Comprehensive small animal imaging strategies on a clinical 3 T dedicated head MR-scanner; adapted methods and sequence protocols in CNS pathologies

Deepu R Pillai, Robin M Heidemann, Praveen Kumar, Nagesh Shanbhag, Titus Lanz, Michael S Dittmar, Beatrice Sandner, Christoph P Beier, Norbert Weidner, Mark W Greenlee, Gerhard Schuierer, Ulrich Bogdahn, Felix Schlachetzki

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

BACKGROUND: Small animal models of human diseases are an indispensable aspect of pre-clinical research. Being dynamic, most pathologies demand extensive longitudinal monitoring to understand disease mechanisms, drug efficacy and side effects. These considerations often demand the concomitant development of monitoring systems with sufficient temporal and spatial resolution.

METHODOLOGY AND RESULTS: This study attempts to configure and optimize a clinical 3 Tesla magnetic resonance scanner to facilitate imaging of small animal central nervous system pathologies. The hardware of the scanner was complemented by a custom-built, 4-channel phased array coil system. Extensive modification of standard sequence protocols was carried out based on tissue relaxometric calculations. Proton density differences between the gray and white matter of the rodent spinal cord along with transverse relaxation due to magnetic susceptibility differences at the cortex and striatum of both rats and mice demonstrated statistically significant differences. The employed parallel imaging reconstruction algorithms had distinct properties dependent on the sequence type and in the presence of the contrast agent. The attempt to morphologically phenotype a normal healthy rat brain in multiple planes delineated a number of anatomical regions, and all the clinically relevant sequels following acute cerebral ischemia could be adequately characterized. Changes in blood-brain-barrier permeability following ischemia-reperfusion were also apparent at a later time. Typical characteristics of intra-cerebral haemorrhage at acute and chronic stages were also visualized up to one month. Two models of rodent spinal cord injury were adequately characterized and closely mimicked the results of histological studies. In the employed rodent animal handling system a mouse model of glioblastoma was also studied with unequivocal results.

CONCLUSIONS: The implemented customizations including extensive sequence protocol modifications resulted in images of high diagnostic quality. These results prove that lack of dedicated animal scanners shouldn't discourage conventional small animal imaging studies.

OriginalsprogEngelsk
TidsskriftPLOS ONE
Vol/bind6
Udgave nummer2
Sider (fra-til)e16091
ISSN1932-6203
DOI
StatusUdgivet - 7. feb. 2011
Udgivet eksterntJa

Fingeraftryk

scanners
Pathology
Animals
rodents
image analysis
ischemia
Imaging techniques
spinal cord
Rodentia
animal models
animal handling
animals
blood-brain barrier
monitoring
rats
Rats
human diseases
protons
central nervous system
hemorrhage

Citer dette

Pillai, Deepu R ; Heidemann, Robin M ; Kumar, Praveen ; Shanbhag, Nagesh ; Lanz, Titus ; Dittmar, Michael S ; Sandner, Beatrice ; Beier, Christoph P ; Weidner, Norbert ; Greenlee, Mark W ; Schuierer, Gerhard ; Bogdahn, Ulrich ; Schlachetzki, Felix. / Comprehensive small animal imaging strategies on a clinical 3 T dedicated head MR-scanner; adapted methods and sequence protocols in CNS pathologies. I: PLOS ONE. 2011 ; Bind 6, Nr. 2. s. e16091.
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Pillai, DR, Heidemann, RM, Kumar, P, Shanbhag, N, Lanz, T, Dittmar, MS, Sandner, B, Beier, CP, Weidner, N, Greenlee, MW, Schuierer, G, Bogdahn, U & Schlachetzki, F 2011, 'Comprehensive small animal imaging strategies on a clinical 3 T dedicated head MR-scanner; adapted methods and sequence protocols in CNS pathologies', PLOS ONE, bind 6, nr. 2, s. e16091. https://doi.org/10.1371/journal.pone.0016091

Comprehensive small animal imaging strategies on a clinical 3 T dedicated head MR-scanner; adapted methods and sequence protocols in CNS pathologies. / Pillai, Deepu R; Heidemann, Robin M; Kumar, Praveen; Shanbhag, Nagesh; Lanz, Titus; Dittmar, Michael S; Sandner, Beatrice; Beier, Christoph P; Weidner, Norbert; Greenlee, Mark W; Schuierer, Gerhard; Bogdahn, Ulrich; Schlachetzki, Felix.

I: PLOS ONE, Bind 6, Nr. 2, 07.02.2011, s. e16091.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Comprehensive small animal imaging strategies on a clinical 3 T dedicated head MR-scanner; adapted methods and sequence protocols in CNS pathologies

AU - Pillai, Deepu R

AU - Heidemann, Robin M

AU - Kumar, Praveen

AU - Shanbhag, Nagesh

AU - Lanz, Titus

AU - Dittmar, Michael S

AU - Sandner, Beatrice

AU - Beier, Christoph P

AU - Weidner, Norbert

AU - Greenlee, Mark W

AU - Schuierer, Gerhard

AU - Bogdahn, Ulrich

AU - Schlachetzki, Felix

PY - 2011/2/7

Y1 - 2011/2/7

N2 - BACKGROUND: Small animal models of human diseases are an indispensable aspect of pre-clinical research. Being dynamic, most pathologies demand extensive longitudinal monitoring to understand disease mechanisms, drug efficacy and side effects. These considerations often demand the concomitant development of monitoring systems with sufficient temporal and spatial resolution.METHODOLOGY AND RESULTS: This study attempts to configure and optimize a clinical 3 Tesla magnetic resonance scanner to facilitate imaging of small animal central nervous system pathologies. The hardware of the scanner was complemented by a custom-built, 4-channel phased array coil system. Extensive modification of standard sequence protocols was carried out based on tissue relaxometric calculations. Proton density differences between the gray and white matter of the rodent spinal cord along with transverse relaxation due to magnetic susceptibility differences at the cortex and striatum of both rats and mice demonstrated statistically significant differences. The employed parallel imaging reconstruction algorithms had distinct properties dependent on the sequence type and in the presence of the contrast agent. The attempt to morphologically phenotype a normal healthy rat brain in multiple planes delineated a number of anatomical regions, and all the clinically relevant sequels following acute cerebral ischemia could be adequately characterized. Changes in blood-brain-barrier permeability following ischemia-reperfusion were also apparent at a later time. Typical characteristics of intra-cerebral haemorrhage at acute and chronic stages were also visualized up to one month. Two models of rodent spinal cord injury were adequately characterized and closely mimicked the results of histological studies. In the employed rodent animal handling system a mouse model of glioblastoma was also studied with unequivocal results.CONCLUSIONS: The implemented customizations including extensive sequence protocol modifications resulted in images of high diagnostic quality. These results prove that lack of dedicated animal scanners shouldn't discourage conventional small animal imaging studies.

AB - BACKGROUND: Small animal models of human diseases are an indispensable aspect of pre-clinical research. Being dynamic, most pathologies demand extensive longitudinal monitoring to understand disease mechanisms, drug efficacy and side effects. These considerations often demand the concomitant development of monitoring systems with sufficient temporal and spatial resolution.METHODOLOGY AND RESULTS: This study attempts to configure and optimize a clinical 3 Tesla magnetic resonance scanner to facilitate imaging of small animal central nervous system pathologies. The hardware of the scanner was complemented by a custom-built, 4-channel phased array coil system. Extensive modification of standard sequence protocols was carried out based on tissue relaxometric calculations. Proton density differences between the gray and white matter of the rodent spinal cord along with transverse relaxation due to magnetic susceptibility differences at the cortex and striatum of both rats and mice demonstrated statistically significant differences. The employed parallel imaging reconstruction algorithms had distinct properties dependent on the sequence type and in the presence of the contrast agent. The attempt to morphologically phenotype a normal healthy rat brain in multiple planes delineated a number of anatomical regions, and all the clinically relevant sequels following acute cerebral ischemia could be adequately characterized. Changes in blood-brain-barrier permeability following ischemia-reperfusion were also apparent at a later time. Typical characteristics of intra-cerebral haemorrhage at acute and chronic stages were also visualized up to one month. Two models of rodent spinal cord injury were adequately characterized and closely mimicked the results of histological studies. In the employed rodent animal handling system a mouse model of glioblastoma was also studied with unequivocal results.CONCLUSIONS: The implemented customizations including extensive sequence protocol modifications resulted in images of high diagnostic quality. These results prove that lack of dedicated animal scanners shouldn't discourage conventional small animal imaging studies.

KW - Animals

KW - Body Size/physiology

KW - Calibration

KW - Central Nervous System Diseases/diagnostic imaging

KW - Disease Models, Animal

KW - Head/diagnostic imaging

KW - Humans

KW - Magnetic Resonance Imaging/instrumentation

KW - Male

KW - Mice

KW - Mice, Nude

KW - Neoplasm Transplantation

KW - Radiography

KW - Rats

KW - Rats, Wistar

KW - Rodentia

KW - Transplantation, Heterologous

KW - Tumor Cells, Cultured

U2 - 10.1371/journal.pone.0016091

DO - 10.1371/journal.pone.0016091

M3 - Journal article

C2 - 21326876

VL - 6

SP - e16091

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 2

ER -