Defining the diffusion in model membranes using line fluorescence recovery after photobleaching

Jakob L. Kure; Camilla B. Andersen; Thomas E. Rasmussen; B. Christoffer Lagerholm; Eva C. Arnspang

doi:10.3390/membranes10120434

Defining the diffusion in model membranes using line fluorescence recovery after photobleaching

Jakob L. Kure, Camilla B. Andersen, Thomas E. Rasmussen, B. Christoffer Lagerholm, Eva C. Arnspang^*

^*Kontaktforfatter

University of Oxford

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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Abstrakt

In this study, we explore the use of line FRAP to detect diffusion in synthetic lipid membranes. The study of the dynamics of these membrane lipids can, however, be challenging. The diffusion in two different synthetic membranes consisting of the lipid mixtures 1:1 DOPC:DPPC and 2:2:1 DOPC:DPPC:Cholesterol was studied with line FRAP. A correlation between diffusion coefficient and temperature was found to be dependent on the morphology of the membrane. We suggest line FRAP as a promising accessible and simple technique to study diffusion in plasma membranes.

Originalsprog	Engelsk
Artikelnummer	434
Tidsskrift	Membranes
Vol/bind	10
Udgave nummer	12
Antal sider	7
ISSN	2077-0375
DOI	https://doi.org/10.3390/membranes10120434
Status	Udgivet - 17. dec. 2020

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10.3390/membranes10120434Licens: CC BY

Open Access VersionForlagets udgivne version, 984 KBLicens: CC BY

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https://www.mdpi.com/2077-0375/10/12/434

1 Ph.d.-afhandling

Imaging Nano-domains in Mammalian Membranes Using Super-Resolution Microscopy and Advanced Analysis
Lavrsen Kure, J., 13. dec. 2021, Syddansk Universitet. Det Tekniske Fakultet. 137 s.
Publikation: Afhandling › Ph.d.-afhandling

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Citationsformater

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title = "Defining the diffusion in model membranes using line fluorescence recovery after photobleaching",

abstract = "In this study, we explore the use of line FRAP to detect diffusion in synthetic lipid membranes. The study of the dynamics of these membrane lipids can, however, be challenging. The diffusion in two different synthetic membranes consisting of the lipid mixtures 1:1 DOPC:DPPC and 2:2:1 DOPC:DPPC:Cholesterol was studied with line FRAP. A correlation between diffusion coefficient and temperature was found to be dependent on the morphology of the membrane. We suggest line FRAP as a promising accessible and simple technique to study diffusion in plasma membranes.",

keywords = "Diffusion, Line FRAP, Microscopy, Plasma membrane lipids, Synthetic membrane",

author = "Kure, {Jakob L.} and Andersen, {Camilla B.} and Rasmussen, {Thomas E.} and Lagerholm, {B. Christoffer} and Arnspang, {Eva C.}",

year = "2020",

month = dec,

day = "17",

doi = "10.3390/membranes10120434",

language = "English",

volume = "10",

journal = "Membranes",

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TY - JOUR

T1 - Defining the diffusion in model membranes using line fluorescence recovery after photobleaching

AU - Kure, Jakob L.

AU - Andersen, Camilla B.

AU - Rasmussen, Thomas E.

AU - Lagerholm, B. Christoffer

AU - Arnspang, Eva C.

PY - 2020/12/17

Y1 - 2020/12/17

N2 - In this study, we explore the use of line FRAP to detect diffusion in synthetic lipid membranes. The study of the dynamics of these membrane lipids can, however, be challenging. The diffusion in two different synthetic membranes consisting of the lipid mixtures 1:1 DOPC:DPPC and 2:2:1 DOPC:DPPC:Cholesterol was studied with line FRAP. A correlation between diffusion coefficient and temperature was found to be dependent on the morphology of the membrane. We suggest line FRAP as a promising accessible and simple technique to study diffusion in plasma membranes.

AB - In this study, we explore the use of line FRAP to detect diffusion in synthetic lipid membranes. The study of the dynamics of these membrane lipids can, however, be challenging. The diffusion in two different synthetic membranes consisting of the lipid mixtures 1:1 DOPC:DPPC and 2:2:1 DOPC:DPPC:Cholesterol was studied with line FRAP. A correlation between diffusion coefficient and temperature was found to be dependent on the morphology of the membrane. We suggest line FRAP as a promising accessible and simple technique to study diffusion in plasma membranes.

KW - Diffusion

KW - Line FRAP

KW - Microscopy

KW - Plasma membrane lipids

KW - Synthetic membrane

UR - https://www.mdpi.com/2077-0375/10/12/434

U2 - 10.3390/membranes10120434

DO - 10.3390/membranes10120434

M3 - Journal article

C2 - 33348780

AN - SCOPUS:85097828783

VL - 10

JO - Membranes

JF - Membranes

SN - 2077-0375

IS - 12

M1 - 434

ER -

Defining the diffusion in model membranes using line fluorescence recovery after photobleaching

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Imaging Nano-domains in Mammalian Membranes Using Super-Resolution Microscopy and Advanced Analysis

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