Culture confirmation of gonococcal infection by recall of subjects found to be positive by nucleic acid amplification tests in general practice

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

OBJECTIVES: To evaluate a routine notification of general practitioners to recall nucleic acid amplification test (NAAT)-positive subjects for culture of Neisseria gonorrhoeae to confirm gonococcal infection in the community.

METHODS: A retrospective observational study of the routine testing for N gonorrhoeae by analysis of test results compiled from the laboratory information system in two departments of clinical microbiology.

RESULTS: Altogether, 158 male and female subjects with NAAT-positive results for N gonorrhoeae were included in the study. Samples for culture of N gonorrhoeae were collected from 102/158 (64.6%) subjects recalled after a NAAT assay was found positive. Growth of N gonorrhoeae was seen in the samples from 54/102 (52.9%) of the re-examined NAAT-positive subjects. Among subjects with samples collected within the first week after the positive NAAT test, 34/44 (77.3%) were confirmed positive by culture.

CONCLUSION: This study shows that it is possible for the general practitioner to recall a substantial number of NAAT-positive subjects to collect swabs for culture of N gonorrhoeae to confirm gonococcal infection in the community. Most recall samples are culture positive if collected within a week of the NAAT-positive test, and may provide a sufficient monitoring of the drug susceptibility of N gonorrhoeae strains in the community.

OriginalsprogEngelsk
TidsskriftSexually Transmitted Infections
Vol/bind86
Udgave nummer6
Sider (fra-til)478-9
Antal sider2
ISSN1368-4973
DOI
StatusUdgivet - 1. nov. 2010

Fingeraftryk

Nucleic Acid Amplification Techniques
Neisseria gonorrhoeae
General Practice
General Practitioners

Emneord

  • Neisseria gonorrhoeae
  • NAAT
  • Culture
  • recall samples

Citer dette

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title = "Culture confirmation of gonococcal infection by recall of subjects found to be positive by nucleic acid amplification tests in general practice",
abstract = "To evaluate a routine notification of general practitioners to recall nucleic acid amplification test (NAAT)-positive subjects for culture of Neisseria gonorrhoeae to confirm gonococcal infection in the community.",
keywords = "Appointments and Schedules, Bacteriological Techniques, Cell Culture Techniques, Denmark, Family Practice, Female, Gonorrhea, Humans, Male, Neisseria gonorrhoeae, Nucleic Acid Amplification Techniques, Polymerase Chain Reaction, Retrospective Studies, Specimen Handling, Neisseria gonorrhoeae, NAAT, Culture, recall samples",
author = "M{\o}ller, {Jens Kj{\o}lseth}",
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Culture confirmation of gonococcal infection by recall of subjects found to be positive by nucleic acid amplification tests in general practice. / Møller, Jens Kjølseth.

I: Sexually Transmitted Infections, Bind 86, Nr. 6, 01.11.2010, s. 478-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Culture confirmation of gonococcal infection by recall of subjects found to be positive by nucleic acid amplification tests in general practice

AU - Møller, Jens Kjølseth

PY - 2010/11/1

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N2 - To evaluate a routine notification of general practitioners to recall nucleic acid amplification test (NAAT)-positive subjects for culture of Neisseria gonorrhoeae to confirm gonococcal infection in the community.

AB - To evaluate a routine notification of general practitioners to recall nucleic acid amplification test (NAAT)-positive subjects for culture of Neisseria gonorrhoeae to confirm gonococcal infection in the community.

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KW - Cell Culture Techniques

KW - Denmark

KW - Family Practice

KW - Female

KW - Gonorrhea

KW - Humans

KW - Male

KW - Neisseria gonorrhoeae

KW - Nucleic Acid Amplification Techniques

KW - Polymerase Chain Reaction

KW - Retrospective Studies

KW - Specimen Handling

KW - Neisseria gonorrhoeae

KW - NAAT

KW - Culture

KW - recall samples

U2 - 10.1136/sti.2010.043703

DO - 10.1136/sti.2010.043703

M3 - Journal article

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SP - 478

EP - 479

JO - Sexually Transmitted Infections

JF - Sexually Transmitted Infections

SN - 1368-4973

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