Crystallization of Steviol Glycosides from Stevia rebaudiana Extracts

Chandrakant Ramkrishna Malwade, Rime El-Houri, Haiyan Qu

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskningpeer review

Resumé

Recovery of steviol glycosides, known for their sweetness as well as therapeutic properties, from dried leaves of the plant Stevia rebaudiana is investigated in this work. Firstly, the steviol glycosides were isolated from the dried leaves by the extraction with solvents ethyl acetate, methanol, ethanol, water and ethanol – water mixtures at room temperature. The yield and composition of the extract was clearly affected by the solvent with highest yield observed in water. Seeded and unseeded cooling crystallization of glycosides directly from the extracts did not yield any crystals indicating the strong influence of impurities on crystallization. In further investigations, methanol extract was fractionated with column chromatography and cooling crystallization of individual fractions yielded crystals of stevioside (96% purity). However, the yield of the process was very low, which needs to be optimized further.
OriginalsprogEngelsk
Publikationsdato5. sep. 2018
StatusUdgivet - 5. sep. 2018
BegivenhedBIWIC 2018: 25th International Workshop on Industrial Crystallization - University of Rouen, Rouen, Frankrig
Varighed: 5. sep. 20187. sep. 2018

Konference

KonferenceBIWIC 2018
LokationUniversity of Rouen
LandFrankrig
ByRouen
Periode05/09/201807/09/2018

Fingeraftryk

steviol
Stevia rebaudiana
crystallization
glycosides
extracts
crystals
methanol
cooling
ethanol
stevioside
medicinal properties
water
sweetness
purity
leaves
ambient temperature
chromatography

Citer dette

Malwade, C. R., El-Houri, R., & Qu, H. (2018). Crystallization of Steviol Glycosides from Stevia rebaudiana Extracts. Poster session præsenteret på BIWIC 2018, Rouen, Frankrig.
Malwade, Chandrakant Ramkrishna ; El-Houri, Rime ; Qu, Haiyan. / Crystallization of Steviol Glycosides from Stevia rebaudiana Extracts. Poster session præsenteret på BIWIC 2018, Rouen, Frankrig.
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title = "Crystallization of Steviol Glycosides from Stevia rebaudiana Extracts",
abstract = "Recovery of steviol glycosides, known for their sweetness as well as therapeutic properties, from dried leaves of the plant Stevia rebaudiana is investigated in this work. Firstly, the steviol glycosides were isolated from the dried leaves by the extraction with solvents ethyl acetate, methanol, ethanol, water and ethanol – water mixtures at room temperature. The yield and composition of the extract was clearly affected by the solvent with highest yield observed in water. Seeded and unseeded cooling crystallization of glycosides directly from the extracts did not yield any crystals indicating the strong influence of impurities on crystallization. In further investigations, methanol extract was fractionated with column chromatography and cooling crystallization of individual fractions yielded crystals of stevioside (96{\%} purity). However, the yield of the process was very low, which needs to be optimized further.",
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Malwade, CR, El-Houri, R & Qu, H 2018, 'Crystallization of Steviol Glycosides from Stevia rebaudiana Extracts' BIWIC 2018, Rouen, Frankrig, 05/09/2018 - 07/09/2018, .

Crystallization of Steviol Glycosides from Stevia rebaudiana Extracts. / Malwade, Chandrakant Ramkrishna; El-Houri, Rime; Qu, Haiyan.

2018. Poster session præsenteret på BIWIC 2018, Rouen, Frankrig.

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskningpeer review

TY - CONF

T1 - Crystallization of Steviol Glycosides from Stevia rebaudiana Extracts

AU - Malwade, Chandrakant Ramkrishna

AU - El-Houri, Rime

AU - Qu, Haiyan

PY - 2018/9/5

Y1 - 2018/9/5

N2 - Recovery of steviol glycosides, known for their sweetness as well as therapeutic properties, from dried leaves of the plant Stevia rebaudiana is investigated in this work. Firstly, the steviol glycosides were isolated from the dried leaves by the extraction with solvents ethyl acetate, methanol, ethanol, water and ethanol – water mixtures at room temperature. The yield and composition of the extract was clearly affected by the solvent with highest yield observed in water. Seeded and unseeded cooling crystallization of glycosides directly from the extracts did not yield any crystals indicating the strong influence of impurities on crystallization. In further investigations, methanol extract was fractionated with column chromatography and cooling crystallization of individual fractions yielded crystals of stevioside (96% purity). However, the yield of the process was very low, which needs to be optimized further.

AB - Recovery of steviol glycosides, known for their sweetness as well as therapeutic properties, from dried leaves of the plant Stevia rebaudiana is investigated in this work. Firstly, the steviol glycosides were isolated from the dried leaves by the extraction with solvents ethyl acetate, methanol, ethanol, water and ethanol – water mixtures at room temperature. The yield and composition of the extract was clearly affected by the solvent with highest yield observed in water. Seeded and unseeded cooling crystallization of glycosides directly from the extracts did not yield any crystals indicating the strong influence of impurities on crystallization. In further investigations, methanol extract was fractionated with column chromatography and cooling crystallization of individual fractions yielded crystals of stevioside (96% purity). However, the yield of the process was very low, which needs to be optimized further.

M3 - Poster

ER -

Malwade CR, El-Houri R, Qu H. Crystallization of Steviol Glycosides from Stevia rebaudiana Extracts. 2018. Poster session præsenteret på BIWIC 2018, Rouen, Frankrig.