Correlation between molecular genetic analyses and immunohistochemical evaluation of the epidermal growth factor receptor and p185(HER2)

Anne Marie Gerdes*, Ole Nielsen, Ulla Mohr, Per Pfeifper, Ann Knoop, Carsten Rose, Mogens Hørder, Per Prætorius Clausen

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Several methods have been developed for the measurement of gene amplification and expression. This study compared different molecular genetic analyses (Southern blot analysis (SBA) and polymerase chain reaction (PCR)) with immunohistochemical (IHC) evaluation of the coresponding protein content. PCR may be used as a semi-quantitative analysis of gene amplification and allows DNA extraction from paraffin-embedded blocks. SBA is more accurate than PCR to measure the exact degree of amplification, but only DNA extracted from frozen or fresh tissue can by used. We examined 23 breast tumors and 16 lung tumors, where the genes HER-1 coding for the epidermal growth factor receptor (EGFR) and HER-2 coding for p185(HER-2) were analysed. Furthermore, PCR performed on DNA from frozen tissue was compared to PCR on DNA extracted from paraffin-embedded blocks. The results showed correlation between the different analyses, especially when the gene copy number was highly amplified. Some breast tumors showed moderately increased gene copy number of HER-1 by SBA, but no increased protein content by IHC evaluation. This probably reflects that minor degrees of genetic aberrations are not sufficient to cause major biological disturbances, because regulatory cellular pathways are still operating.

OriginalsprogEngelsk
TidsskriftAnticancer Research
Vol/bind18
Udgave nummer4 A
Sider (fra-til)2529-2534
Antal sider6
ISSN0250-7005
StatusUdgivet - 1. jul. 1998

Fingeraftryk

Epidermal Growth Factor Receptor
Polymerase Chain Reaction
Southern Blotting
Gene Amplification
DNA
Paraffin
Proteins
Lung
Neoplasms

Citer dette

Gerdes, A. M., Nielsen, O., Mohr, U., Pfeifper, P., Knoop, A., Rose, C., ... Clausen, P. P. (1998). Correlation between molecular genetic analyses and immunohistochemical evaluation of the epidermal growth factor receptor and p185(HER2). Anticancer Research, 18(4 A), 2529-2534.
Gerdes, Anne Marie ; Nielsen, Ole ; Mohr, Ulla ; Pfeifper, Per ; Knoop, Ann ; Rose, Carsten ; Hørder, Mogens ; Clausen, Per Prætorius. / Correlation between molecular genetic analyses and immunohistochemical evaluation of the epidermal growth factor receptor and p185(HER2). I: Anticancer Research. 1998 ; Bind 18, Nr. 4 A. s. 2529-2534.
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title = "Correlation between molecular genetic analyses and immunohistochemical evaluation of the epidermal growth factor receptor and p185(HER2)",
abstract = "Several methods have been developed for the measurement of gene amplification and expression. This study compared different molecular genetic analyses (Southern blot analysis (SBA) and polymerase chain reaction (PCR)) with immunohistochemical (IHC) evaluation of the coresponding protein content. PCR may be used as a semi-quantitative analysis of gene amplification and allows DNA extraction from paraffin-embedded blocks. SBA is more accurate than PCR to measure the exact degree of amplification, but only DNA extracted from frozen or fresh tissue can by used. We examined 23 breast tumors and 16 lung tumors, where the genes HER-1 coding for the epidermal growth factor receptor (EGFR) and HER-2 coding for p185(HER-2) were analysed. Furthermore, PCR performed on DNA from frozen tissue was compared to PCR on DNA extracted from paraffin-embedded blocks. The results showed correlation between the different analyses, especially when the gene copy number was highly amplified. Some breast tumors showed moderately increased gene copy number of HER-1 by SBA, but no increased protein content by IHC evaluation. This probably reflects that minor degrees of genetic aberrations are not sufficient to cause major biological disturbances, because regulatory cellular pathways are still operating.",
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Gerdes, AM, Nielsen, O, Mohr, U, Pfeifper, P, Knoop, A, Rose, C, Hørder, M & Clausen, PP 1998, 'Correlation between molecular genetic analyses and immunohistochemical evaluation of the epidermal growth factor receptor and p185(HER2)', Anticancer Research, bind 18, nr. 4 A, s. 2529-2534.

Correlation between molecular genetic analyses and immunohistochemical evaluation of the epidermal growth factor receptor and p185(HER2). / Gerdes, Anne Marie; Nielsen, Ole; Mohr, Ulla; Pfeifper, Per; Knoop, Ann; Rose, Carsten; Hørder, Mogens; Clausen, Per Prætorius.

I: Anticancer Research, Bind 18, Nr. 4 A, 01.07.1998, s. 2529-2534.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Correlation between molecular genetic analyses and immunohistochemical evaluation of the epidermal growth factor receptor and p185(HER2)

AU - Gerdes, Anne Marie

AU - Nielsen, Ole

AU - Mohr, Ulla

AU - Pfeifper, Per

AU - Knoop, Ann

AU - Rose, Carsten

AU - Hørder, Mogens

AU - Clausen, Per Prætorius

PY - 1998/7/1

Y1 - 1998/7/1

N2 - Several methods have been developed for the measurement of gene amplification and expression. This study compared different molecular genetic analyses (Southern blot analysis (SBA) and polymerase chain reaction (PCR)) with immunohistochemical (IHC) evaluation of the coresponding protein content. PCR may be used as a semi-quantitative analysis of gene amplification and allows DNA extraction from paraffin-embedded blocks. SBA is more accurate than PCR to measure the exact degree of amplification, but only DNA extracted from frozen or fresh tissue can by used. We examined 23 breast tumors and 16 lung tumors, where the genes HER-1 coding for the epidermal growth factor receptor (EGFR) and HER-2 coding for p185(HER-2) were analysed. Furthermore, PCR performed on DNA from frozen tissue was compared to PCR on DNA extracted from paraffin-embedded blocks. The results showed correlation between the different analyses, especially when the gene copy number was highly amplified. Some breast tumors showed moderately increased gene copy number of HER-1 by SBA, but no increased protein content by IHC evaluation. This probably reflects that minor degrees of genetic aberrations are not sufficient to cause major biological disturbances, because regulatory cellular pathways are still operating.

AB - Several methods have been developed for the measurement of gene amplification and expression. This study compared different molecular genetic analyses (Southern blot analysis (SBA) and polymerase chain reaction (PCR)) with immunohistochemical (IHC) evaluation of the coresponding protein content. PCR may be used as a semi-quantitative analysis of gene amplification and allows DNA extraction from paraffin-embedded blocks. SBA is more accurate than PCR to measure the exact degree of amplification, but only DNA extracted from frozen or fresh tissue can by used. We examined 23 breast tumors and 16 lung tumors, where the genes HER-1 coding for the epidermal growth factor receptor (EGFR) and HER-2 coding for p185(HER-2) were analysed. Furthermore, PCR performed on DNA from frozen tissue was compared to PCR on DNA extracted from paraffin-embedded blocks. The results showed correlation between the different analyses, especially when the gene copy number was highly amplified. Some breast tumors showed moderately increased gene copy number of HER-1 by SBA, but no increased protein content by IHC evaluation. This probably reflects that minor degrees of genetic aberrations are not sufficient to cause major biological disturbances, because regulatory cellular pathways are still operating.

KW - EGFreceptor

KW - Immunohistochemistry

KW - p185(HER-2)

KW - PCR

KW - Southern blot

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M3 - Journal article

C2 - 9703905

AN - SCOPUS:0031840082

VL - 18

SP - 2529

EP - 2534

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 4 A

ER -