos (69) which developed to the blastocyst stage 8.7% developed into cell lines. Using outgrowth of the whole blastocyst, we derived five new, unreported cell lines in Odense, Denmark between 2005 and 2006. Characterisation was carried out using RT-PCR, staining, karyotyping, EB formation and teratoma formation. The KMEB hESC lines will, in the future, be made available through the UK Stem Cell Bank ( http://www.ukstemcellbank.org.uk/ ).

U2 - 10.1007/s11626-010-9281-7

DO - 10.1007/s11626-010-9281-7

M3 - Journal article

C2 - 20198448

VL - 46

SP - 259

EP - 268

JO - In Vitro Cellular & Developmental Biology - Animal

JF - In Vitro Cellular & Developmental Biology - Animal

SN - 1071-2690

IS - 3-4

ER -