Complement-mediated solubilization of immune complexes. Solubilization inhibition and complement factor levels in SLE patients

Gunnar Baatrup, Ivan Petersen, E Kappelgaard, H H Jepsen, Svend-Erik Svehag

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Thirty-two of 36 serum samples from 19 SLE patients showed reduced capacity to mediate complement-dependent solubilization of immune complexes (IC). SLE patients with nephritis exerted the lowest complement-mediated solubilization capacity (CMSC) whereas sera from patients with inactive disease gave the highest CMSC values, with three out of four samples within the normal reference range. Thirty-five of the 36 serum samples showed inhibition of CMSC in a newly developed CMSC inhibition assay. The strongest CMSC inhibition was exerted by sera from newly discovered cases of SLE who received no medical treatment and the lowest inhibition by sera from patients with inactive disease. There was a significant negative correlation between CMSC and CMSC inhibition (r = -0.67, P less than 0.001). Sera with low concentrations of C1q, C3, factor B or high C3d levels showed markedly reduced CMSC values. Pronounced CMSC inhibition was observed only in samples with normal or high factor H values. No significant correlation was found between CMSC or CMSC inhibition and circulating IC levels, but pronounced CMSC inhibition was registered only in strongly IC positive sera.

    OriginalsprogEngelsk
    TidsskriftClinical and Experimental Immunology
    Vol/bind55
    Udgave nummer2
    Sider (fra-til)313-8
    Antal sider6
    ISSN0009-9104
    StatusUdgivet - feb. 1984

    Fingeraftryk

    Serum
    Reference Values
    Complement Factor H
    Nephritis

    Emneord

    • Adolescent
    • Adult
    • Antigen-Antibody Complex
    • Complement Activating Enzymes
    • Complement C1q
    • Complement C3
    • Complement C3b Inactivator Proteins
    • Complement Factor B
    • Complement Factor H
    • Complement System Proteins
    • Female
    • Humans
    • Lupus Erythematosus, Systemic
    • Male
    • Middle Aged
    • Plasmapheresis
    • Solubility

    Citer dette

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    title = "Complement-mediated solubilization of immune complexes. Solubilization inhibition and complement factor levels in SLE patients",
    abstract = "Thirty-two of 36 serum samples from 19 SLE patients showed reduced capacity to mediate complement-dependent solubilization of immune complexes (IC). SLE patients with nephritis exerted the lowest complement-mediated solubilization capacity (CMSC) whereas sera from patients with inactive disease gave the highest CMSC values, with three out of four samples within the normal reference range. Thirty-five of the 36 serum samples showed inhibition of CMSC in a newly developed CMSC inhibition assay. The strongest CMSC inhibition was exerted by sera from newly discovered cases of SLE who received no medical treatment and the lowest inhibition by sera from patients with inactive disease. There was a significant negative correlation between CMSC and CMSC inhibition (r = -0.67, P less than 0.001). Sera with low concentrations of C1q, C3, factor B or high C3d levels showed markedly reduced CMSC values. Pronounced CMSC inhibition was observed only in samples with normal or high factor H values. No significant correlation was found between CMSC or CMSC inhibition and circulating IC levels, but pronounced CMSC inhibition was registered only in strongly IC positive sera.",
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    author = "Gunnar Baatrup and Ivan Petersen and E Kappelgaard and Jepsen, {H H} and Svend-Erik Svehag",
    year = "1984",
    month = "2",
    language = "English",
    volume = "55",
    pages = "313--8",
    journal = "Clinical and Experimental Immunology",
    issn = "0009-9104",
    publisher = "Wiley-Blackwell",
    number = "2",

    }

    Complement-mediated solubilization of immune complexes. Solubilization inhibition and complement factor levels in SLE patients. / Baatrup, Gunnar; Petersen, Ivan; Kappelgaard, E; Jepsen, H H; Svehag, Svend-Erik.

    I: Clinical and Experimental Immunology, Bind 55, Nr. 2, 02.1984, s. 313-8.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Complement-mediated solubilization of immune complexes. Solubilization inhibition and complement factor levels in SLE patients

    AU - Baatrup, Gunnar

    AU - Petersen, Ivan

    AU - Kappelgaard, E

    AU - Jepsen, H H

    AU - Svehag, Svend-Erik

    PY - 1984/2

    Y1 - 1984/2

    N2 - Thirty-two of 36 serum samples from 19 SLE patients showed reduced capacity to mediate complement-dependent solubilization of immune complexes (IC). SLE patients with nephritis exerted the lowest complement-mediated solubilization capacity (CMSC) whereas sera from patients with inactive disease gave the highest CMSC values, with three out of four samples within the normal reference range. Thirty-five of the 36 serum samples showed inhibition of CMSC in a newly developed CMSC inhibition assay. The strongest CMSC inhibition was exerted by sera from newly discovered cases of SLE who received no medical treatment and the lowest inhibition by sera from patients with inactive disease. There was a significant negative correlation between CMSC and CMSC inhibition (r = -0.67, P less than 0.001). Sera with low concentrations of C1q, C3, factor B or high C3d levels showed markedly reduced CMSC values. Pronounced CMSC inhibition was observed only in samples with normal or high factor H values. No significant correlation was found between CMSC or CMSC inhibition and circulating IC levels, but pronounced CMSC inhibition was registered only in strongly IC positive sera.

    AB - Thirty-two of 36 serum samples from 19 SLE patients showed reduced capacity to mediate complement-dependent solubilization of immune complexes (IC). SLE patients with nephritis exerted the lowest complement-mediated solubilization capacity (CMSC) whereas sera from patients with inactive disease gave the highest CMSC values, with three out of four samples within the normal reference range. Thirty-five of the 36 serum samples showed inhibition of CMSC in a newly developed CMSC inhibition assay. The strongest CMSC inhibition was exerted by sera from newly discovered cases of SLE who received no medical treatment and the lowest inhibition by sera from patients with inactive disease. There was a significant negative correlation between CMSC and CMSC inhibition (r = -0.67, P less than 0.001). Sera with low concentrations of C1q, C3, factor B or high C3d levels showed markedly reduced CMSC values. Pronounced CMSC inhibition was observed only in samples with normal or high factor H values. No significant correlation was found between CMSC or CMSC inhibition and circulating IC levels, but pronounced CMSC inhibition was registered only in strongly IC positive sera.

    KW - Adolescent

    KW - Adult

    KW - Antigen-Antibody Complex

    KW - Complement Activating Enzymes

    KW - Complement C1q

    KW - Complement C3

    KW - Complement C3b Inactivator Proteins

    KW - Complement Factor B

    KW - Complement Factor H

    KW - Complement System Proteins

    KW - Female

    KW - Humans

    KW - Lupus Erythematosus, Systemic

    KW - Male

    KW - Middle Aged

    KW - Plasmapheresis

    KW - Solubility

    M3 - Journal article

    C2 - 6230182

    VL - 55

    SP - 313

    EP - 318

    JO - Clinical and Experimental Immunology

    JF - Clinical and Experimental Immunology

    SN - 0009-9104

    IS - 2

    ER -