Construction and screening of marine metagenomic libraries

Nancy Weiland, Carolin Löscher, Rebekka Metzger, Ruth Schmitz

Publikation: Bidrag til bog/antologi/rapport/konference-proceedingBidrag til bog/antologiForskningpeer review

Resumé

Marine microbial communities are highly diverse and have evolved during extended evolutionary processes of physiological adaptations under the influence of a variety of ecological conditions and selection pressures. They harbor an enormous diversity of microbes with still unknown and probably new physiological characteristics. Besides, the surfaces of marine multicellular organisms are typically covered by a consortium of epibiotic bacteria and act as barriers, where diverse interactions between microorganisms and hosts take place. Thus, microbial diversity in the water column of the oceans and the microbial consortia on marine tissues of multicellular organisms are rich sources for isolating novel bioactive compounds and genes. Here we describe the sampling, construction of large-insert metagenomic libraries from marine habitats and exemplarily one function based screen of metagenomic clones.

OriginalsprogEngelsk
TitelMetagenomics : Methods and Protocols
RedaktørerWolfgang R. Streit, Rolf Daniel
Vol/bind668
ForlagSpringer
Publikationsdato2010
Sider51-65
ISBN (Trykt)978-1-60761-822-5
ISBN (Elektronisk)978-1-60761-823-2
DOI
StatusUdgivet - 2010
Udgivet eksterntJa
NavnMethods in Molecular Biology
Vol/bind668
ISSN1064-3745

Fingeraftryk

Metagenomics
Ecosystem
Clone Cells

Citer dette

Weiland, N., Löscher, C., Metzger, R., & Schmitz, R. (2010). Construction and screening of marine metagenomic libraries. I W. R. Streit, & R. Daniel (red.), Metagenomics: Methods and Protocols (Bind 668, s. 51-65). Springer. Methods in Molecular Biology, Bind. 668 https://doi.org/10.1007/978-1-60761-823-2_3
Weiland, Nancy ; Löscher, Carolin ; Metzger, Rebekka ; Schmitz, Ruth. / Construction and screening of marine metagenomic libraries. Metagenomics: Methods and Protocols. red. / Wolfgang R. Streit ; Rolf Daniel. Bind 668 Springer, 2010. s. 51-65 (Methods in Molecular Biology, Bind 668).
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Weiland, N, Löscher, C, Metzger, R & Schmitz, R 2010, Construction and screening of marine metagenomic libraries. i WR Streit & R Daniel (red), Metagenomics: Methods and Protocols. bind 668, Springer, Methods in Molecular Biology, bind 668, s. 51-65. https://doi.org/10.1007/978-1-60761-823-2_3

Construction and screening of marine metagenomic libraries. / Weiland, Nancy; Löscher, Carolin; Metzger, Rebekka; Schmitz, Ruth.

Metagenomics: Methods and Protocols. red. / Wolfgang R. Streit; Rolf Daniel. Bind 668 Springer, 2010. s. 51-65 (Methods in Molecular Biology, Bind 668).

Publikation: Bidrag til bog/antologi/rapport/konference-proceedingBidrag til bog/antologiForskningpeer review

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AB - Marine microbial communities are highly diverse and have evolved during extended evolutionary processes of physiological adaptations under the influence of a variety of ecological conditions and selection pressures. They harbor an enormous diversity of microbes with still unknown and probably new physiological characteristics. Besides, the surfaces of marine multicellular organisms are typically covered by a consortium of epibiotic bacteria and act as barriers, where diverse interactions between microorganisms and hosts take place. Thus, microbial diversity in the water column of the oceans and the microbial consortia on marine tissues of multicellular organisms are rich sources for isolating novel bioactive compounds and genes. Here we describe the sampling, construction of large-insert metagenomic libraries from marine habitats and exemplarily one function based screen of metagenomic clones.

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Weiland N, Löscher C, Metzger R, Schmitz R. Construction and screening of marine metagenomic libraries. I Streit WR, Daniel R, red., Metagenomics: Methods and Protocols. Bind 668. Springer. 2010. s. 51-65. (Methods in Molecular Biology, Bind 668). https://doi.org/10.1007/978-1-60761-823-2_3