Comparison between various biomarkers of senescence in bone marrow-derived stromal cells in vitro and ex-vivo

Jan Nehlin, Moustapha Kassem, Charles Frary

Publikation: Konferencebidrag uden forlag/tidsskriftKonferenceabstrakt til konferenceForskningpeer review

Abstrakt

Senescent stem cells are classified as non-quiescent, irreversibly growth-arrested, non-terminally differentiated, apoptosis resistant multipotent stem cells that maintain an altered gene expression from their juvenescent precursors. Established markers of senescence such as senescent-associated β-galactosidase, p16, and senescent-associated heterochromatic foci (SAHF) can only be analyzed through the use of cell toxic stains or fixatives while BOCS, biomarker of cellular senescence, along with certain morphological qualities can be visualized and quantified without inflicting any damage to cellular structures.
Bone marrow-derived stromal cells were isolated from young and old healthy subjects and cultured to senescence. The senescent cells were compared to their passage 1 counterparts through fluorescent high-throughput examination of C12FDG, SAHF, p16, BOCS stainings and morphology. This analysis was then repeated on passage 1 alone from both young and old healthy donors to examine the effect of donor age on biomarkers ex-vivo.
Cellular C12FDG staining, morphology, SAHF and nuclear p16 expression were increased similarly to BOCS from early to late passages. When bone marrow-derived stromal cells from young and old healthy subjects were compared ex-vivo, BOCS was the only biomarker found to be significantly up-regulated showing that BOCS correlates with the senescent phenotype at least as well as C12FDG and nuclear p16 stainings but without fixation or permeabilization.

OriginalsprogEngelsk
Publikationsdato6. sep. 2013
StatusUdgivet - 6. sep. 2013
BegivenhedStress and Ageing: from Molecular Biology to Clinical Perspectives - 10 years ageing meeting in Halle - Martin-Luther-University, Halle-Wittenberg, Germany, Danmark
Varighed: 6. sep. 20138. sep. 2013

Konference

KonferenceStress and Ageing: from Molecular Biology to Clinical Perspectives - 10 years ageing meeting in Halle
LokationMartin-Luther-University
LandDanmark
ByHalle-Wittenberg, Germany
Periode06/09/201308/09/2013

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Citationsformater

Nehlin, J., Kassem, M., & Frary, C. (2013). Comparison between various biomarkers of senescence in bone marrow-derived stromal cells in vitro and ex-vivo. Abstract fra Stress and Ageing: from Molecular Biology to Clinical Perspectives - 10 years ageing meeting in Halle, Halle-Wittenberg, Germany, Danmark.