Comparison of the neutrophil proteome in trauma patients and normal controls

Liz M B Teles, Elaine N Aquino, Anne C D Neves, Carlos H S Garcia, Peter Roepstorff, Belchor Fontes, Mariana S Castro, Wagner Fontes

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Neutrophils have an impressive array of microbicidal weapons, and in the presence of a pathogen, progress from a quiescent state in the bloodstream to a completely activated state. Failure to regulate this activation, for example, when the blood is flooded with cytokines after severe trauma, causes inappropriate neutrophil activation that paradoxically, is associated with tissue and organ damage. Acidic proteomic maps of quiescent human neutrophils were analyzed and compared to those of activated neutrophils from severe trauma patients. The analysis revealed 114 spots whose measured volumes differed between activated and quiescent neutrophils, with 27 upregulated and 87 downregulated in trauma conditions. Among the identified proteins, grancalcin, S100-A9 and CACNB2 reinforce observed correlations between motility and ion flux, ANXA3, SNAP, FGD1 and Zfyve19 are involved in vesicular transport and exocytosis, and GSTP1, HSPA1 HSPA1L, MAOB, UCH-L5, and PPA1 presented evidence that activated neutrophils may have diminished protection against oxidative damage and are prone to apoptosis. These are discussed, along with proteins involved in cytoskeleton reorganization, reactive oxygen species production, and ion flux. Proteins such as Zfyve19, MAOB and albumin- like protein were described for the first time in the neutrophil. In this work we achieved the identification of several proteins potentially involved in inflammatory signaling after trauma, as well as proteins described for the first time in neutrophils.
OriginalsprogEngelsk
TidsskriftProtein and Peptide Letters
Vol/bind19
Udgave nummer6
Sider (fra-til)663-72
Antal sider10
ISSN0929-8665
StatusUdgivet - 2012

Fingeraftryk

Proteome
Neutrophils
Wounds and Injuries
Proteins
Chemical activation
Ions
Fluxes
Pathogens
Neutrophil Activation
Exocytosis
Albumins
Reactive Oxygen Species
Blood
Tissue
Apoptosis
Cytokines
Down-Regulation

Citer dette

Teles, L. M. B., Aquino, E. N., Neves, A. C. D., Garcia, C. H. S., Roepstorff, P., Fontes, B., ... Fontes, W. (2012). Comparison of the neutrophil proteome in trauma patients and normal controls. Protein and Peptide Letters, 19(6), 663-72.
Teles, Liz M B ; Aquino, Elaine N ; Neves, Anne C D ; Garcia, Carlos H S ; Roepstorff, Peter ; Fontes, Belchor ; Castro, Mariana S ; Fontes, Wagner. / Comparison of the neutrophil proteome in trauma patients and normal controls. I: Protein and Peptide Letters. 2012 ; Bind 19, Nr. 6. s. 663-72.
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title = "Comparison of the neutrophil proteome in trauma patients and normal controls",
abstract = "Neutrophils have an impressive array of microbicidal weapons, and in the presence of a pathogen, progress from a quiescent state in the bloodstream to a completely activated state. Failure to regulate this activation, for example, when the blood is flooded with cytokines after severe trauma, causes inappropriate neutrophil activation that paradoxically, is associated with tissue and organ damage. Acidic proteomic maps of quiescent human neutrophils were analyzed and compared to those of activated neutrophils from severe trauma patients. The analysis revealed 114 spots whose measured volumes differed between activated and quiescent neutrophils, with 27 upregulated and 87 downregulated in trauma conditions. Among the identified proteins, grancalcin, S100-A9 and CACNB2 reinforce observed correlations between motility and ion flux, ANXA3, SNAP, FGD1 and Zfyve19 are involved in vesicular transport and exocytosis, and GSTP1, HSPA1 HSPA1L, MAOB, UCH-L5, and PPA1 presented evidence that activated neutrophils may have diminished protection against oxidative damage and are prone to apoptosis. These are discussed, along with proteins involved in cytoskeleton reorganization, reactive oxygen species production, and ion flux. Proteins such as Zfyve19, MAOB and albumin- like protein were described for the first time in the neutrophil. In this work we achieved the identification of several proteins potentially involved in inflammatory signaling after trauma, as well as proteins described for the first time in neutrophils.",
author = "Teles, {Liz M B} and Aquino, {Elaine N} and Neves, {Anne C D} and Garcia, {Carlos H S} and Peter Roepstorff and Belchor Fontes and Castro, {Mariana S} and Wagner Fontes",
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Teles, LMB, Aquino, EN, Neves, ACD, Garcia, CHS, Roepstorff, P, Fontes, B, Castro, MS & Fontes, W 2012, 'Comparison of the neutrophil proteome in trauma patients and normal controls', Protein and Peptide Letters, bind 19, nr. 6, s. 663-72.

Comparison of the neutrophil proteome in trauma patients and normal controls. / Teles, Liz M B; Aquino, Elaine N; Neves, Anne C D; Garcia, Carlos H S; Roepstorff, Peter; Fontes, Belchor; Castro, Mariana S; Fontes, Wagner.

I: Protein and Peptide Letters, Bind 19, Nr. 6, 2012, s. 663-72.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Comparison of the neutrophil proteome in trauma patients and normal controls

AU - Teles, Liz M B

AU - Aquino, Elaine N

AU - Neves, Anne C D

AU - Garcia, Carlos H S

AU - Roepstorff, Peter

AU - Fontes, Belchor

AU - Castro, Mariana S

AU - Fontes, Wagner

PY - 2012

Y1 - 2012

N2 - Neutrophils have an impressive array of microbicidal weapons, and in the presence of a pathogen, progress from a quiescent state in the bloodstream to a completely activated state. Failure to regulate this activation, for example, when the blood is flooded with cytokines after severe trauma, causes inappropriate neutrophil activation that paradoxically, is associated with tissue and organ damage. Acidic proteomic maps of quiescent human neutrophils were analyzed and compared to those of activated neutrophils from severe trauma patients. The analysis revealed 114 spots whose measured volumes differed between activated and quiescent neutrophils, with 27 upregulated and 87 downregulated in trauma conditions. Among the identified proteins, grancalcin, S100-A9 and CACNB2 reinforce observed correlations between motility and ion flux, ANXA3, SNAP, FGD1 and Zfyve19 are involved in vesicular transport and exocytosis, and GSTP1, HSPA1 HSPA1L, MAOB, UCH-L5, and PPA1 presented evidence that activated neutrophils may have diminished protection against oxidative damage and are prone to apoptosis. These are discussed, along with proteins involved in cytoskeleton reorganization, reactive oxygen species production, and ion flux. Proteins such as Zfyve19, MAOB and albumin- like protein were described for the first time in the neutrophil. In this work we achieved the identification of several proteins potentially involved in inflammatory signaling after trauma, as well as proteins described for the first time in neutrophils.

AB - Neutrophils have an impressive array of microbicidal weapons, and in the presence of a pathogen, progress from a quiescent state in the bloodstream to a completely activated state. Failure to regulate this activation, for example, when the blood is flooded with cytokines after severe trauma, causes inappropriate neutrophil activation that paradoxically, is associated with tissue and organ damage. Acidic proteomic maps of quiescent human neutrophils were analyzed and compared to those of activated neutrophils from severe trauma patients. The analysis revealed 114 spots whose measured volumes differed between activated and quiescent neutrophils, with 27 upregulated and 87 downregulated in trauma conditions. Among the identified proteins, grancalcin, S100-A9 and CACNB2 reinforce observed correlations between motility and ion flux, ANXA3, SNAP, FGD1 and Zfyve19 are involved in vesicular transport and exocytosis, and GSTP1, HSPA1 HSPA1L, MAOB, UCH-L5, and PPA1 presented evidence that activated neutrophils may have diminished protection against oxidative damage and are prone to apoptosis. These are discussed, along with proteins involved in cytoskeleton reorganization, reactive oxygen species production, and ion flux. Proteins such as Zfyve19, MAOB and albumin- like protein were described for the first time in the neutrophil. In this work we achieved the identification of several proteins potentially involved in inflammatory signaling after trauma, as well as proteins described for the first time in neutrophils.

M3 - Journal article

VL - 19

SP - 663

EP - 672

JO - Protein and Peptide Letters

JF - Protein and Peptide Letters

SN - 0929-8665

IS - 6

ER -

Teles LMB, Aquino EN, Neves ACD, Garcia CHS, Roepstorff P, Fontes B et al. Comparison of the neutrophil proteome in trauma patients and normal controls. Protein and Peptide Letters. 2012;19(6):663-72.