Copper transfer from Cu-Aβ to human serum albumin inhibits aggregation, radical production and reduces Aβ toxicity

Lorena Perrone, Emmanuelle Mothes, Maeva Vignes, Anaïs Mockel, Cindy Figueroa, Marie Christine Miquel, Marie Lise Maddelein, Peter Faller*

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Amyloid-β peptides (Aβ) and the protein human serum albumin (HSA) interact in vivo. They are both localised in the blood plasma and in the cerebrospinal fluid. Among other functions, HSA is involved in the transport of the essential metal copper. Complexes between Aβ and copper ions have been proposed to be an aberrant interaction implicated in the development of Alzheimer's disease, where Cu is involved in Aβ aggregation and production of reactive oxygen species (ROS). In the present work, we studied copper-exchange reaction between Aβ and HSA or the tetrapeptide DAHK (N-terminal Cu-binding domain of HSA) and the consequence of this exchange on Aβ-induced ROS production and cell toxicity. The following results were obtained: 1) HSA and DAHK removed CuII from Ab rapidly and stoichiometrically, 2) HSA and DAHK were able to decrease Cu-induced aggregation of Aβ, 3) HSA and DAHK suppressed the catalytic HO· production in vitro and ROS production in neuroblastoma cells generated by Cu-Aβ and ascorbate, 4) HSA and DAHK were able to rescue these cells from the toxicity of Cu-Aβ with ascorbate, 5) DAHK was more potent in ROS suppression and restoration of neuroblastoma cell viability than HSA, in correlation with an easier reduction of CuII-HSA than Cu-DAHK by ascorbate, in vitro. Our data suggest that HSA is able to decrease aberrant CuII-Aβ interaction. The repercussion of the competition between HSA and Ab to bind Cu in the blood and brain and its relation to Alzheimer's disease are discussed.

OriginalsprogEngelsk
TidsskriftChemBioChem
Vol/bind11
Udgave nummer1
Sider (fra-til)110-118
Antal sider9
ISSN1439-4227
DOI
StatusUdgivet - 4. jan. 2010

Fingeraftryk

Serum Albumin
Toxicity
Copper
Agglomeration
Reactive Oxygen Species
Neuroblastoma
Alzheimer Disease
Blood
Cerebrospinal fluid
Amyloid
Restoration
Cerebrospinal Fluid
Brain
Cell Survival
Metals
Cells
Ions
Plasmas

Citer dette

Perrone, Lorena ; Mothes, Emmanuelle ; Vignes, Maeva ; Mockel, Anaïs ; Figueroa, Cindy ; Miquel, Marie Christine ; Maddelein, Marie Lise ; Faller, Peter. / Copper transfer from Cu-Aβ to human serum albumin inhibits aggregation, radical production and reduces Aβ toxicity. I: ChemBioChem. 2010 ; Bind 11, Nr. 1. s. 110-118.
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title = "Copper transfer from Cu-Aβ to human serum albumin inhibits aggregation, radical production and reduces Aβ toxicity",
abstract = "Amyloid-β peptides (Aβ) and the protein human serum albumin (HSA) interact in vivo. They are both localised in the blood plasma and in the cerebrospinal fluid. Among other functions, HSA is involved in the transport of the essential metal copper. Complexes between Aβ and copper ions have been proposed to be an aberrant interaction implicated in the development of Alzheimer's disease, where Cu is involved in Aβ aggregation and production of reactive oxygen species (ROS). In the present work, we studied copper-exchange reaction between Aβ and HSA or the tetrapeptide DAHK (N-terminal Cu-binding domain of HSA) and the consequence of this exchange on Aβ-induced ROS production and cell toxicity. The following results were obtained: 1) HSA and DAHK removed CuII from Ab rapidly and stoichiometrically, 2) HSA and DAHK were able to decrease Cu-induced aggregation of Aβ, 3) HSA and DAHK suppressed the catalytic HO· production in vitro and ROS production in neuroblastoma cells generated by Cu-Aβ and ascorbate, 4) HSA and DAHK were able to rescue these cells from the toxicity of Cu-Aβ with ascorbate, 5) DAHK was more potent in ROS suppression and restoration of neuroblastoma cell viability than HSA, in correlation with an easier reduction of CuII-HSA than Cu-DAHK by ascorbate, in vitro. Our data suggest that HSA is able to decrease aberrant CuII-Aβ interaction. The repercussion of the competition between HSA and Ab to bind Cu in the blood and brain and its relation to Alzheimer's disease are discussed.",
keywords = "Amyloid beta-peptides, Bioinorganic chemistry, Copper, Human serum albumin, Reactive oxygen species",
author = "Lorena Perrone and Emmanuelle Mothes and Maeva Vignes and Ana{\"i}s Mockel and Cindy Figueroa and Miquel, {Marie Christine} and Maddelein, {Marie Lise} and Peter Faller",
year = "2010",
month = "1",
day = "4",
doi = "10.1002/cbic.200900474",
language = "English",
volume = "11",
pages = "110--118",
journal = "ChemBioChem",
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Perrone, L, Mothes, E, Vignes, M, Mockel, A, Figueroa, C, Miquel, MC, Maddelein, ML & Faller, P 2010, 'Copper transfer from Cu-Aβ to human serum albumin inhibits aggregation, radical production and reduces Aβ toxicity', ChemBioChem, bind 11, nr. 1, s. 110-118. https://doi.org/10.1002/cbic.200900474

Copper transfer from Cu-Aβ to human serum albumin inhibits aggregation, radical production and reduces Aβ toxicity. / Perrone, Lorena; Mothes, Emmanuelle; Vignes, Maeva; Mockel, Anaïs; Figueroa, Cindy; Miquel, Marie Christine; Maddelein, Marie Lise; Faller, Peter.

I: ChemBioChem, Bind 11, Nr. 1, 04.01.2010, s. 110-118.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Copper transfer from Cu-Aβ to human serum albumin inhibits aggregation, radical production and reduces Aβ toxicity

AU - Perrone, Lorena

AU - Mothes, Emmanuelle

AU - Vignes, Maeva

AU - Mockel, Anaïs

AU - Figueroa, Cindy

AU - Miquel, Marie Christine

AU - Maddelein, Marie Lise

AU - Faller, Peter

PY - 2010/1/4

Y1 - 2010/1/4

N2 - Amyloid-β peptides (Aβ) and the protein human serum albumin (HSA) interact in vivo. They are both localised in the blood plasma and in the cerebrospinal fluid. Among other functions, HSA is involved in the transport of the essential metal copper. Complexes between Aβ and copper ions have been proposed to be an aberrant interaction implicated in the development of Alzheimer's disease, where Cu is involved in Aβ aggregation and production of reactive oxygen species (ROS). In the present work, we studied copper-exchange reaction between Aβ and HSA or the tetrapeptide DAHK (N-terminal Cu-binding domain of HSA) and the consequence of this exchange on Aβ-induced ROS production and cell toxicity. The following results were obtained: 1) HSA and DAHK removed CuII from Ab rapidly and stoichiometrically, 2) HSA and DAHK were able to decrease Cu-induced aggregation of Aβ, 3) HSA and DAHK suppressed the catalytic HO· production in vitro and ROS production in neuroblastoma cells generated by Cu-Aβ and ascorbate, 4) HSA and DAHK were able to rescue these cells from the toxicity of Cu-Aβ with ascorbate, 5) DAHK was more potent in ROS suppression and restoration of neuroblastoma cell viability than HSA, in correlation with an easier reduction of CuII-HSA than Cu-DAHK by ascorbate, in vitro. Our data suggest that HSA is able to decrease aberrant CuII-Aβ interaction. The repercussion of the competition between HSA and Ab to bind Cu in the blood and brain and its relation to Alzheimer's disease are discussed.

AB - Amyloid-β peptides (Aβ) and the protein human serum albumin (HSA) interact in vivo. They are both localised in the blood plasma and in the cerebrospinal fluid. Among other functions, HSA is involved in the transport of the essential metal copper. Complexes between Aβ and copper ions have been proposed to be an aberrant interaction implicated in the development of Alzheimer's disease, where Cu is involved in Aβ aggregation and production of reactive oxygen species (ROS). In the present work, we studied copper-exchange reaction between Aβ and HSA or the tetrapeptide DAHK (N-terminal Cu-binding domain of HSA) and the consequence of this exchange on Aβ-induced ROS production and cell toxicity. The following results were obtained: 1) HSA and DAHK removed CuII from Ab rapidly and stoichiometrically, 2) HSA and DAHK were able to decrease Cu-induced aggregation of Aβ, 3) HSA and DAHK suppressed the catalytic HO· production in vitro and ROS production in neuroblastoma cells generated by Cu-Aβ and ascorbate, 4) HSA and DAHK were able to rescue these cells from the toxicity of Cu-Aβ with ascorbate, 5) DAHK was more potent in ROS suppression and restoration of neuroblastoma cell viability than HSA, in correlation with an easier reduction of CuII-HSA than Cu-DAHK by ascorbate, in vitro. Our data suggest that HSA is able to decrease aberrant CuII-Aβ interaction. The repercussion of the competition between HSA and Ab to bind Cu in the blood and brain and its relation to Alzheimer's disease are discussed.

KW - Amyloid beta-peptides

KW - Bioinorganic chemistry

KW - Copper

KW - Human serum albumin

KW - Reactive oxygen species

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U2 - 10.1002/cbic.200900474

DO - 10.1002/cbic.200900474

M3 - Journal article

VL - 11

SP - 110

EP - 118

JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

IS - 1

ER -