Composition and structure of photosystem I in the moss Physcomitrella patens

Andreas Busch, Jørgen Petersen, Mariam T Webber-Birungi, Marta Powikrowska, Lærke Marie Münter Lassen, Bianca Naumann-Busch, Agnieszka Zygadlo Nielsen, Juanying Ye, Egbert J Boekema, Ole Nørregaard Jensen, Christina Lunde, Poul Erik Jensen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review


Recently, bryophytes, which diverged from the ancestor of seed plants more than 400 million years ago, came into focus in photosynthesis research as they can provide valuable insights into the evolution of photosynthetic complexes during the adaptation to terrestrial life. This study isolated intact photosystem I (PSI) with its associated light-harvesting complex (LHCI) from the moss Physcomitrella patens and characterized its structure, polypeptide composition, and light-harvesting function using electron microscopy, mass spectrometry, biochemical, and physiological methods. It became evident that Physcomitrella possesses a strikingly high number of isoforms for the different PSI core subunits as well as LHCI proteins. It was demonstrated that all these different subunit isoforms are expressed at the protein level and are incorporated into functional PSI-LHCI complexes. Furthermore, in contrast to previous reports, it was demonstrated that Physcomitrella assembles a light-harvesting complex consisting of four light-harvesting proteins forming a higher-plant-like PSI superstructure.
TidsskriftJournal of Experimental Botany. Flowering Newsletter
Udgave nummer10
Sider (fra-til)2689-2699
StatusUdgivet - 2013

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