Comparing biological and pathological factors affecting osteocalcin concentrations in archaeological skeletal remains

Amy B. Scott*, Alberto Taurozzi, Nicole Hughes, Dorthe Dangvard Pedersen, Ioannis Kontopoulos, Meaghan Mackie, Matthew J. Collins

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

19 Downloads (Pure)

Abstrakt

Paleoproteomics and the study of ancient proteins has become an important consideration in bioarchaeological research as we seek to understand the relationship between the physical skeleton and its underlying biochemistry. Osteocalcin is an abundant, non-collagenous protein that is accessible archaeologically due to its affinity for hydroxyapatite in bone. Manufactured by the osteoblast cells during normal bone metabolism, osteocalcin is inherently linked to the process of bone remodelling. Clinical studies have consistently shown the impact of biological (i.e., sex and age) and pathological (i.e., disease) factors on osteocalcin concentrations within the body; however, this connection has yet to be fully explored bioarchaeologically. For this study, 46 individuals from the Danish Sct. Albert's cemetery (CE 1250–1536) were analyzed with cortical bone samples harvested from the femur. Each sample was demineralized and quantified using established paleoproteomic methods (ELISA) and tested for diagenesis using FTIR-ATR. No significant differences were found in the osteocalcin concentrations between adult males and females. However, males had a wider range of osteocalcin variability perhaps explained in part by their increased prevalence of pathological conditions characterized by bone remodelling. Similarly, non-adults had a wide range of osteocalcin variability but there were no significant differences when compared to the adult mean. No correlations were found between osteocalcin concentrations and diagenesis suggesting that the protein can survive across a range of temporal periods and variable burial environments. While no clear pattern emerged between osteocalcin concentrations and biological and/or pathological factors, this study is significant in that it further explores the use of paleoproteomics in bioarchaeological contexts and how we may unite our macroscopic and biochemical assessments of human skeletal remains.

OriginalsprogEngelsk
Artikelnummer102573
TidsskriftJournal of Archaeological Science: Reports
Vol/bind34
Udgave nummerPart A
Antal sider9
ISSN2352-409X
DOI
StatusUdgivet - dec. 2020

Fingeraftryk

Dyk ned i forskningsemnerne om 'Comparing biological and pathological factors affecting osteocalcin concentrations in archaeological skeletal remains'. Sammen danner de et unikt fingeraftryk.

Citationsformater