Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides

E Nordhoff, F Kirpekar, M Karas, R Cramer, S Hahner, F Hillenkamp, K Kristiansen, P Roepstroff, A Lezius

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Abstrakt

UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.

OriginalsprogEngelsk
TidsskriftNucleic acids research
Vol/bind22
Udgave nummer13
Sider (fra-til)2460-5
Antal sider6
ISSN0305-1048
DOI
StatusUdgivet - 11. jul. 1994

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