Breastfed infants have higher intestinal lipid absorption and neurodevelopmental outcomes compared to formula-fed infants, which may relate to a different surface layer structure of fat globules in infant formula. This study investigated if dairy-derived emulsifiers increased lipid absorption and neurodevelopment relative to soy lecithin in newborn preterm piglets. Piglets received a formula diet containing soy lecithin (SL) or whey protein concentrate enriched in extracellular vesicles (WPC-A-EV) or phospholipids (WPC-PL) for 19 days. Both WPC-A-EV and WPC-PL emulsions, but not the intact diets, increased in vitro lipolysis compared to SL. The main differences of plasma lipidomics analysis were increased levels of some sphingolipids, and lipid molecules with odd-chain (17:1, 19:1, 19:3) as well as mono-and polyunsaturated fatty acyl chains (16:1, 20:1, 20:3) in the WPC-A-EV and WPC-PL groups and increased 18:2 fatty acyls in the SL group. Indirect monitoring of intestinal triacylglycerol absorption showed no differences between groups. Diffusor tensor imaging measurements of mean diffusivity in the hippocampus were lower for WPC-A-EV and WPC-PL groups compared to SL indicating improved hippocampal maturation. No differences in hippocampal lipid composition or short-term memory were observed between groups. In conclu-sion, emulsification of fat globules in infant formula with dairy-derived emulsifiers altered the plasma lipid profile and hippocampal tissue diffusivity but had limited effects on other absorptive and learning abilities relative to SL in preterm piglets.
Bibliografisk noteFunding Information:
Funding: This research was funded by the Innovation Fund Denmark (Grant no.: 5158-00014B), the Dairy Research Foundation and Arla Foods Ingredients Denmark.
This research was funded by the Innovation Fund Denmark (Grant no.: 5158-00014B), the Dairy Research Foundation and Arla Foods Ingredients Denmark. The authors would like to thank Britta Karlsson, Elin Skytte, Jane Povlsen, Ben-jamin Lambert-Z?berlein and Mette Ernst for their assistance in experimental animal work. Moreo-ver, we thank Kenneth Olsson, Ann-Dorit Moltke S?rensen, Charlotte Jacobsen and Lis Berner for their assistance in producing the emulsions. We also thank Yuki Mori and the Panum Preclinical MRI Core Facility for conducting the MRI scans. The late Lars Ingvar Hellgren, Technical University of Denmark, is acknowledged for his passionate initiation and steering of the InfantBrain project until his health condition prevented further involvement.
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