Colonization and biofilm formation by Staphylococcus aureus on endothelial cell layers under flow

Publikation: Konferencebidrag uden forlag/tidsskriftKonferenceabstrakt til konferenceForskningpeer review

Resumé

Staphylococcus aureus is a major human pathogen and known for causing vascular infections such as sepsis and infective endocarditis. It has previously been proposed that S. aureus succeed in colonization of the endothelial wall by specific surface attachment likely followed by biofilm formation. Furthermore, S. aureus is known to invade human cells, which has been proposed to promote persistence through immune and antibiotic evasion. In the current study, we sought to investigate endothelial colonization, invasion, and biofilm formation by S. aureus using a newly developed in vitro flow chamber model. We show that under physiological shear rates, S. aureus utilizes cellular invasion to enable the following surface colonization and biofilm formation. These observations might help explain the success of S. aureus as a bloodstream pathogen and guide further studies in S. aureus pathogenesis and treatment of S. aureus biofilms.
OriginalsprogEngelsk
Publikationsdato27. maj 2018
Antal sider1
StatusUdgivet - 27. maj 2018
BegivenhedBiofilms8 - Aarhus University, Aarhus, Danmark
Varighed: 27. maj 201829. maj 2018
http://conferences.au.dk/biofilms8/

Konference

KonferenceBiofilms8
LokationAarhus University
LandDanmark
ByAarhus
Periode27/05/201829/05/2018
Internetadresse

Fingeraftryk

Biofilms

Citer dette

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abstract = "Staphylococcus aureus is a major human pathogen and known for causing vascular infections such as sepsis and infective endocarditis. It has previously been proposed that S. aureus succeed in colonization of the endothelial wall by specific surface attachment likely followed by biofilm formation. Furthermore, S. aureus is known to invade human cells, which has been proposed to promote persistence through immune and antibiotic evasion. In the current study, we sought to investigate endothelial colonization, invasion, and biofilm formation by S. aureus using a newly developed in vitro flow chamber model. We show that under physiological shear rates, S. aureus utilizes cellular invasion to enable the following surface colonization and biofilm formation. These observations might help explain the success of S. aureus as a bloodstream pathogen and guide further studies in S. aureus pathogenesis and treatment of S. aureus biofilms.",
keywords = "Biofilms, Pathogens, Staphylococcus aureus",
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Colonization and biofilm formation by Staphylococcus aureus on endothelial cell layers under flow. / Grønnemose, Rasmus Birkholm; Antoinette Asferg, Cecilie; Kolmos, Hans Jørn; Andersen, Thomas Emil.

2018. Abstract fra Biofilms8, Aarhus, Danmark.

Publikation: Konferencebidrag uden forlag/tidsskriftKonferenceabstrakt til konferenceForskningpeer review

TY - ABST

T1 - Colonization and biofilm formation by Staphylococcus aureus on endothelial cell layers under flow

AU - Grønnemose, Rasmus Birkholm

AU - Antoinette Asferg, Cecilie

AU - Kolmos, Hans Jørn

AU - Andersen, Thomas Emil

PY - 2018/5/27

Y1 - 2018/5/27

N2 - Staphylococcus aureus is a major human pathogen and known for causing vascular infections such as sepsis and infective endocarditis. It has previously been proposed that S. aureus succeed in colonization of the endothelial wall by specific surface attachment likely followed by biofilm formation. Furthermore, S. aureus is known to invade human cells, which has been proposed to promote persistence through immune and antibiotic evasion. In the current study, we sought to investigate endothelial colonization, invasion, and biofilm formation by S. aureus using a newly developed in vitro flow chamber model. We show that under physiological shear rates, S. aureus utilizes cellular invasion to enable the following surface colonization and biofilm formation. These observations might help explain the success of S. aureus as a bloodstream pathogen and guide further studies in S. aureus pathogenesis and treatment of S. aureus biofilms.

AB - Staphylococcus aureus is a major human pathogen and known for causing vascular infections such as sepsis and infective endocarditis. It has previously been proposed that S. aureus succeed in colonization of the endothelial wall by specific surface attachment likely followed by biofilm formation. Furthermore, S. aureus is known to invade human cells, which has been proposed to promote persistence through immune and antibiotic evasion. In the current study, we sought to investigate endothelial colonization, invasion, and biofilm formation by S. aureus using a newly developed in vitro flow chamber model. We show that under physiological shear rates, S. aureus utilizes cellular invasion to enable the following surface colonization and biofilm formation. These observations might help explain the success of S. aureus as a bloodstream pathogen and guide further studies in S. aureus pathogenesis and treatment of S. aureus biofilms.

KW - Biofilms

KW - Pathogens

KW - Staphylococcus aureus

UR - http://conferences.au.dk/biofilms8/

M3 - Conference abstract for conference

ER -