Delta-like 1 participates in the specification of ventral midbrain progenitor derived dopaminergic neurons

Matthias Bauer, Jolanta Szulc, Morten Meyer, Charlotte Harken Jensen, Toufik Abbas Terki, Andrea Meixner, Norbert Kinkl, Thomas Gasser, Patrick Aebischer, Marius Ueffing

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

 
Udgivelsesdato: 2008-Feb
OriginalsprogEngelsk
TidsskriftJournal of Neurochemistry
Vol/bind104
Udgave nummer4
Sider (fra-til)1101-15
Antal sider14
ISSN0022-3042
DOI
StatusUdgivet - 1. feb. 2008

Fingeraftryk

Dopaminergic Neurons
Neurons
Specifications
Tyrosine 3-Monooxygenase
Publications
Notch Receptors
In Vitro Techniques

Citer dette

Bauer, Matthias ; Szulc, Jolanta ; Meyer, Morten ; Jensen, Charlotte Harken ; Terki, Toufik Abbas ; Meixner, Andrea ; Kinkl, Norbert ; Gasser, Thomas ; Aebischer, Patrick ; Ueffing, Marius. / Delta-like 1 participates in the specification of ventral midbrain progenitor derived dopaminergic neurons. I: Journal of Neurochemistry. 2008 ; Bind 104, Nr. 4. s. 1101-15.
@article{0290a290fef811dc86ef000ea68e967b,
title = "Delta-like 1 participates in the specification of ventral midbrain progenitor derived dopaminergic neurons",
abstract = "Delta-like 1 (Dlk1), a member of the Delta/Notch protein family, is expressed in the mouse ventral midbrain (VM) as early as embryonic day 11.5 (E11.5) followed by exclusive expression in tyrosine 3-monooxygenase (TH) positive neurons from E12.5 onwards. To further elucidate the yet unknown function of Dlk1 in VM neuron development, we investigated the effect of soluble Dlk1 protein as well as the intrinsic Dlk1 function in the course of VM progenitor expansion and dopaminergic (DA) neuron differentiation in vitro. Dlk1 treatment during expansion increased DA progenitor proliferation and the proportion of NR4A2+ neurons expressing TH after differentiation, whereas Dlk1 treatment during the course of DA precursor differentiation did not alter TH+ neuron counts. In contrast, silencing of endogenously expressed Dlk1 prior to DA precursor differentiation partially prevented the expression of DA neuron markers, which was not accompanied with alteration of overall or local proliferation. Due to the latter finding in combination with the absence of Dlk1 negative DA neurons in differentiated cultures, we suggest that Dlk1 expression might have a permissive effect on DA neuron differentiation in vitro. The study presented here is the first publication identifying Dlk1 effects on ventral midbrain-derived DA precursor differentiation.",
keywords = "Animals, Cell Differentiation, Cell Proliferation, Cells, Cultured, Dopamine, Female, Humans, Intracellular Signaling Peptides and Proteins, Membrane Proteins, Mesencephalon, Mice, NIH 3T3 Cells, Neurons, Pregnancy, Stem Cells",
author = "Matthias Bauer and Jolanta Szulc and Morten Meyer and Jensen, {Charlotte Harken} and Terki, {Toufik Abbas} and Andrea Meixner and Norbert Kinkl and Thomas Gasser and Patrick Aebischer and Marius Ueffing",
year = "2008",
month = "2",
day = "1",
doi = "10.1111/j.1471-4159.2007.05037.x",
language = "English",
volume = "104",
pages = "1101--15",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "4",

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Delta-like 1 participates in the specification of ventral midbrain progenitor derived dopaminergic neurons. / Bauer, Matthias; Szulc, Jolanta; Meyer, Morten; Jensen, Charlotte Harken; Terki, Toufik Abbas; Meixner, Andrea; Kinkl, Norbert; Gasser, Thomas; Aebischer, Patrick; Ueffing, Marius.

I: Journal of Neurochemistry, Bind 104, Nr. 4, 01.02.2008, s. 1101-15.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Delta-like 1 participates in the specification of ventral midbrain progenitor derived dopaminergic neurons

AU - Bauer, Matthias

AU - Szulc, Jolanta

AU - Meyer, Morten

AU - Jensen, Charlotte Harken

AU - Terki, Toufik Abbas

AU - Meixner, Andrea

AU - Kinkl, Norbert

AU - Gasser, Thomas

AU - Aebischer, Patrick

AU - Ueffing, Marius

PY - 2008/2/1

Y1 - 2008/2/1

N2 - Delta-like 1 (Dlk1), a member of the Delta/Notch protein family, is expressed in the mouse ventral midbrain (VM) as early as embryonic day 11.5 (E11.5) followed by exclusive expression in tyrosine 3-monooxygenase (TH) positive neurons from E12.5 onwards. To further elucidate the yet unknown function of Dlk1 in VM neuron development, we investigated the effect of soluble Dlk1 protein as well as the intrinsic Dlk1 function in the course of VM progenitor expansion and dopaminergic (DA) neuron differentiation in vitro. Dlk1 treatment during expansion increased DA progenitor proliferation and the proportion of NR4A2+ neurons expressing TH after differentiation, whereas Dlk1 treatment during the course of DA precursor differentiation did not alter TH+ neuron counts. In contrast, silencing of endogenously expressed Dlk1 prior to DA precursor differentiation partially prevented the expression of DA neuron markers, which was not accompanied with alteration of overall or local proliferation. Due to the latter finding in combination with the absence of Dlk1 negative DA neurons in differentiated cultures, we suggest that Dlk1 expression might have a permissive effect on DA neuron differentiation in vitro. The study presented here is the first publication identifying Dlk1 effects on ventral midbrain-derived DA precursor differentiation.

AB - Delta-like 1 (Dlk1), a member of the Delta/Notch protein family, is expressed in the mouse ventral midbrain (VM) as early as embryonic day 11.5 (E11.5) followed by exclusive expression in tyrosine 3-monooxygenase (TH) positive neurons from E12.5 onwards. To further elucidate the yet unknown function of Dlk1 in VM neuron development, we investigated the effect of soluble Dlk1 protein as well as the intrinsic Dlk1 function in the course of VM progenitor expansion and dopaminergic (DA) neuron differentiation in vitro. Dlk1 treatment during expansion increased DA progenitor proliferation and the proportion of NR4A2+ neurons expressing TH after differentiation, whereas Dlk1 treatment during the course of DA precursor differentiation did not alter TH+ neuron counts. In contrast, silencing of endogenously expressed Dlk1 prior to DA precursor differentiation partially prevented the expression of DA neuron markers, which was not accompanied with alteration of overall or local proliferation. Due to the latter finding in combination with the absence of Dlk1 negative DA neurons in differentiated cultures, we suggest that Dlk1 expression might have a permissive effect on DA neuron differentiation in vitro. The study presented here is the first publication identifying Dlk1 effects on ventral midbrain-derived DA precursor differentiation.

KW - Animals

KW - Cell Differentiation

KW - Cell Proliferation

KW - Cells, Cultured

KW - Dopamine

KW - Female

KW - Humans

KW - Intracellular Signaling Peptides and Proteins

KW - Membrane Proteins

KW - Mesencephalon

KW - Mice

KW - NIH 3T3 Cells

KW - Neurons

KW - Pregnancy

KW - Stem Cells

U2 - 10.1111/j.1471-4159.2007.05037.x

DO - 10.1111/j.1471-4159.2007.05037.x

M3 - Journal article

VL - 104

SP - 1101

EP - 1115

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 4

ER -