Decellularized matrix from tumorigenic human mesenchymal stem cells promotes neovascularization with galectin-1 dependent endothelial interaction

Jorge S Burns, Malthe Kristiansen, Lars P Kristensen, Kenneth H Larsen, Maria O Nielsen, Helle Christiansen, Jan Nehlin, Jens S. Andersen, Moustapha Kassem

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Acquisition of a blood supply is fundamental for extensive tumor growth. We recently described vascular heterogeneity in tumours derived from cell clones of a human mesenchymal stem cell (hMSC) strain (hMSC-TERT20) immortalized by retroviral vector mediated human telomerase (hTERT) gene expression. Histological analysis showed that cells of the most vascularized tumorigenic clone, -BD11 had a pericyte-like alpha smooth muscle actin (ASMA+) and CD146+ positive phenotype. Upon serum withdrawal in culture, -BD11 cells formed cord-like structures mimicking capillary morphogenesis. In contrast, cells of the poorly tumorigenic clone, -BC8 did not stain for ASMA, tumours were less vascularized and serum withdrawal in culture led to cell death. By exploring the heterogeneity in hMSC-TERT20 clones we aimed to understand molecular mechanisms by which mesenchymal stem cells may promote neovascularization.
OriginalsprogEngelsk
TidsskriftP L o S One
Vol/bind6
Udgave nummer7
Sider (fra-til)e21888
ISSN1932-6203
DOI
StatusUdgivet - 1. jan. 2011

Fingeraftryk

Galectin 1
angiogenesis
Stem cells
Mesenchymal Stromal Cells
stem cells
Clone Cells
clones
Tumors
blood serum
Cell culture
neoplasms
Clone cells
retroviral vectors
Neoplasms
telomerase
Pericytes
Telomerase
Cell death
cells
Serum

Citer dette

Burns, Jorge S ; Kristiansen, Malthe ; Kristensen, Lars P ; Larsen, Kenneth H ; Nielsen, Maria O ; Christiansen, Helle ; Nehlin, Jan ; Andersen, Jens S. ; Kassem, Moustapha. / Decellularized matrix from tumorigenic human mesenchymal stem cells promotes neovascularization with galectin-1 dependent endothelial interaction. I: P L o S One. 2011 ; Bind 6, Nr. 7. s. e21888.
@article{75f51ecdd5cc4489aeecb35128a19837,
title = "Decellularized matrix from tumorigenic human mesenchymal stem cells promotes neovascularization with galectin-1 dependent endothelial interaction",
abstract = "Acquisition of a blood supply is fundamental for extensive tumor growth. We recently described vascular heterogeneity in tumours derived from cell clones of a human mesenchymal stem cell (hMSC) strain (hMSC-TERT20) immortalized by retroviral vector mediated human telomerase (hTERT) gene expression. Histological analysis showed that cells of the most vascularized tumorigenic clone, -BD11 had a pericyte-like alpha smooth muscle actin (ASMA+) and CD146+ positive phenotype. Upon serum withdrawal in culture, -BD11 cells formed cord-like structures mimicking capillary morphogenesis. In contrast, cells of the poorly tumorigenic clone, -BC8 did not stain for ASMA, tumours were less vascularized and serum withdrawal in culture led to cell death. By exploring the heterogeneity in hMSC-TERT20 clones we aimed to understand molecular mechanisms by which mesenchymal stem cells may promote neovascularization.",
author = "Burns, {Jorge S} and Malthe Kristiansen and Kristensen, {Lars P} and Larsen, {Kenneth H} and Nielsen, {Maria O} and Helle Christiansen and Jan Nehlin and Andersen, {Jens S.} and Moustapha Kassem",
year = "2011",
month = "1",
day = "1",
doi = "10.1371/journal.pone.0021888",
language = "English",
volume = "6",
pages = "e21888",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "7",

}

Decellularized matrix from tumorigenic human mesenchymal stem cells promotes neovascularization with galectin-1 dependent endothelial interaction. / Burns, Jorge S; Kristiansen, Malthe; Kristensen, Lars P; Larsen, Kenneth H; Nielsen, Maria O; Christiansen, Helle; Nehlin, Jan; Andersen, Jens S.; Kassem, Moustapha.

I: P L o S One, Bind 6, Nr. 7, 01.01.2011, s. e21888.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Decellularized matrix from tumorigenic human mesenchymal stem cells promotes neovascularization with galectin-1 dependent endothelial interaction

AU - Burns, Jorge S

AU - Kristiansen, Malthe

AU - Kristensen, Lars P

AU - Larsen, Kenneth H

AU - Nielsen, Maria O

AU - Christiansen, Helle

AU - Nehlin, Jan

AU - Andersen, Jens S.

AU - Kassem, Moustapha

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Acquisition of a blood supply is fundamental for extensive tumor growth. We recently described vascular heterogeneity in tumours derived from cell clones of a human mesenchymal stem cell (hMSC) strain (hMSC-TERT20) immortalized by retroviral vector mediated human telomerase (hTERT) gene expression. Histological analysis showed that cells of the most vascularized tumorigenic clone, -BD11 had a pericyte-like alpha smooth muscle actin (ASMA+) and CD146+ positive phenotype. Upon serum withdrawal in culture, -BD11 cells formed cord-like structures mimicking capillary morphogenesis. In contrast, cells of the poorly tumorigenic clone, -BC8 did not stain for ASMA, tumours were less vascularized and serum withdrawal in culture led to cell death. By exploring the heterogeneity in hMSC-TERT20 clones we aimed to understand molecular mechanisms by which mesenchymal stem cells may promote neovascularization.

AB - Acquisition of a blood supply is fundamental for extensive tumor growth. We recently described vascular heterogeneity in tumours derived from cell clones of a human mesenchymal stem cell (hMSC) strain (hMSC-TERT20) immortalized by retroviral vector mediated human telomerase (hTERT) gene expression. Histological analysis showed that cells of the most vascularized tumorigenic clone, -BD11 had a pericyte-like alpha smooth muscle actin (ASMA+) and CD146+ positive phenotype. Upon serum withdrawal in culture, -BD11 cells formed cord-like structures mimicking capillary morphogenesis. In contrast, cells of the poorly tumorigenic clone, -BC8 did not stain for ASMA, tumours were less vascularized and serum withdrawal in culture led to cell death. By exploring the heterogeneity in hMSC-TERT20 clones we aimed to understand molecular mechanisms by which mesenchymal stem cells may promote neovascularization.

U2 - 10.1371/journal.pone.0021888

DO - 10.1371/journal.pone.0021888

M3 - Journal article

C2 - 21779348

VL - 6

SP - e21888

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 7

ER -