Comparison of Molecular Recognition of Trimethyllysine and Trimethylthialysine by Epigenetic Reader Proteins

Jordi C J Hintzen, Jordi Poater, Kiran Kumar, Abbas H K Al Temimi, Bas J G E Pieters, Robert S Paton*, F Matthias Bickelhaupt*, Jasmin Mecinović

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Abstrakt

Gaining a fundamental insight into the biomolecular recognition of posttranslationally modified histones by epigenetic reader proteins is of crucial importance to understanding the regulation of the activity of human genes. Here, we seek to establish whether trimethylthialysine, a simple trimethyllysine analogue generated through cysteine alkylation, is a good trimethyllysine mimic for studies on molecular recognition by reader proteins. Histone peptides bearing trimethylthialysine and trimethyllysine were examined for binding with five human reader proteins employing a combination of thermodynamic analyses, molecular dynamics simulations and quantum chemical analyses. Collectively, our experimental and computational findings reveal that trimethylthialysine and trimethyllysine exhibit very similar binding characteristics for the association with human reader proteins, thereby justifying the use of trimethylthialysine for studies aimed at dissecting the origin of biomolecular recognition in epigenetic processes that play important roles in human health and disease.

OriginalsprogEngelsk
Artikelnummer1918
TidsskriftMolecules
Vol/bind25
Udgave nummer8
Antal sider14
ISSN1420-3049
DOI
StatusUdgivet - apr. 2020

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