Comprehensive transcriptional profiling and mouse phenotyping reveals dispensable role for adipose tissue selective long noncoding RNA Gm15551

Christoph Andreas Engelhard, Chien Huang, Sajjad Khani, Petr Kasparek, Jan Prochazka, Jan Rozman, David Pajuelo Reguera, Radislav Sedlacek, Jan Wilhelm Kornfeld*

*Kontaktforfatter

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Abstract

Cold and nutrient‐activated brown adipose tissue (BAT) is capable of increasing systemic energy expenditure via the uncoupled respiration and secretion of endocrine factors, thereby protecting mice against diet‐induced obesity and improving insulin response and glucose tolerance in men. Long non‐coding RNAs (lncRNAs) have recently been identified as fine‐tuning regulators of cellular function. While certain lncRNAs have been functionally characterised in adipose tissue, their overall contribution in the activation of BAT remains elusive. We identified lncRNAs correlating to interscapular brown adipose tissue (iBAT) function in a high fat diet (HFD) and cold stressed mice. We focused on Gm15551, which has an adipose tissue specific expression profile, is highly upregulated during adipogenesis, and downregulated by β‐adrenergic activation in mature adipocytes. Although we performed comprehensive transcriptional and adipocyte physiology profiling in vitro and in vivo, we could not detect an effect of gain or loss of function of Gm15551.

OriginalsprogEngelsk
Artikelnummer32
TidsskriftNon-coding RNA
Vol/bind8
Udgave nummer3
ISSN2311-553X
DOI
StatusUdgivet - jun. 2022

Bibliografisk note

Funding Information:
Funding: C.H. was funded by the National Taiwan University and the grant 109‐2917‐I‐002‐029 from the Ministry of Science and Technology, Taiwan. The authors used services of the Czech Centre for Phenogenomics supported by the Czech Academy of Sciences RVO 68378050 and by the project LM2018126 Czech Centre for Phenogenomics provided by Ministry of Education, Youth and Sports of the Czech Republic. J.‐W.K. and C.A.E. were supported by the University of Southern Denmark and the Danish Diabetes Academy, which is in turn funded by the Novo Nordisk Foundation.

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

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