Comparing Peptide Spectra Matches Across Search Engines

Rune Matthiesen, Gorka Prieto, Hans Christian Beck

Publikation: Kapitel i bog/rapport/konference-proceedingKapitel i bogForskningpeer review

Abstrakt

Mass spectrometry is extremely efficient for sequencing small peptides generated by, for example, a trypsin digestion of a complex mixture. Current instruments have the capacity to generate 50-100 K MSMS spectra from a single run. Of these ~30-50% is typically assigned to peptide matches on a 1% FDR threshold. The remaining spectra need more research to explain. We address here whether the 30-50% matched spectra provide consensus matches when using different database-dependent search pipelines. Although the majority of the spectra peptide assignments concur across search engines, our conclusion is that database-dependent search engines still require improvements.

OriginalsprogEngelsk
TitelMass Spectrometry Data Analysis in Proteomics
RedaktørerRune Matthiesen
ForlagHumana Press
Publikationsdato2020
Udgave3.
Sider133-143
ISBN (Trykt)9781493997435
ISBN (Elektronisk)9781493997442
DOI
StatusUdgivet - 2020
NavnMethods in Molecular Biology
Vol/bind2051
ISSN1064-3745

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