Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies

Ivana Domljanovic, Annika Carstens, Anders Hauge Okholm, Jørgen Kjems, Christoffer Tandrup Nielsen, Niels H H Heegaard, Kira Astakhova

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Resumé

To date, there are multiple assays developed that detect and quantify antibodies in biofluids. Nevertheless, there is still a lack of simple approaches that specifically detect autoimmune antibodies to double-stranded DNA. Herein we investigate the potential of novel nucleic acid complexes as targets for these antibodies. This is done in a simple, rapid and specific immunofluorescence assay. Specifically, employing 3D nanostructures (DNA origami), we present a new approach in the detection and study of human antibodies to DNA. We demonstrate the detection of anti-DNA antibodies that are characteristic of systemic lupus erythematosus, a chronic autoimmune disease with multiple manifestations. We tested the most potent non-covalent pairs of DNA and fluorescent dyes. Several complexes showed specific recognition of autoimmune antibodies in human samples of lupus patients using a simple one-step immunofluorescence method. This makes the novel assay developed herein a promising tool for research and point-of-care monitoring of anti-DNA antibodies. Using this method, we for the first time experimentally confirm that the disease-specific autoimmune antibodies are sensitive to the 3D structure of nucleic acids and not only to the nucleotide sequence, as was previously thought.

OriginalsprogEngelsk
Artikelnummer1925
TidsskriftScientific Reports
Vol/bind7
Antal sider9
ISSN2045-2322
DOI
StatusUdgivet - 1. dec. 2017

Fingeraftryk

Fluorescent Dyes
DNA
Nucleic Acids
Point-of-Care Systems
Research

Citer dette

Domljanovic, I., Carstens, A., Okholm, A. H., Kjems, J., Nielsen, C. T., Heegaard, N. H. H., & Astakhova, K. (2017). Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies. Scientific Reports, 7, [1925]. https://doi.org/10.1038/s41598-017-02214-0
Domljanovic, Ivana ; Carstens, Annika ; Okholm, Anders Hauge ; Kjems, Jørgen ; Nielsen, Christoffer Tandrup ; Heegaard, Niels H H ; Astakhova, Kira. / Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies. I: Scientific Reports. 2017 ; Bind 7.
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Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies. / Domljanovic, Ivana; Carstens, Annika; Okholm, Anders Hauge; Kjems, Jørgen; Nielsen, Christoffer Tandrup; Heegaard, Niels H H; Astakhova, Kira.

I: Scientific Reports, Bind 7, 1925, 01.12.2017.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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AU - Okholm, Anders Hauge

AU - Kjems, Jørgen

AU - Nielsen, Christoffer Tandrup

AU - Heegaard, Niels H H

AU - Astakhova, Kira

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