Combined subcutaneous fat aspirate and skin tru-cut biopsy for amyloid screening in patients with suspected systemic amyloidosis

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

35 Downloads (Pure)

Abstract

Screening for systemic amyloidosis is typically carried out with abdominal fat aspirates with varying reported sensitivities. Fat aspirates are preferred for use in primary screening instead of organ biopsies as they are less invasive and thereby minimize the potential risk of complications. At Odense Amyloidosis Center, we performed a prospective study on whether the combined use of fat aspirate and tru-cut skin biopsy could increase the diagnostic sensitivity. Both fat aspirates and skin biopsies were screened with Congo Red staining, and positive biopsies were subsequently subtyped using immunoelectron microscopy and mass spectrometry. Seventy-six patients were included. In total, 24 patients had systemic amyloidosis (11 AL, 12 wtATTR, 1 AA), and 6 patients had localized amyloidosis. Combined fat aspirate and skin biopsy were Congo Red-positive in 15 patients (overall sensitivity (OS) 62.5%). Fat aspirates were positive in 14 patients (OS 58.3%), and the skin biopsy was positive in 5 patients (OS 20.8%). In only one patient did the skin biopsy add extra diagnostic information. The sensitivity differed between AL and ATTR amyloidosis—81.8% and 41.7%, respectively. Using skin biopsy as the only screening method is not recommended.

OriginalsprogEngelsk
Artikelnummer3649
TidsskriftMolecules
Vol/bind26
Udgave nummer12
Antal sider7
ISSN1420-3049
DOI
StatusUdgivet - 15. jun. 2021

Bibliografisk note

Funding Information:
This research was partly funded by a ?center of clinical excellence? research grant from the Health Region of Southern Denmark to Odense Amyloidosis Center.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Fingeraftryk

Dyk ned i forskningsemnerne om 'Combined subcutaneous fat aspirate and skin tru-cut biopsy for amyloid screening in patients with suspected systemic amyloidosis'. Sammen danner de et unikt fingeraftryk.

Citationsformater