Comparison of E test and disk diffusion test for antibiotic resistance testing of enterotoxigenic and non-enterotoxigenic Bacteroides fragilis isolated from stools

Mohammad Taghi Akhi, Reza Ghotaslou, Mahnaz Shirinzadeh, Tahereh Pirzadeh, Mohammad Naghavi Behzad

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Background: Enterotoxigenic Bacteroides fragilis (ETBF) are one of the most important anaerobic bacteria which cause diarrhea in human beings. A bft gene is coded for enterotoxin production called fragilysin. Most of them have acquired resistance to antianaerobic bacteria agent like other facultative anaerobic bacteria. E test and different dilution methods are usually performed for antimicrobial susceptibility determination of B. fragilis. Objectives: The aims of this study are to recognize ETBF by PCR method and also to evaluate efficiency the disk diffusion method (DDM) in comparison with the E tests for antimicrobial susceptibility of B. fragilis isolates. Materials and Methods: bft gene was detected among 157 B. fragilis isolated from patients and healthy individuals by PCR. Antimicrobial susceptibility of all isolates was determined by DDM and E tests methods. Results: Nineteen (12.1%) B. fragilis containing bft gene from diarrheic (n = 14) and nondiarrheic (n = 5) feces were detected among 157 B. fragilis isolates. The highest resistance for ciprofoxacin, cefotaxim, cefoxitin with DDM and E test were 100%, 60%, and 65% respectively, for some of antibiotics such as imipenem (P ≤ 0.05), and no correlation for others were observed among the antimicrobial susceptibility while the lowest resistance in two methods was obtained for imipenem, piperacillin/tazobactam, and metronidazol. 100% agreement results obtained by the two methods (P ≥ 0.05). Conclusions: The presence of bft gene in B. fragilis isolates would not certainly result in diarrhea among patients. There is not enough accordance between DDM and Swedish E tests for antimicrobial susceptibility of B. fragilis for some antibiotics, although in other cases a good agreement was observed.

OriginalsprogEngelsk
TidsskriftJundishapur Journal of Microbiology
Vol/bind6
Udgave nummer5
ISSN2008-3645
DOI
StatusUdgivet - 1. jul. 2013

Fingeraftryk

Microbial Drug Resistance
Imipenem
Cefoxitin
Polymerase Chain Reaction
Feces

Citer dette

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title = "Comparison of E test and disk diffusion test for antibiotic resistance testing of enterotoxigenic and non-enterotoxigenic Bacteroides fragilis isolated from stools",
abstract = "Background: Enterotoxigenic Bacteroides fragilis (ETBF) are one of the most important anaerobic bacteria which cause diarrhea in human beings. A bft gene is coded for enterotoxin production called fragilysin. Most of them have acquired resistance to antianaerobic bacteria agent like other facultative anaerobic bacteria. E test and different dilution methods are usually performed for antimicrobial susceptibility determination of B. fragilis. Objectives: The aims of this study are to recognize ETBF by PCR method and also to evaluate efficiency the disk diffusion method (DDM) in comparison with the E tests for antimicrobial susceptibility of B. fragilis isolates. Materials and Methods: bft gene was detected among 157 B. fragilis isolated from patients and healthy individuals by PCR. Antimicrobial susceptibility of all isolates was determined by DDM and E tests methods. Results: Nineteen (12.1{\%}) B. fragilis containing bft gene from diarrheic (n = 14) and nondiarrheic (n = 5) feces were detected among 157 B. fragilis isolates. The highest resistance for ciprofoxacin, cefotaxim, cefoxitin with DDM and E test were 100{\%}, 60{\%}, and 65{\%} respectively, for some of antibiotics such as imipenem (P ≤ 0.05), and no correlation for others were observed among the antimicrobial susceptibility while the lowest resistance in two methods was obtained for imipenem, piperacillin/tazobactam, and metronidazol. 100{\%} agreement results obtained by the two methods (P ≥ 0.05). Conclusions: The presence of bft gene in B. fragilis isolates would not certainly result in diarrhea among patients. There is not enough accordance between DDM and Swedish E tests for antimicrobial susceptibility of B. fragilis for some antibiotics, although in other cases a good agreement was observed.",
keywords = "Antibiotic resistance, Bacteroides fragilis, Disk diffusion antimicrobial tests",
author = "Akhi, {Mohammad Taghi} and Reza Ghotaslou and Mahnaz Shirinzadeh and Tahereh Pirzadeh and {Naghavi Behzad}, Mohammad",
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Comparison of E test and disk diffusion test for antibiotic resistance testing of enterotoxigenic and non-enterotoxigenic Bacteroides fragilis isolated from stools. / Akhi, Mohammad Taghi; Ghotaslou, Reza; Shirinzadeh, Mahnaz; Pirzadeh, Tahereh; Naghavi Behzad, Mohammad.

I: Jundishapur Journal of Microbiology, Bind 6, Nr. 5, 01.07.2013.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Comparison of E test and disk diffusion test for antibiotic resistance testing of enterotoxigenic and non-enterotoxigenic Bacteroides fragilis isolated from stools

AU - Akhi, Mohammad Taghi

AU - Ghotaslou, Reza

AU - Shirinzadeh, Mahnaz

AU - Pirzadeh, Tahereh

AU - Naghavi Behzad, Mohammad

PY - 2013/7/1

Y1 - 2013/7/1

N2 - Background: Enterotoxigenic Bacteroides fragilis (ETBF) are one of the most important anaerobic bacteria which cause diarrhea in human beings. A bft gene is coded for enterotoxin production called fragilysin. Most of them have acquired resistance to antianaerobic bacteria agent like other facultative anaerobic bacteria. E test and different dilution methods are usually performed for antimicrobial susceptibility determination of B. fragilis. Objectives: The aims of this study are to recognize ETBF by PCR method and also to evaluate efficiency the disk diffusion method (DDM) in comparison with the E tests for antimicrobial susceptibility of B. fragilis isolates. Materials and Methods: bft gene was detected among 157 B. fragilis isolated from patients and healthy individuals by PCR. Antimicrobial susceptibility of all isolates was determined by DDM and E tests methods. Results: Nineteen (12.1%) B. fragilis containing bft gene from diarrheic (n = 14) and nondiarrheic (n = 5) feces were detected among 157 B. fragilis isolates. The highest resistance for ciprofoxacin, cefotaxim, cefoxitin with DDM and E test were 100%, 60%, and 65% respectively, for some of antibiotics such as imipenem (P ≤ 0.05), and no correlation for others were observed among the antimicrobial susceptibility while the lowest resistance in two methods was obtained for imipenem, piperacillin/tazobactam, and metronidazol. 100% agreement results obtained by the two methods (P ≥ 0.05). Conclusions: The presence of bft gene in B. fragilis isolates would not certainly result in diarrhea among patients. There is not enough accordance between DDM and Swedish E tests for antimicrobial susceptibility of B. fragilis for some antibiotics, although in other cases a good agreement was observed.

AB - Background: Enterotoxigenic Bacteroides fragilis (ETBF) are one of the most important anaerobic bacteria which cause diarrhea in human beings. A bft gene is coded for enterotoxin production called fragilysin. Most of them have acquired resistance to antianaerobic bacteria agent like other facultative anaerobic bacteria. E test and different dilution methods are usually performed for antimicrobial susceptibility determination of B. fragilis. Objectives: The aims of this study are to recognize ETBF by PCR method and also to evaluate efficiency the disk diffusion method (DDM) in comparison with the E tests for antimicrobial susceptibility of B. fragilis isolates. Materials and Methods: bft gene was detected among 157 B. fragilis isolated from patients and healthy individuals by PCR. Antimicrobial susceptibility of all isolates was determined by DDM and E tests methods. Results: Nineteen (12.1%) B. fragilis containing bft gene from diarrheic (n = 14) and nondiarrheic (n = 5) feces were detected among 157 B. fragilis isolates. The highest resistance for ciprofoxacin, cefotaxim, cefoxitin with DDM and E test were 100%, 60%, and 65% respectively, for some of antibiotics such as imipenem (P ≤ 0.05), and no correlation for others were observed among the antimicrobial susceptibility while the lowest resistance in two methods was obtained for imipenem, piperacillin/tazobactam, and metronidazol. 100% agreement results obtained by the two methods (P ≥ 0.05). Conclusions: The presence of bft gene in B. fragilis isolates would not certainly result in diarrhea among patients. There is not enough accordance between DDM and Swedish E tests for antimicrobial susceptibility of B. fragilis for some antibiotics, although in other cases a good agreement was observed.

KW - Antibiotic resistance

KW - Bacteroides fragilis

KW - Disk diffusion antimicrobial tests

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U2 - 10.5812/jjm.9800

DO - 10.5812/jjm.9800

M3 - Journal article

VL - 6

JO - Jundishapur Journal of Microbiology

JF - Jundishapur Journal of Microbiology

SN - 2008-3645

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